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Genetic and Phytochemical Analysis to Evaluate the Diversity and Relationships of Mate (Ilex paraguariensis A.St.-Hil.) Elite Genetic Resources in a Germplasm Collection.
Chem Biodivers. 2017 Mar; 14(3)CB

Abstract

The aim of this study was to evaluate the phytochemical and genetic diversity, relationships and identification of mate (Ilex paraguariensis A.St.-Hil.) elite genetic resources belonging to the Brazilian germplasm collection and mate breeding program. Mate has been studied due to the presence of phytochemical compounds, especially methylxanthines and phenolic compounds. The samples were collected from the leaves of 76 mate elite genetic resources (16 progenies × 5 localities). Total DNA was extracted from mate leaves and 20 random primers were used for DNA amplification. Methylxanthines (caffeine and theobromine) and phenolic compounds (chlorogenic, neochlorogenic, and criptochlorogenic acids) were quantified by HPLC. The genetic divergence estimated was higher within (92%) than among (8%) the different populations. Analysis of genetic distance between origins provided the formation of two groups by UPGMA cluster analysis, with higher polymorphism (94.9%). The average content of caffeine ranged from 0.01 to 1.38% and theobromine of 0.10 - 0.85% (w/w). The caffeoylquinic acids concentrations (1.43 - 5.38%) showed a gradient 3-CQA > 5-CQA > 4-CQA. The coefficient of genetic variation (CVg) was of low magnitude for all mono-caffeoylquinics acids. Significant correlations (positive and negative) were observed between the phytochemical compounds. Genetic diversity analysis performed by RAPD markers showed a greater intra-populational diversity; genetic resources with low caffeine and higher theobromine content were identified and can be used in breeding programs; the correlation between methylxanthines and phenolic compounds can be used as a good predictor in future studies.

Authors+Show Affiliations

Chemistry and Pharmacology of Natural Products Laboratory, Institute of Biological, Medical and Health Sciences, Universidade Paranaense (UNIPAR), Av. Parigot de Souza 3636, Toledo, PR, 85.903-170, Brazil.Agronomy Department, Universidade Estadual de Maringá (UEM), Av. Colombo 5790 - Vila Esperança, Maringá, PR, 87.020-900, Brazil.Agronomy Department, Universidade Estadual de Maringá (UEM), Av. Colombo 5790 - Vila Esperança, Maringá, PR, 87.020-900, Brazil.Agronomy Department, Universidade Estadual de Maringá (UEM), Av. Colombo 5790 - Vila Esperança, Maringá, PR, 87.020-900, Brazil.Embrapa Florestas - Brazilian Agricultural Research Corporation (EMBRAPA), Km 111, Estrada da Ribeira, Colombo, PR, 83.411-000, Brazil.Chemistry and Pharmacology of Natural Products Laboratory, Institute of Biological, Medical and Health Sciences, Universidade Paranaense (UNIPAR), Av. Parigot de Souza 3636, Toledo, PR, 85.903-170, Brazil.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27701846

Citation

Friedrich, Juliana Cristhina, et al. "Genetic and Phytochemical Analysis to Evaluate the Diversity and Relationships of Mate (Ilex Paraguariensis A.St.-Hil.) Elite Genetic Resources in a Germplasm Collection." Chemistry & Biodiversity, vol. 14, no. 3, 2017.
Friedrich JC, Gonela A, Gonçalves Vidigal MC, et al. Genetic and Phytochemical Analysis to Evaluate the Diversity and Relationships of Mate (Ilex paraguariensis A.St.-Hil.) Elite Genetic Resources in a Germplasm Collection. Chem Biodivers. 2017;14(3).
Friedrich, J. C., Gonela, A., Gonçalves Vidigal, M. C., Vidigal Filho, P. S., Sturion, J. A., & Cardozo Junior, E. L. (2017). Genetic and Phytochemical Analysis to Evaluate the Diversity and Relationships of Mate (Ilex paraguariensis A.St.-Hil.) Elite Genetic Resources in a Germplasm Collection. Chemistry & Biodiversity, 14(3). https://doi.org/10.1002/cbdv.201600177
Friedrich JC, et al. Genetic and Phytochemical Analysis to Evaluate the Diversity and Relationships of Mate (Ilex Paraguariensis A.St.-Hil.) Elite Genetic Resources in a Germplasm Collection. Chem Biodivers. 2017;14(3) PubMed PMID: 27701846.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genetic and Phytochemical Analysis to Evaluate the Diversity and Relationships of Mate (Ilex paraguariensis A.St.-Hil.) Elite Genetic Resources in a Germplasm Collection. AU - Friedrich,Juliana Cristhina, AU - Gonela,Adriana, AU - Gonçalves Vidigal,Maria Celeste, AU - Vidigal Filho,Pedro Soares, AU - Sturion,José Alfredo, AU - Cardozo Junior,Euclides Lara, Y1 - 2017/02/16/ PY - 2016/06/03/received PY - 2016/09/30/accepted PY - 2016/10/5/pubmed PY - 2017/4/11/medline PY - 2016/10/5/entrez KW - Ilex paraguariensis KW - Genetic diversity KW - Mate KW - Methylxanthines KW - Phenolic compounds KW - Phytochemical composition JF - Chemistry & biodiversity JO - Chem Biodivers VL - 14 IS - 3 N2 - The aim of this study was to evaluate the phytochemical and genetic diversity, relationships and identification of mate (Ilex paraguariensis A.St.-Hil.) elite genetic resources belonging to the Brazilian germplasm collection and mate breeding program. Mate has been studied due to the presence of phytochemical compounds, especially methylxanthines and phenolic compounds. The samples were collected from the leaves of 76 mate elite genetic resources (16 progenies × 5 localities). Total DNA was extracted from mate leaves and 20 random primers were used for DNA amplification. Methylxanthines (caffeine and theobromine) and phenolic compounds (chlorogenic, neochlorogenic, and criptochlorogenic acids) were quantified by HPLC. The genetic divergence estimated was higher within (92%) than among (8%) the different populations. Analysis of genetic distance between origins provided the formation of two groups by UPGMA cluster analysis, with higher polymorphism (94.9%). The average content of caffeine ranged from 0.01 to 1.38% and theobromine of 0.10 - 0.85% (w/w). The caffeoylquinic acids concentrations (1.43 - 5.38%) showed a gradient 3-CQA > 5-CQA > 4-CQA. The coefficient of genetic variation (CVg) was of low magnitude for all mono-caffeoylquinics acids. Significant correlations (positive and negative) were observed between the phytochemical compounds. Genetic diversity analysis performed by RAPD markers showed a greater intra-populational diversity; genetic resources with low caffeine and higher theobromine content were identified and can be used in breeding programs; the correlation between methylxanthines and phenolic compounds can be used as a good predictor in future studies. SN - 1612-1880 UR - https://www.unboundmedicine.com/medline/citation/27701846/Genetic_and_Phytochemical_Analysis_to_Evaluate_the_Diversity_and_Relationships_of_Mate__Ilex_paraguariensis_A_St__Hil___Elite_Genetic_Resources_in_a_Germplasm_Collection_ DB - PRIME DP - Unbound Medicine ER -