Tags

Type your tag names separated by a space and hit enter

Quantitative and Qualitative Analysis of Flavonoids and Phenolic Acids in Snow Chrysanthemum (Coreopsis tinctoria Nutt.) by HPLC-DAD and UPLC-ESI-QTOF-MS.
Molecules. 2016 Sep 30; 21(10)M

Abstract

A simple, accurate and reliable high performance liquid chromatography coupled with photodiode array detection (HPLC-DAD) method was developed and then successfully applied for simultaneous quantitative analysis of eight compounds, including chlorogenic acid (1), (R/S)-flavanomarein (2), butin-7-O-β-d-glucopyranoside (3), isookanin (4), taxifolin (5), 5,7,3',5'-tetrahydroxyflavanone-7-O-β-d-glucopyranoside (6), marein (7) and okanin (8), in 23 batches of snow chrysanthemum of different seed provenance and from various habitats. The results showed total contents of the eight compounds in the samples with seed provenance from Keliyang (Xinjiang, China), are higher than in samples from the other five provenances by 52.47%, 15.53%, 19.78%, 21.17% and 5.06%, respectively, which demonstrated that provenance has a great influence on the constituents in snow chrysanthemum. Meanwhile, an ultra performance liquid chromatography coupled with electrospray ionization and quadrupole time-of-flight-mass spectrometry (UPLC-ESI-QTOF-MS) was also employed to rapidly separate and identify flavonoids and phenolic acids in snow chrysanthemum from Keliyang. As a result, a total of 30 constituents, including 26 flavonoids and four phenolic acids, were identified or tentatively identified based on the exact mass information, the fragmentation characteristics, and retention times of eight reference standards. This work may provide an efficient approach to comprehensively evaluate the quality of snow chrysanthemum.

Authors+Show Affiliations

Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China. iyangyinjun@163.com. Department of Biology, Beijing Institute of Radiation Medicine, Beijing 100850, China. iyangyinjun@163.com.Department of Biology, Beijing Institute of Radiation Medicine, Beijing 100850, China. sxgzhwu07@163.com.Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China. liujinjun08@163.com.Department of Biology, Beijing Institute of Radiation Medicine, Beijing 100850, China. kang_liping21@163.com.Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China. sibao.chen@polyu.edu.hk.Department of Biology, Beijing Institute of Radiation Medicine, Beijing 100850, China. mabaiping@sina.com.Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China. guobaolin010@163.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27706037

Citation

Yang, Yinjun, et al. "Quantitative and Qualitative Analysis of Flavonoids and Phenolic Acids in Snow Chrysanthemum (Coreopsis Tinctoria Nutt.) By HPLC-DAD and UPLC-ESI-QTOF-MS." Molecules (Basel, Switzerland), vol. 21, no. 10, 2016.
Yang Y, Sun X, Liu J, et al. Quantitative and Qualitative Analysis of Flavonoids and Phenolic Acids in Snow Chrysanthemum (Coreopsis tinctoria Nutt.) by HPLC-DAD and UPLC-ESI-QTOF-MS. Molecules. 2016;21(10).
Yang, Y., Sun, X., Liu, J., Kang, L., Chen, S., Ma, B., & Guo, B. (2016). Quantitative and Qualitative Analysis of Flavonoids and Phenolic Acids in Snow Chrysanthemum (Coreopsis tinctoria Nutt.) by HPLC-DAD and UPLC-ESI-QTOF-MS. Molecules (Basel, Switzerland), 21(10).
Yang Y, et al. Quantitative and Qualitative Analysis of Flavonoids and Phenolic Acids in Snow Chrysanthemum (Coreopsis Tinctoria Nutt.) By HPLC-DAD and UPLC-ESI-QTOF-MS. Molecules. 2016 Sep 30;21(10) PubMed PMID: 27706037.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quantitative and Qualitative Analysis of Flavonoids and Phenolic Acids in Snow Chrysanthemum (Coreopsis tinctoria Nutt.) by HPLC-DAD and UPLC-ESI-QTOF-MS. AU - Yang,Yinjun, AU - Sun,Xinguang, AU - Liu,Jinjun, AU - Kang,Liping, AU - Chen,Sibao, AU - Ma,Baiping, AU - Guo,Baolin, Y1 - 2016/09/30/ PY - 2016/08/26/received PY - 2016/09/22/revised PY - 2016/09/25/accepted PY - 2016/10/6/entrez PY - 2016/10/6/pubmed PY - 2017/4/1/medline KW - HPLC-DAD KW - UPLC-ESI-QTOF-MS KW - flavonoids and phenolic acids KW - provenance and habitat KW - quality evaluation KW - snow chrysanthemum JF - Molecules (Basel, Switzerland) JO - Molecules VL - 21 IS - 10 N2 - A simple, accurate and reliable high performance liquid chromatography coupled with photodiode array detection (HPLC-DAD) method was developed and then successfully applied for simultaneous quantitative analysis of eight compounds, including chlorogenic acid (1), (R/S)-flavanomarein (2), butin-7-O-β-d-glucopyranoside (3), isookanin (4), taxifolin (5), 5,7,3',5'-tetrahydroxyflavanone-7-O-β-d-glucopyranoside (6), marein (7) and okanin (8), in 23 batches of snow chrysanthemum of different seed provenance and from various habitats. The results showed total contents of the eight compounds in the samples with seed provenance from Keliyang (Xinjiang, China), are higher than in samples from the other five provenances by 52.47%, 15.53%, 19.78%, 21.17% and 5.06%, respectively, which demonstrated that provenance has a great influence on the constituents in snow chrysanthemum. Meanwhile, an ultra performance liquid chromatography coupled with electrospray ionization and quadrupole time-of-flight-mass spectrometry (UPLC-ESI-QTOF-MS) was also employed to rapidly separate and identify flavonoids and phenolic acids in snow chrysanthemum from Keliyang. As a result, a total of 30 constituents, including 26 flavonoids and four phenolic acids, were identified or tentatively identified based on the exact mass information, the fragmentation characteristics, and retention times of eight reference standards. This work may provide an efficient approach to comprehensively evaluate the quality of snow chrysanthemum. SN - 1420-3049 UR - https://www.unboundmedicine.com/medline/citation/27706037/Quantitative_and_Qualitative_Analysis_of_Flavonoids_and_Phenolic_Acids_in_Snow_Chrysanthemum__Coreopsis_tinctoria_Nutt___by_HPLC_DAD_and_UPLC_ESI_QTOF_MS_ L2 - https://www.mdpi.com/resolver?pii=molecules21101307 DB - PRIME DP - Unbound Medicine ER -