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Protective effects of seed melon extract on CCl4-induced hepatic fibrosis in mice.
J Ethnopharmacol. 2016 Dec 04; 193:531-537.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Citrullus lanatus ssp. vulgaris var. megalaspermus Lin et Chao, was also known as watermelon belongs to family Cucurbitaceae, variously used as healthy food and in the treatment of liver and lungs problems. Currently, Citrullus lanatus has become a major economic crop of medicinal and edible effects with regional characteristics.

AIM

This study was designed to evaluate the hepatoprotective and antioxidant activity of the seed melon (Citrullus lanatus ssp. vulgaris var. megalaspermus Lin et Chao) extract (SME) against carbon tetrachloride (CCl4) induced hepatic fibrosis in mice.

MATERIALS AND METHODS

In this study, mice were randomly divided into 7 groups, including normal control, model, silymarin tablets as the positive control, SME 100, 200, 400, and 800mg/kg. After 8 weeks, activities of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglycerides (TG), hyaluronic acid (HA) and laminin (LN) were checked. The levels of antioxidant enzymes such as superoxide dismutase (SOD), glutataion (GSH) and glutathione peroxidase (GSH-Px) were determined after SME administration. The hydroxyproline (HYP) levels, malondialdehyde (MDA) levels and histopathologic examinations of hepatocyte fibrosis were also determined. Additionally, effects of SME on alpha-smooth muscle actin (α-SMA) and transforming growth factor beta-1(TGF-β1) protein expressions were determined.

RESULTS

We found that SME could significantly lower the serum levels of hepatic enzyme markers AST, ALT, HA and LN (P<0.01). Compared with the CCl4-only treatment group, levels of hepatic SOD and GSH-Px were significantly increased, and the MDA levels were remarkably decreased in mice treated by SME at medium dose (400mg/kg) and high dose (800mg/kg) (P<0.01). A histological examination of the liver showed that lesions, including necrosis, lymphocyte infiltration and fatty degeneration, were partially healed by treatment with SME. The results of protein expressions studies displayed that SME could inhibit α-SMA and TGF-β1 protein expression (P<0.01).

CONCLUSION

The present results suggested that protective effect of SME against CCl4-induced hepatic fibrosis may rely on its effect on reducing oxidative stress and improving drug metabolizing enzyme activity in liver.

Authors+Show Affiliations

Key Laboratory of Xinjiang Phytomedicine Resources of Ministry of Education, School of Pharmacy, Shihezi University, Shihezi 832000, China.Key Laboratory of Xinjiang Phytomedicine Resources of Ministry of Education, School of Pharmacy, Shihezi University, Shihezi 832000, China; Shenyang Pharmaceutical University, Shenyang 110016, China.Key Laboratory of Xinjiang Phytomedicine Resources of Ministry of Education, School of Pharmacy, Shihezi University, Shihezi 832000, China.Shenzhen Fairy Lake Botanical Garden of Chinese Academy of Sciences, Shenzhen 518004, China.Shihezi Vegetable Research Institute, Shihezi 832000, China.Key Laboratory of Xinjiang Phytomedicine Resources of Ministry of Education, School of Pharmacy, Shihezi University, Shihezi 832000, China. Electronic address: 13999333713@163.com.

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

27717904

Citation

Zhan, Yuan-Yuan, et al. "Protective Effects of Seed Melon Extract On CCl4-induced Hepatic Fibrosis in Mice." Journal of Ethnopharmacology, vol. 193, 2016, pp. 531-537.
Zhan YY, Wang JH, Tian X, et al. Protective effects of seed melon extract on CCl4-induced hepatic fibrosis in mice. J Ethnopharmacol. 2016;193:531-537.
Zhan, Y. Y., Wang, J. H., Tian, X., Feng, S. X., Xue, L., & Tian, L. P. (2016). Protective effects of seed melon extract on CCl4-induced hepatic fibrosis in mice. Journal of Ethnopharmacology, 193, 531-537. https://doi.org/10.1016/j.jep.2016.10.006
Zhan YY, et al. Protective Effects of Seed Melon Extract On CCl4-induced Hepatic Fibrosis in Mice. J Ethnopharmacol. 2016 Dec 4;193:531-537. PubMed PMID: 27717904.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective effects of seed melon extract on CCl4-induced hepatic fibrosis in mice. AU - Zhan,Yuan-Yuan, AU - Wang,Jin-Hui, AU - Tian,Xing, AU - Feng,Shi-Xiu, AU - Xue,Lin, AU - Tian,Li-Ping, Y1 - 2016/10/04/ PY - 2016/03/21/received PY - 2016/09/04/revised PY - 2016/10/03/accepted PY - 2016/10/9/pubmed PY - 2017/4/18/medline PY - 2016/10/9/entrez KW - Carbon tetrachloride KW - Citrullus lanatus KW - Hepatic fibrosis KW - Silymarin KW - Western blot SP - 531 EP - 537 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 193 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Citrullus lanatus ssp. vulgaris var. megalaspermus Lin et Chao, was also known as watermelon belongs to family Cucurbitaceae, variously used as healthy food and in the treatment of liver and lungs problems. Currently, Citrullus lanatus has become a major economic crop of medicinal and edible effects with regional characteristics. AIM: This study was designed to evaluate the hepatoprotective and antioxidant activity of the seed melon (Citrullus lanatus ssp. vulgaris var. megalaspermus Lin et Chao) extract (SME) against carbon tetrachloride (CCl4) induced hepatic fibrosis in mice. MATERIALS AND METHODS: In this study, mice were randomly divided into 7 groups, including normal control, model, silymarin tablets as the positive control, SME 100, 200, 400, and 800mg/kg. After 8 weeks, activities of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglycerides (TG), hyaluronic acid (HA) and laminin (LN) were checked. The levels of antioxidant enzymes such as superoxide dismutase (SOD), glutataion (GSH) and glutathione peroxidase (GSH-Px) were determined after SME administration. The hydroxyproline (HYP) levels, malondialdehyde (MDA) levels and histopathologic examinations of hepatocyte fibrosis were also determined. Additionally, effects of SME on alpha-smooth muscle actin (α-SMA) and transforming growth factor beta-1(TGF-β1) protein expressions were determined. RESULTS: We found that SME could significantly lower the serum levels of hepatic enzyme markers AST, ALT, HA and LN (P<0.01). Compared with the CCl4-only treatment group, levels of hepatic SOD and GSH-Px were significantly increased, and the MDA levels were remarkably decreased in mice treated by SME at medium dose (400mg/kg) and high dose (800mg/kg) (P<0.01). A histological examination of the liver showed that lesions, including necrosis, lymphocyte infiltration and fatty degeneration, were partially healed by treatment with SME. The results of protein expressions studies displayed that SME could inhibit α-SMA and TGF-β1 protein expression (P<0.01). CONCLUSION: The present results suggested that protective effect of SME against CCl4-induced hepatic fibrosis may rely on its effect on reducing oxidative stress and improving drug metabolizing enzyme activity in liver. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/27717904/Protective_effects_of_seed_melon_extract_on_CCl4_induced_hepatic_fibrosis_in_mice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(16)31131-X DB - PRIME DP - Unbound Medicine ER -