Tags

Type your tag names separated by a space and hit enter

Design and Validation of CRISPR/Cas9 Systems for Targeted Gene Modification in Induced Pluripotent Stem Cells.
Methods Mol Biol. 2017; 1498:3-21.MM

Abstract

The CRISPR/Cas9 system is a powerful tool for precision genome editing. The ability to accurately modify genomic DNA in situ with single nucleotide precision opens up new possibilities for not only basic research but also biotechnology applications and clinical translation. In this chapter, we outline the procedures for design, screening, and validation of CRISPR/Cas9 systems for targeted modification of coding sequences in the human genome and how to perform genome editing in induced pluripotent stem cells with high efficiency and specificity.

Links

Publisher Full Text

Authors+Show Affiliations

Lee CM
Department of Bioengineering, Rice University, 6500 Main St., Houston, TX, 77030, USA.
Zhu H
Department of Bioengineering, Rice University, 6500 Main St., Houston, TX, 77030, USA.
Davis TH
Department of Bioengineering, Rice University, 6500 Main St., Houston, TX, 77030, USA.
Deshmukh H
Department of Bioengineering, Rice University, 6500 Main St., Houston, TX, 77030, USA.
Bao G
Department of Bioengineering, Rice University, 6500 Main St., Houston, TX, 77030, USA. gang.bao@rice.edu.

MeSH

CRISPR-Cas SystemsCell LineClustered Regularly Interspaced Short Palindromic RepeatsGene EditingGenome, HumanHEK293 CellsHumansPluripotent Stem CellsRNA Editing

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27709565

Citation

Lee, Ciaran M., et al. "Design and Validation of CRISPR/Cas9 Systems for Targeted Gene Modification in Induced Pluripotent Stem Cells." Methods in Molecular Biology (Clifton, N.J.), vol. 1498, 2017, pp. 3-21.
Lee CM, Zhu H, Davis TH, et al. Design and Validation of CRISPR/Cas9 Systems for Targeted Gene Modification in Induced Pluripotent Stem Cells. Methods Mol Biol. 2017;1498:3-21.
Lee, C. M., Zhu, H., Davis, T. H., Deshmukh, H., & Bao, G. (2017). Design and Validation of CRISPR/Cas9 Systems for Targeted Gene Modification in Induced Pluripotent Stem Cells. Methods in Molecular Biology (Clifton, N.J.), 1498, 3-21.
Lee CM, et al. Design and Validation of CRISPR/Cas9 Systems for Targeted Gene Modification in Induced Pluripotent Stem Cells. Methods Mol Biol. 2017;1498:3-21. PubMed PMID: 27709565.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Design and Validation of CRISPR/Cas9 Systems for Targeted Gene Modification in Induced Pluripotent Stem Cells. AU - Lee,Ciaran M, AU - Zhu,Haibao, AU - Davis,Timothy H, AU - Deshmukh,Harshahardhan, AU - Bao,Gang, PY - 2016/10/7/entrez PY - 2016/10/7/pubmed PY - 2018/1/6/medline KW - CRISPR KW - Genome editing KW - Targeted gene knockout SP - 3 EP - 21 JF - Methods in molecular biology (Clifton, N.J.) JO - Methods Mol Biol VL - 1498 N2 - The CRISPR/Cas9 system is a powerful tool for precision genome editing. The ability to accurately modify genomic DNA in situ with single nucleotide precision opens up new possibilities for not only basic research but also biotechnology applications and clinical translation. In this chapter, we outline the procedures for design, screening, and validation of CRISPR/Cas9 systems for targeted modification of coding sequences in the human genome and how to perform genome editing in induced pluripotent stem cells with high efficiency and specificity. SN - 1940-6029 UR - https://www.unboundmedicine.com/medline/citation/27709565/Design_and_Validation_of_CRISPR/Cas9_Systems_for_Targeted_Gene_Modification_in_Induced_Pluripotent_Stem_Cells_ L2 - https://dx.doi.org/10.1007/978-1-4939-6472-7_1 DB - PRIME DP - Unbound Medicine ER -