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β-asarone improves learning and memory and reduces Acetyl Cholinesterase and Beta-amyloid 42 levels in APP/PS1 transgenic mice by regulating Beclin-1-dependent autophagy.
Brain Res. 2016 12 01; 1652:188-194.BR

Abstract

Alzheimer's disease (AD) is the most common neurodegenerative disorder in the elderly, and studies have suggested that β-asarone has pharmacological effects on beta-amyloid (Aβ) injected in the rat hippocampus. However, the effect of β-asarone on autophagy in the APP/PS1 transgenic mouse is unreported. APP/PS1 transgenic mice were randomly divided into six groups (n=10/group): an untreated group, an Aricept-treated group, a 3-MA-treated group, a rapamycin-treated group, an LY294002-treated group, a β-asarone-treated group. The control group consisted of wild-type C57BL/6 mice. All treatments were administered to the mice for 30 days. Spatial learning and memory were assessed by water maze, passive avoidance, and step-down tests. AChE and Aβ42 levels in the hippocampus were determined by ELISA. p-Akt, p-mTOR, and LC3B expression were detected by flow cytometry. The expression of p-Akt, p-mTOR, Beclin-1, and p62 proteins was assessed by western blot. Changes in autophagy were viewed using a transmission electron microscope. APP and Beclin-1 mRNA levels were measured by Real-Time PCR. The learning and memory of APP/PS1 transgenic mice were improved significantly after β-asarone treatment compared with the untreated group. In addition, β-asarone treatment reduced AChE and Aβ42 levels, increased p-mTOR and p62 expression, decreased p-Akt, Beclin-1, and LC3B expression, decreased the number of autophagosomes and reduced APP mRNA and Beclin-1 mRNA levels compared with the untreated group. That is, β-asarone treatment can improve the learning and memory abilities of APP/PS1 transgenic mouse by inhibiting Beclin-1-dependent autophagy via the PI3K/Akt/mTOR pathway.

Authors+Show Affiliations

Guangdong Provincial Hospital of Chinese Medicine, Guangdong Provincial Academy of Chinese Medical Sciences, and The Second Clinical College, Guangzhou University of Chinese Medicine, Guangzhou 510120, PR China; Guangzhou University of Chinese Medicine, Guangzhou 510006, PR China.Hainan Medical University, Haikou 571199, PR China; Lingnan Normal University, Zhanjiang 524048, PR China.Guangzhou University of Chinese Medicine, Guangzhou 510006, PR China.Guangzhou University of Chinese Medicine, Guangzhou 510006, PR China.Guangzhou University of Chinese Medicine, Guangzhou 510006, PR China.Guangzhou University of Chinese Medicine, Guangzhou 510006, PR China.Guangzhou University of Chinese Medicine, Guangzhou 510006, PR China; The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 510405, PR China. Electronic address: fangyq2@163.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27737765

Citation

Deng, Minzhen, et al. "Β-asarone Improves Learning and Memory and Reduces Acetyl Cholinesterase and Beta-amyloid 42 Levels in APP/PS1 Transgenic Mice By Regulating Beclin-1-dependent Autophagy." Brain Research, vol. 1652, 2016, pp. 188-194.
Deng M, Huang L, Ning B, et al. Β-asarone improves learning and memory and reduces Acetyl Cholinesterase and Beta-amyloid 42 levels in APP/PS1 transgenic mice by regulating Beclin-1-dependent autophagy. Brain Res. 2016;1652:188-194.
Deng, M., Huang, L., Ning, B., Wang, N., Zhang, Q., Zhu, C., & Fang, Y. (2016). Β-asarone improves learning and memory and reduces Acetyl Cholinesterase and Beta-amyloid 42 levels in APP/PS1 transgenic mice by regulating Beclin-1-dependent autophagy. Brain Research, 1652, 188-194. https://doi.org/10.1016/j.brainres.2016.10.008
Deng M, et al. Β-asarone Improves Learning and Memory and Reduces Acetyl Cholinesterase and Beta-amyloid 42 Levels in APP/PS1 Transgenic Mice By Regulating Beclin-1-dependent Autophagy. Brain Res. 2016 12 1;1652:188-194. PubMed PMID: 27737765.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - β-asarone improves learning and memory and reduces Acetyl Cholinesterase and Beta-amyloid 42 levels in APP/PS1 transgenic mice by regulating Beclin-1-dependent autophagy. AU - Deng,Minzhen, AU - Huang,Liping, AU - Ning,Baile, AU - Wang,Nanbu, AU - Zhang,Qinxin, AU - Zhu,Caixia, AU - Fang,Yongqi, Y1 - 2016/10/11/ PY - 2016/01/20/received PY - 2016/10/07/revised PY - 2016/10/09/accepted PY - 2016/10/16/pubmed PY - 2017/7/29/medline PY - 2016/10/15/entrez KW - APP/PS1 transgenic mice KW - Alzheimer’s disease KW - Autophagy KW - PI3K/Akt/mTOR pathway KW - β-asarone SP - 188 EP - 194 JF - Brain research JO - Brain Res. VL - 1652 N2 - Alzheimer's disease (AD) is the most common neurodegenerative disorder in the elderly, and studies have suggested that β-asarone has pharmacological effects on beta-amyloid (Aβ) injected in the rat hippocampus. However, the effect of β-asarone on autophagy in the APP/PS1 transgenic mouse is unreported. APP/PS1 transgenic mice were randomly divided into six groups (n=10/group): an untreated group, an Aricept-treated group, a 3-MA-treated group, a rapamycin-treated group, an LY294002-treated group, a β-asarone-treated group. The control group consisted of wild-type C57BL/6 mice. All treatments were administered to the mice for 30 days. Spatial learning and memory were assessed by water maze, passive avoidance, and step-down tests. AChE and Aβ42 levels in the hippocampus were determined by ELISA. p-Akt, p-mTOR, and LC3B expression were detected by flow cytometry. The expression of p-Akt, p-mTOR, Beclin-1, and p62 proteins was assessed by western blot. Changes in autophagy were viewed using a transmission electron microscope. APP and Beclin-1 mRNA levels were measured by Real-Time PCR. The learning and memory of APP/PS1 transgenic mice were improved significantly after β-asarone treatment compared with the untreated group. In addition, β-asarone treatment reduced AChE and Aβ42 levels, increased p-mTOR and p62 expression, decreased p-Akt, Beclin-1, and LC3B expression, decreased the number of autophagosomes and reduced APP mRNA and Beclin-1 mRNA levels compared with the untreated group. That is, β-asarone treatment can improve the learning and memory abilities of APP/PS1 transgenic mouse by inhibiting Beclin-1-dependent autophagy via the PI3K/Akt/mTOR pathway. SN - 1872-6240 UR - https://www.unboundmedicine.com/medline/citation/27737765/β_asarone_improves_learning_and_memory_and_reduces_Acetyl_Cholinesterase_and_Beta_amyloid_42_levels_in_APP/PS1_transgenic_mice_by_regulating_Beclin_1_dependent_autophagy_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-8993(16)30700-4 DB - PRIME DP - Unbound Medicine ER -