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Coexistence of free-living amoebae and bacteria in selected South African hospital water distribution systems.
Parasitol Res. 2017 Jan; 116(1):155-165.PR

Abstract

Pathogenic free-living amoebae (FLA), such as Naegleria fowleri, Balamuthia mandrillaris and Acanthamoeba species isolated from aquatic environments have been implicated in central nervous system, eye and skin human infections. They also allow the survival, growth and transmission of bacteria such as Legionella, Mycobacteria and Vibrio species in water systems. The purpose of this study was to investigate the co-occurrence of potentially pathogenic FLA and their associated bacteria in hospital water networks in Johannesburg, South Africa. A total of 178 water (n = 95) and swab (n = 83) samples were collected from two hospital water distribution systems. FLA were isolated using the amoebal enrichment technique and identified using PCR and 18S rDNA sequencing. Amoebae potentially containing intra-amoebal bacteria were lysed and cultured on blood agar plates. Bacterial isolates were characterized using the VITEK®2 compact System. Free-living amoebae were isolated from 77 (43.3 %) of the samples. Using microscopy, PCR and 18S rRNA sequencing, Acanthamoeba spp. (T3 and T20 genotypes), Vermamoeba vermiformis and Naegleria gruberi specie were identified. The Acanthamoeba T3 and T20 genotypes have been implicated in eye and central nervous system infections. The most commonly detected bacterial species were Serratia marcescens, Stenotrophomonas maltophilia, Delftia acidovorans, Sphingomonas paucimobilis and Comamonas testosteroni. These nosocomial pathogenic bacteria are associated with systematic blood, respiratory tract, the urinary tract, surgical wounds and soft tissues infections. The detection of FLA and their associated opportunistic bacteria in the hospital water systems point out to a potential health risk to immune-compromised individuals.

Authors+Show Affiliations

Water and Health Research Centre, Faculty of Health Sciences, University of Johannesburg, PO Box 17011, Doornfontein, 2028, South Africa. chesap@gmail.com.Department of Hygiene, Social and Environmental Medicine, Ruhr-University Bochum, Universitätsstraβe 150, 44801, Bochum, Germany.Department of Hygiene, Social and Environmental Medicine, Ruhr-University Bochum, Universitätsstraβe 150, 44801, Bochum, Germany.Water and Health Research Centre, Faculty of Health Sciences, University of Johannesburg, PO Box 17011, Doornfontein, 2028, South Africa.Water and Health Research Centre, Faculty of Health Sciences, University of Johannesburg, PO Box 17011, Doornfontein, 2028, South Africa.Water and Health Research Centre, Faculty of Health Sciences, University of Johannesburg, PO Box 17011, Doornfontein, 2028, South Africa.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27730363

Citation

Muchesa, P, et al. "Coexistence of Free-living Amoebae and Bacteria in Selected South African Hospital Water Distribution Systems." Parasitology Research, vol. 116, no. 1, 2017, pp. 155-165.
Muchesa P, Leifels M, Jurzik L, et al. Coexistence of free-living amoebae and bacteria in selected South African hospital water distribution systems. Parasitol Res. 2017;116(1):155-165.
Muchesa, P., Leifels, M., Jurzik, L., Hoorzook, K. B., Barnard, T. G., & Bartie, C. (2017). Coexistence of free-living amoebae and bacteria in selected South African hospital water distribution systems. Parasitology Research, 116(1), 155-165.
Muchesa P, et al. Coexistence of Free-living Amoebae and Bacteria in Selected South African Hospital Water Distribution Systems. Parasitol Res. 2017;116(1):155-165. PubMed PMID: 27730363.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Coexistence of free-living amoebae and bacteria in selected South African hospital water distribution systems. AU - Muchesa,P, AU - Leifels,M, AU - Jurzik,L, AU - Hoorzook,K B, AU - Barnard,T G, AU - Bartie,C, Y1 - 2016/10/12/ PY - 2016/06/20/received PY - 2016/09/21/accepted PY - 2016/10/13/pubmed PY - 2017/6/6/medline PY - 2016/10/13/entrez KW - Acanthamoeba spp. KW - Amoebal enrichment KW - Serretia marcescens KW - Vermamoeba vermiformis SP - 155 EP - 165 JF - Parasitology research JO - Parasitol Res VL - 116 IS - 1 N2 - Pathogenic free-living amoebae (FLA), such as Naegleria fowleri, Balamuthia mandrillaris and Acanthamoeba species isolated from aquatic environments have been implicated in central nervous system, eye and skin human infections. They also allow the survival, growth and transmission of bacteria such as Legionella, Mycobacteria and Vibrio species in water systems. The purpose of this study was to investigate the co-occurrence of potentially pathogenic FLA and their associated bacteria in hospital water networks in Johannesburg, South Africa. A total of 178 water (n = 95) and swab (n = 83) samples were collected from two hospital water distribution systems. FLA were isolated using the amoebal enrichment technique and identified using PCR and 18S rDNA sequencing. Amoebae potentially containing intra-amoebal bacteria were lysed and cultured on blood agar plates. Bacterial isolates were characterized using the VITEK®2 compact System. Free-living amoebae were isolated from 77 (43.3 %) of the samples. Using microscopy, PCR and 18S rRNA sequencing, Acanthamoeba spp. (T3 and T20 genotypes), Vermamoeba vermiformis and Naegleria gruberi specie were identified. The Acanthamoeba T3 and T20 genotypes have been implicated in eye and central nervous system infections. The most commonly detected bacterial species were Serratia marcescens, Stenotrophomonas maltophilia, Delftia acidovorans, Sphingomonas paucimobilis and Comamonas testosteroni. These nosocomial pathogenic bacteria are associated with systematic blood, respiratory tract, the urinary tract, surgical wounds and soft tissues infections. The detection of FLA and their associated opportunistic bacteria in the hospital water systems point out to a potential health risk to immune-compromised individuals. SN - 1432-1955 UR - https://www.unboundmedicine.com/medline/citation/27730363/Coexistence_of_free_living_amoebae_and_bacteria_in_selected_South_African_hospital_water_distribution_systems_ L2 - https://dx.doi.org/10.1007/s00436-016-5271-3 DB - PRIME DP - Unbound Medicine ER -