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Detection of 'Candidatus Neoehrlichia mikurensis' and other Anaplasmataceae and Rickettsiaceae in Canidae in Switzerland and Mediterranean countries.
Schweiz Arch Tierheilkd. 2016 Oct; 158(10):691-700.SA

Abstract

INTRODUCTION

'Candidatus Neoehrlichia mikurensis' is an emerging tick-borne zoonotic agent that primarily affects immunocompromised human patients. Dogs and foxes are frequently exposed to ticks, and both species are in close proximity to humans. This is the first study to systematically investigate the occurrence of 'Candidatus Neoehrlichia mikurensis' in Canidae in Europa. We analyzed 1'739 blood samples from dogs in Switzerland, Italy, Spain and Portugal and 162 blood samples from free-ranging red foxes (Vulpes vulpes) in Switzerland. All samples were tested using a previously described multiplex real-time PCR for the Anaplasmataceae family, the 'Candidatus Neoehrlichia' genus and the 'Candidatus Neoehrlichia mikurensis' species. All Anaplasmataceae positive samples were subsequently tested using specific real-time PCRs for Anaplasma phagocytophilum, Anaplasma platys, Ehrlichia canis and Rickettsia helvetica. Among the tested animals, one dog from Zurich tested positive for 'Candidatus Neoehrlichia mikurensis'. The 12-year old West Highland white terrier had been splenectomized 3 months prior to the blood collection and presented with polyuria/polydipsia. Fanconi syndrome was diagnosed based on glucosuria with normoglycemia and hyperaminoaciduria. A. platys and E. canis were detected in 14/249 dogs from Sicily and Portugal; two of the dogs were coinfected with both agents. Four Swiss foxes tested positive for A. phagocytophilium. R. helvetica was detected for the first time in a red fox. In conclusion, 'Candidatus Neoehrlichia mikurensis' infection should be considered in sick dogs, particularly when immunocompromised. The pathogen seems not to be widespread in Canidae in the investigated countries. Conversely, other Anaplasmataceae were more readily detected in dogs and foxes.

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Authors+Show Affiliations

Hofmann-Lehmann R
Clinical Laboratory, Vetsuisse Faculty, University of Zurich, Switzerland. Center for Clinical Studies, Vetsuisse Faculty, University of Zurich, Switzerland.
Wagmann N
Clinical Laboratory, Vetsuisse Faculty, University of Zurich, Switzerland. Center for Clinical Studies, Vetsuisse Faculty, University of Zurich, Switzerland.
Meli ML
Clinical Laboratory, Vetsuisse Faculty, University of Zurich, Switzerland. Center for Clinical Studies, Vetsuisse Faculty, University of Zurich, Switzerland.
Riond B
Clinical Laboratory, Vetsuisse Faculty, University of Zurich, Switzerland.
Novacco M
Clinical Laboratory, Vetsuisse Faculty, University of Zurich, Switzerland. Center for Clinical Studies, Vetsuisse Faculty, University of Zurich, Switzerland.
Joekel D
Institute of Parasitology, Vetsuisse Faculty, University of Zurich, Switzerland.
Gentilini F
Veterinary Clinical Department, Faculty of Veterinary Medicine, University of Bologna, Italy.
Marsilio F
Faculty of Veterinary Medicine, University of Teramo, Località Piano d'Accio, Teramo, Italy.
Pennisi MG
Department of Veterinary Science, University of Messina, Polo Universitario dell'Annunziata, Messina, Italy.
Lloret A
Veterinary Teaching Hospital, Faculty of Veterinary Medicine, Autonomous University of Barcelona, Bellaterra, Spain.
Carrapiço T
Clinica Veterinaria Canham, Avenida Duarte Pacheco, Almancil, Portugal.
Boretti FS
Clinic for Small Animal Internal Medicine, Department for Small Animals, Vetsuisse Faculty, University of Zurich.

