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Rapid isolation with Sep-Pak C18 cartridges and wide-bore capillary gas chromatography of some barbiturates.
Med Sci Law. 1989 Jul; 29(3):242-8.MS

Abstract

A simple and rapid method for isolation of nine barbiturates with Sep-Pak C18 cartridges from human urine, plasma and whole blood, is presented; the detection of drugs was made by wide-bore capillary gas chromatography (GC) with flame ionization. The drug-containing samples, after mixing with dilute acid solution, were directly applied to the cartridges and eluted with either chloroform/methanol (9:1) or acetonitrile. Separation of the nine drugs was satisfactory with use of an intermediately polar HP-17 capillary column. Recoveries of most compounds were excellent for both chloroform/methanol and acetonitrile as elution solvents. However, backgrounds were cleaner and evaporation time was much shorter for the chloroform/methanol system. The present isolation method with use of Sep-Pak C18 cartridges and wide-bore capillary GC seem very useful in the fields of forensic chemistry, clinical toxicology and clinical pharmacology.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

2770475

Citation

Suzuki, O, et al. "Rapid Isolation With Sep-Pak C18 Cartridges and Wide-bore Capillary Gas Chromatography of some Barbiturates." Medicine, Science, and the Law, vol. 29, no. 3, 1989, pp. 242-8.
Suzuki O, Kumazawa T, Seno H, et al. Rapid isolation with Sep-Pak C18 cartridges and wide-bore capillary gas chromatography of some barbiturates. Med Sci Law. 1989;29(3):242-8.
Suzuki, O., Kumazawa, T., Seno, H., & Hattori, H. (1989). Rapid isolation with Sep-Pak C18 cartridges and wide-bore capillary gas chromatography of some barbiturates. Medicine, Science, and the Law, 29(3), 242-8.
Suzuki O, et al. Rapid Isolation With Sep-Pak C18 Cartridges and Wide-bore Capillary Gas Chromatography of some Barbiturates. Med Sci Law. 1989;29(3):242-8. PubMed PMID: 2770475.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rapid isolation with Sep-Pak C18 cartridges and wide-bore capillary gas chromatography of some barbiturates. AU - Suzuki,O, AU - Kumazawa,T, AU - Seno,H, AU - Hattori,H, PY - 1989/7/1/pubmed PY - 1989/7/1/medline PY - 1989/7/1/entrez SP - 242 EP - 8 JF - Medicine, science, and the law JO - Med Sci Law VL - 29 IS - 3 N2 - A simple and rapid method for isolation of nine barbiturates with Sep-Pak C18 cartridges from human urine, plasma and whole blood, is presented; the detection of drugs was made by wide-bore capillary gas chromatography (GC) with flame ionization. The drug-containing samples, after mixing with dilute acid solution, were directly applied to the cartridges and eluted with either chloroform/methanol (9:1) or acetonitrile. Separation of the nine drugs was satisfactory with use of an intermediately polar HP-17 capillary column. Recoveries of most compounds were excellent for both chloroform/methanol and acetonitrile as elution solvents. However, backgrounds were cleaner and evaporation time was much shorter for the chloroform/methanol system. The present isolation method with use of Sep-Pak C18 cartridges and wide-bore capillary GC seem very useful in the fields of forensic chemistry, clinical toxicology and clinical pharmacology. SN - 0025-8024 UR - https://www.unboundmedicine.com/medline/citation/2770475/Rapid_isolation_with_Sep_Pak_C18_cartridges_and_wide_bore_capillary_gas_chromatography_of_some_barbiturates_ L2 - https://journals.sagepub.com/doi/10.1177/002580248902900309?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -
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