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Development of a new polyspecific antivenom for snakebite envenoming in Sri Lanka: Analysis of its preclinical efficacy as compared to a currently available antivenom.
Toxicon. 2016 Nov; 122:152-159.T

Abstract

A new whole IgG, freeze-dried, polyspecific antivenom was prepared from the plasma of horses immunized with the venoms of the snakes Daboia russelii, Echis carinatus, Hypnale hypnale, and Naja naja from Sri Lanka. The preclinical neutralizing ability of this antivenom against several toxic and enzymatic activities of these four venoms was analyzed, and compared with that of a batch of VINS antivenom (India) being currently used in Sri Lanka. The activities tested were: lethality, hemorrhagic, in vitro coagulant, proteinase and phospholipase A2. Both antivenoms neutralized, to a different extent, these activities of the venom of D. russelii, E. carinatus, and N. naja. In general, the polyspecific Sri Lankan antivenom was more effective than the Indian antivenom in the neutralization of the venoms of D. russelii and E. carinatus, whereas the Indian antivenom showed a higher efficacy against the venom of N. naja. Regarding H. hypnale, the new Sri Lankan antivenom was effective in the neutralization of all activities tested, whereas the Indian antivenom neutralized lethality but not hemorrhagic, coagulant, proteinase and PLA2 activities, in agreement with the fact that this venom is not included in the immunization mixture for this antivenom. Results suggest that the new polyspecific Sri Lankan antivenom has a satisfactory preclinical neutralizing profile and compares favorably with the Indian antivenom. This is ready to be tested in a clinical trial to evaluate its efficacy and safety in human victims of snakebite envenomings by D. russelii, E. carinatus and H. hypnale in Sri Lanka.

Authors+Show Affiliations

Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica.Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica.Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica.Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica.Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica.Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica.Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica.Faculty of Medicine, University of Peradeniya, Sri Lanka.Animal Venom Research International, Moreno Valley, CA, USA; Department of Experimental and Clinical Pharmacology, University of Minnesota, Minneapolis, MN, USA.Animal Venom Research International, Moreno Valley, CA, USA.Animal Venom Research International, Moreno Valley, CA, USA.Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica.Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica.Instituto Clodomiro Picado, Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica. Electronic address: jose.gutierrez@ucr.ac.cr.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27720977

Citation

Villalta, Mauren, et al. "Development of a New Polyspecific Antivenom for Snakebite Envenoming in Sri Lanka: Analysis of Its Preclinical Efficacy as Compared to a Currently Available Antivenom." Toxicon : Official Journal of the International Society On Toxinology, vol. 122, 2016, pp. 152-159.
Villalta M, Sánchez A, Herrera M, et al. Development of a new polyspecific antivenom for snakebite envenoming in Sri Lanka: Analysis of its preclinical efficacy as compared to a currently available antivenom. Toxicon. 2016;122:152-159.
Villalta, M., Sánchez, A., Herrera, M., Vargas, M., Segura, Á., Cerdas, M., Estrada, R., Gawarammana, I., Keyler, D. E., McWhorter, K., Malleappah, R., Alape-Girón, A., León, G., & Gutiérrez, J. M. (2016). Development of a new polyspecific antivenom for snakebite envenoming in Sri Lanka: Analysis of its preclinical efficacy as compared to a currently available antivenom. Toxicon : Official Journal of the International Society On Toxinology, 122, 152-159. https://doi.org/10.1016/j.toxicon.2016.10.007
Villalta M, et al. Development of a New Polyspecific Antivenom for Snakebite Envenoming in Sri Lanka: Analysis of Its Preclinical Efficacy as Compared to a Currently Available Antivenom. Toxicon. 2016;122:152-159. PubMed PMID: 27720977.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development of a new polyspecific antivenom for snakebite envenoming in Sri Lanka: Analysis of its preclinical efficacy as compared to a currently available antivenom. AU - Villalta,Mauren, AU - Sánchez,Andrés, AU - Herrera,María, AU - Vargas,Mariángela, AU - Segura,Álvaro, AU - Cerdas,Maykel, AU - Estrada,Ricardo, AU - Gawarammana,Indika, AU - Keyler,Dan E, AU - McWhorter,Kimberly, AU - Malleappah,Roy, AU - Alape-Girón,Alberto, AU - León,Guillermo, AU - Gutiérrez,José María, Y1 - 2016/10/06/ PY - 2016/06/27/received PY - 2016/08/26/revised PY - 2016/10/05/accepted PY - 2016/10/30/pubmed PY - 2017/4/22/medline PY - 2016/10/11/entrez KW - Antivenoms KW - Daboia russelii KW - Echis carinatus KW - Hypnale hypnale KW - Naja naja KW - Neutralization KW - Snake venoms KW - Sri Lanka SP - 152 EP - 159 JF - Toxicon : official journal of the International Society on Toxinology JO - Toxicon VL - 122 N2 - A new whole IgG, freeze-dried, polyspecific antivenom was prepared from the plasma of horses immunized with the venoms of the snakes Daboia russelii, Echis carinatus, Hypnale hypnale, and Naja naja from Sri Lanka. The preclinical neutralizing ability of this antivenom against several toxic and enzymatic activities of these four venoms was analyzed, and compared with that of a batch of VINS antivenom (India) being currently used in Sri Lanka. The activities tested were: lethality, hemorrhagic, in vitro coagulant, proteinase and phospholipase A2. Both antivenoms neutralized, to a different extent, these activities of the venom of D. russelii, E. carinatus, and N. naja. In general, the polyspecific Sri Lankan antivenom was more effective than the Indian antivenom in the neutralization of the venoms of D. russelii and E. carinatus, whereas the Indian antivenom showed a higher efficacy against the venom of N. naja. Regarding H. hypnale, the new Sri Lankan antivenom was effective in the neutralization of all activities tested, whereas the Indian antivenom neutralized lethality but not hemorrhagic, coagulant, proteinase and PLA2 activities, in agreement with the fact that this venom is not included in the immunization mixture for this antivenom. Results suggest that the new polyspecific Sri Lankan antivenom has a satisfactory preclinical neutralizing profile and compares favorably with the Indian antivenom. This is ready to be tested in a clinical trial to evaluate its efficacy and safety in human victims of snakebite envenomings by D. russelii, E. carinatus and H. hypnale in Sri Lanka. SN - 1879-3150 UR - https://www.unboundmedicine.com/medline/citation/27720977/Development_of_a_new_polyspecific_antivenom_for_snakebite_envenoming_in_Sri_Lanka:_Analysis_of_its_preclinical_efficacy_as_compared_to_a_currently_available_antivenom_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0041-0101(16)30300-2 DB - PRIME DP - Unbound Medicine ER -