MeSH

AnaplasmataceaeAnaplasmataceae InfectionsAnimalsCoinfectionDog DiseasesDogsFoxesGenes, BacterialMediterranean RegionPolymerase Chain ReactionPrevalenceRickettsiaceaeRickettsiaceae InfectionsSwitzerlandZoonoses

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27707682

Citation

Hofmann-Lehmann, R, et al. "Detection of 'Candidatus Neoehrlichia Mikurensis' and Other Anaplasmataceae and Rickettsiaceae in Canidae in Switzerland and Mediterranean Countries." Schweizer Archiv Fur Tierheilkunde, vol. 158, no. 10, 2016, pp. 691-700.
Hofmann-Lehmann R, Wagmann N, Meli ML, et al. Detection of 'Candidatus Neoehrlichia mikurensis' and other Anaplasmataceae and Rickettsiaceae in Canidae in Switzerland and Mediterranean countries. Schweiz Arch Tierheilkd. 2016;158(10):691-700.
Hofmann-Lehmann, R., Wagmann, N., Meli, M. L., Riond, B., Novacco, M., Joekel, D., Gentilini, F., Marsilio, F., Pennisi, M. G., Lloret, A., Carrapiço, T., & Boretti, F. S. (2016). Detection of 'Candidatus Neoehrlichia mikurensis' and other Anaplasmataceae and Rickettsiaceae in Canidae in Switzerland and Mediterranean countries. Schweizer Archiv Fur Tierheilkunde, 158(10), 691-700. https://doi.org/10.17236/sat00087
Hofmann-Lehmann R, et al. Detection of 'Candidatus Neoehrlichia Mikurensis' and Other Anaplasmataceae and Rickettsiaceae in Canidae in Switzerland and Mediterranean Countries. Schweiz Arch Tierheilkd. 2016;158(10):691-700. PubMed PMID: 27707682.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of 'Candidatus Neoehrlichia mikurensis' and other Anaplasmataceae and Rickettsiaceae in Canidae in Switzerland and Mediterranean countries. AU - Hofmann-Lehmann,R, AU - Wagmann,N, AU - Meli,M L, AU - Riond,B, AU - Novacco,M, AU - Joekel,D, AU - Gentilini,F, AU - Marsilio,F, AU - Pennisi,M G, AU - Lloret,A, AU - Carrapiço,T, AU - Boretti,F S, PY - 2016/10/7/entrez PY - 2016/10/7/pubmed PY - 2016/12/17/medline KW - Anaplasmataceae KW - Canidae KW - Kaniden KW - Rickettsia helvetica KW - Zoonose KW - zoonosis KW - ‘Candidatus Neoehrlichia mikurensis’ SP - 691 EP - 700 JF - Schweizer Archiv fur Tierheilkunde JO - Schweiz Arch Tierheilkd VL - 158 IS - 10 N2 - INTRODUCTION: 'Candidatus Neoehrlichia mikurensis' is an emerging tick-borne zoonotic agent that primarily affects immunocompromised human patients. Dogs and foxes are frequently exposed to ticks, and both species are in close proximity to humans. This is the first study to systematically investigate the occurrence of 'Candidatus Neoehrlichia mikurensis' in Canidae in Europa. We analyzed 1'739 blood samples from dogs in Switzerland, Italy, Spain and Portugal and 162 blood samples from free-ranging red foxes (Vulpes vulpes) in Switzerland. All samples were tested using a previously described multiplex real-time PCR for the Anaplasmataceae family, the 'Candidatus Neoehrlichia' genus and the 'Candidatus Neoehrlichia mikurensis' species. All Anaplasmataceae positive samples were subsequently tested using specific real-time PCRs for Anaplasma phagocytophilum, Anaplasma platys, Ehrlichia canis and Rickettsia helvetica. Among the tested animals, one dog from Zurich tested positive for 'Candidatus Neoehrlichia mikurensis'. The 12-year old West Highland white terrier had been splenectomized 3 months prior to the blood collection and presented with polyuria/polydipsia. Fanconi syndrome was diagnosed based on glucosuria with normoglycemia and hyperaminoaciduria. A. platys and E. canis were detected in 14/249 dogs from Sicily and Portugal; two of the dogs were coinfected with both agents. Four Swiss foxes tested positive for A. phagocytophilium. R. helvetica was detected for the first time in a red fox. In conclusion, 'Candidatus Neoehrlichia mikurensis' infection should be considered in sick dogs, particularly when immunocompromised. The pathogen seems not to be widespread in Canidae in the investigated countries. Conversely, other Anaplasmataceae were more readily detected in dogs and foxes. SN - 0036-7281 UR - https://www.unboundmedicine.com/medline/citation/27707682/Detection_of_'Candidatus_Neoehrlichia_mikurensis'_and_other_Anaplasmataceae_and_Rickettsiaceae_in_Canidae_in_Switzerland_and_Mediterranean_countries_ DB - PRIME DP - Unbound Medicine ER -