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Use of CRISPR/Cas Genome Editing Technology for Targeted Mutagenesis in Rice.
Methods Mol Biol. 2017; 1498:33-40.MM

Abstract

Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated protein (Cas) system is a newly emerging mutagenesis (gene-editing) tool in genetic engineering. Among the agriculturally important crops, several genes have been successfully mutated by the system, and some agronomic important traits have been rapidly generated, which indicates the potential applications in both scientific research and plant breeding. In this chapter, we describe a standard gene-editing procedure to effectively target rice genes and to make specific rice mutants using the CRISPR/Cas9 system mediated by Agrobacterium transformation.

Authors+Show Affiliations

Key Laboratory of Rice Genetics Breeding of Anhui Province, Rice Research Institute, Anhui Academy of Agricultural Sciences, 40# Nongke South Road, Hefei, 230031, China.Key Laboratory of Rice Genetics Breeding of Anhui Province, Rice Research Institute, Anhui Academy of Agricultural Sciences, 40# Nongke South Road, Hefei, 230031, China. weipengcheng@gmail.com.Key Laboratory of Rice Genetics Breeding of Anhui Province, Rice Research Institute, Anhui Academy of Agricultural Sciences, 40# Nongke South Road, Hefei, 230031, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27709567

Citation

Xu, Rongfang, et al. "Use of CRISPR/Cas Genome Editing Technology for Targeted Mutagenesis in Rice." Methods in Molecular Biology (Clifton, N.J.), vol. 1498, 2017, pp. 33-40.
Xu R, Wei P, Yang J. Use of CRISPR/Cas Genome Editing Technology for Targeted Mutagenesis in Rice. Methods Mol Biol. 2017;1498:33-40.
Xu, R., Wei, P., & Yang, J. (2017). Use of CRISPR/Cas Genome Editing Technology for Targeted Mutagenesis in Rice. Methods in Molecular Biology (Clifton, N.J.), 1498, 33-40.
Xu R, Wei P, Yang J. Use of CRISPR/Cas Genome Editing Technology for Targeted Mutagenesis in Rice. Methods Mol Biol. 2017;1498:33-40. PubMed PMID: 27709567.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Use of CRISPR/Cas Genome Editing Technology for Targeted Mutagenesis in Rice. AU - Xu,Rongfang, AU - Wei,Pengcheng, AU - Yang,Jianbo, PY - 2016/10/7/entrez PY - 2016/10/7/pubmed PY - 2018/1/6/medline KW - Agrobacterium transformation KW - CRISPR/Cas9 KW - Mutagenesis KW - Rice SP - 33 EP - 40 JF - Methods in molecular biology (Clifton, N.J.) JO - Methods Mol Biol VL - 1498 N2 - Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated protein (Cas) system is a newly emerging mutagenesis (gene-editing) tool in genetic engineering. Among the agriculturally important crops, several genes have been successfully mutated by the system, and some agronomic important traits have been rapidly generated, which indicates the potential applications in both scientific research and plant breeding. In this chapter, we describe a standard gene-editing procedure to effectively target rice genes and to make specific rice mutants using the CRISPR/Cas9 system mediated by Agrobacterium transformation. SN - 1940-6029 UR - https://www.unboundmedicine.com/medline/citation/27709567/Use_of_CRISPR/Cas_Genome_Editing_Technology_for_Targeted_Mutagenesis_in_Rice_ L2 - https://dx.doi.org/10.1007/978-1-4939-6472-7_3 DB - PRIME DP - Unbound Medicine ER -