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Preparation of monoclonal antibody against Angiostrongylus cantonensis antigen.
Southeast Asian J Trop Med Public Health. 1989 Mar; 20(1):119-24.SA

Abstract

Monoclonal antibodies (MAb) against A cantonensis were produced through fusion of immunised spleen cells from BALB/c mice with NS-1 myeloma cells at a ratio of 10:1. The successful fusion rate on the 3rd day of fusion was 90.1%. Ten MAb were characterised, six of which were IgG1 and the remaining four were IgG2a, IgG2b, IgM and IgA respectively. Among 6 IgG1 MAb, four were A. cantonensis-specific, of which three reacted to adult worm antigen only and one reacted to both adult worm and juvenile worm antigens. Two other IgG1 MAb showed cross-reaction with other helminthic antigens of Toxocara canis. Ascaris suum. Paragonimus westermani, Dirofilaria immitis, Anisakis Spp, Gnatostoma Spinigerum and Clonorchis sinensis.

Authors+Show Affiliations

Department of Parasitology, Kaohsiung Medical College, Taiwan, R.O.C.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

2772697

Citation

Yen, C M., et al. "Preparation of Monoclonal Antibody Against Angiostrongylus Cantonensis Antigen." The Southeast Asian Journal of Tropical Medicine and Public Health, vol. 20, no. 1, 1989, pp. 119-24.
Yen CM, Chen ER, Kojima S, et al. Preparation of monoclonal antibody against Angiostrongylus cantonensis antigen. Southeast Asian J Trop Med Public Health. 1989;20(1):119-24.
Yen, C. M., Chen, E. R., Kojima, S., & Kobayashi, M. (1989). Preparation of monoclonal antibody against Angiostrongylus cantonensis antigen. The Southeast Asian Journal of Tropical Medicine and Public Health, 20(1), 119-24.
Yen CM, et al. Preparation of Monoclonal Antibody Against Angiostrongylus Cantonensis Antigen. Southeast Asian J Trop Med Public Health. 1989;20(1):119-24. PubMed PMID: 2772697.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Preparation of monoclonal antibody against Angiostrongylus cantonensis antigen. AU - Yen,C M, AU - Chen,E R, AU - Kojima,S, AU - Kobayashi,M, PY - 1989/3/1/pubmed PY - 1989/3/1/medline PY - 1989/3/1/entrez SP - 119 EP - 24 JF - The Southeast Asian journal of tropical medicine and public health JO - Southeast Asian J Trop Med Public Health VL - 20 IS - 1 N2 - Monoclonal antibodies (MAb) against A cantonensis were produced through fusion of immunised spleen cells from BALB/c mice with NS-1 myeloma cells at a ratio of 10:1. The successful fusion rate on the 3rd day of fusion was 90.1%. Ten MAb were characterised, six of which were IgG1 and the remaining four were IgG2a, IgG2b, IgM and IgA respectively. Among 6 IgG1 MAb, four were A. cantonensis-specific, of which three reacted to adult worm antigen only and one reacted to both adult worm and juvenile worm antigens. Two other IgG1 MAb showed cross-reaction with other helminthic antigens of Toxocara canis. Ascaris suum. Paragonimus westermani, Dirofilaria immitis, Anisakis Spp, Gnatostoma Spinigerum and Clonorchis sinensis. SN - 0125-1562 UR - https://www.unboundmedicine.com/medline/citation/2772697/Preparation_of_monoclonal_antibody_against_Angiostrongylus_cantonensis_antigen_ DB - PRIME DP - Unbound Medicine ER -