Design and Validation of CRISPR/Cas9 Systems for Targeted Gene Modification in Induced Pluripotent Stem Cells.Methods Mol Biol. 2017; 1498:3-21.MM
Abstract
The CRISPR/Cas9 system is a powerful tool for precision genome editing. The ability to accurately modify genomic DNA in situ with single nucleotide precision opens up new possibilities for not only basic research but also biotechnology applications and clinical translation. In this chapter, we outline the procedures for design, screening, and validation of CRISPR/Cas9 systems for targeted modification of coding sequences in the human genome and how to perform genome editing in induced pluripotent stem cells with high efficiency and specificity.
Links
MeSH
Pub Type(s)
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Language
eng
PubMed ID
27709565
Citation
Lee, Ciaran M., et al. "Design and Validation of CRISPR/Cas9 Systems for Targeted Gene Modification in Induced Pluripotent Stem Cells." Methods in Molecular Biology (Clifton, N.J.), vol. 1498, 2017, pp. 3-21.
Lee CM, Zhu H, Davis TH, et al. Design and Validation of CRISPR/Cas9 Systems for Targeted Gene Modification in Induced Pluripotent Stem Cells. Methods Mol Biol. 2017;1498:3-21.
Lee, C. M., Zhu, H., Davis, T. H., Deshmukh, H., & Bao, G. (2017). Design and Validation of CRISPR/Cas9 Systems for Targeted Gene Modification in Induced Pluripotent Stem Cells. Methods in Molecular Biology (Clifton, N.J.), 1498, 3-21.
Lee CM, et al. Design and Validation of CRISPR/Cas9 Systems for Targeted Gene Modification in Induced Pluripotent Stem Cells. Methods Mol Biol. 2017;1498:3-21. PubMed PMID: 27709565.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR
T1 - Design and Validation of CRISPR/Cas9 Systems for Targeted Gene Modification in Induced Pluripotent Stem Cells.
AU - Lee,Ciaran M,
AU - Zhu,Haibao,
AU - Davis,Timothy H,
AU - Deshmukh,Harshahardhan,
AU - Bao,Gang,
PY - 2016/10/7/entrez
PY - 2016/10/7/pubmed
PY - 2018/1/6/medline
KW - CRISPR
KW - Genome editing
KW - Targeted gene knockout
SP - 3
EP - 21
JF - Methods in molecular biology (Clifton, N.J.)
JO - Methods Mol Biol
VL - 1498
N2 - The CRISPR/Cas9 system is a powerful tool for precision genome editing. The ability to accurately modify genomic DNA in situ with single nucleotide precision opens up new possibilities for not only basic research but also biotechnology applications and clinical translation. In this chapter, we outline the procedures for design, screening, and validation of CRISPR/Cas9 systems for targeted modification of coding sequences in the human genome and how to perform genome editing in induced pluripotent stem cells with high efficiency and specificity.
SN - 1940-6029
UR - https://www.unboundmedicine.com/medline/citation/27709565/Design_and_Validation_of_CRISPR/Cas9_Systems_for_Targeted_Gene_Modification_in_Induced_Pluripotent_Stem_Cells_
L2 - https://dx.doi.org/10.1007/978-1-4939-6472-7_1
DB - PRIME
DP - Unbound Medicine
ER -