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Development of eosinophilic inflammation is independent of B-T cell interaction in a chronic house dust mite-driven asthma model.
Clin Exp Allergy 2017; 47(4):551-564CE

Abstract

BACKGROUND

Chronic exposure to environmental triggers, such as house dust mite (HDM), drives T helper 2 (Th2) cell-mediated asthma. Recent evidence has shown that B-T cell interaction, and in particular germinal centre reactions and follicular T helper (Tfh) cells are required for the development of eosinophilic airway inflammation in HDM-driven models containing a sensitization and challenge phase. Whether B-T cell interactions are essential for pulmonary eosinophilic inflammation following chronic allergen provocation remains unknown.

AIMS

In this study, we investigated the importance of B-T cell interaction in the development of eosinophilic airway inflammation and pulmonary remodelling in a chronic HDM-driven asthma model.

METHODS

We exposed C57BL/6, Cd40l-/- , and Mb1-/- mice to HDM three times a week for five consecutive weeks.

RESULTS

Chronic HDM exposure induced a pronounced eosinophilic allergic airway inflammation in broncho-alveolar lavage fluid (BALf) and lung tissue, associated with the formation of immunologically active inducible bronchus-associated lymphoid tissue (iBALT) in the lungs. The absence of B cells or lack of CD40L signalling did not hamper eosinophilic inflammation in the airways, although the number of Tfh and Th2 cells was substantially reduced in the lungs. Importantly, type 2 innate lymphoid cell (ILC2) numbers in BALf and lung were not affected by the absence of B cells or B-T cell interaction. Furthermore, eosinophilic airway inflammation is not sufficient to induce pulmonary remodelling and airway hyperresponsiveness.

CONCLUSION AND CLINICAL RELEVANCE

From these findings, we conclude that B-T cell interaction is required for robust Tfh and Th2 cell induction, but not essential for eosinophilic airway inflammation during a chronic HDM-driven asthma model.

Authors+Show Affiliations

Department of Pulmonary Medicine, Erasmus MC, Rotterdam, The Netherlands.Department of Pulmonary Medicine, Erasmus MC, Rotterdam, The Netherlands.Department of Pulmonary Medicine, Erasmus MC, Rotterdam, The Netherlands.Department of Pulmonary Medicine, Erasmus MC, Rotterdam, The Netherlands.Department of Pulmonary Medicine, Erasmus MC, Rotterdam, The Netherlands.Department of Pulmonary Medicine, Erasmus MC, Rotterdam, The Netherlands.Department of Pulmonary Medicine, Erasmus MC, Rotterdam, The Netherlands.Department of Pulmonary Medicine, Erasmus MC, Rotterdam, The Netherlands.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27743410

Citation

Vroman, H, et al. "Development of Eosinophilic Inflammation Is Independent of B-T Cell Interaction in a Chronic House Dust Mite-driven Asthma Model." Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology, vol. 47, no. 4, 2017, pp. 551-564.
Vroman H, Bergen IM, Li BW, et al. Development of eosinophilic inflammation is independent of B-T cell interaction in a chronic house dust mite-driven asthma model. Clin Exp Allergy. 2017;47(4):551-564.
Vroman, H., Bergen, I. M., Li, B. W., van Hulst, J. A., Lukkes, M., van Uden, D., ... Kool, M. (2017). Development of eosinophilic inflammation is independent of B-T cell interaction in a chronic house dust mite-driven asthma model. Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology, 47(4), pp. 551-564. doi:10.1111/cea.12834.
Vroman H, et al. Development of Eosinophilic Inflammation Is Independent of B-T Cell Interaction in a Chronic House Dust Mite-driven Asthma Model. Clin Exp Allergy. 2017;47(4):551-564. PubMed PMID: 27743410.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development of eosinophilic inflammation is independent of B-T cell interaction in a chronic house dust mite-driven asthma model. AU - Vroman,H, AU - Bergen,I M, AU - Li,B W S, AU - van Hulst,J A C, AU - Lukkes,M, AU - van Uden,D, AU - Hendriks,R W, AU - Kool,M, Y1 - 2016/11/22/ PY - 2016/05/31/received PY - 2016/08/30/revised PY - 2016/09/07/accepted PY - 2016/10/16/pubmed PY - 2017/12/23/medline PY - 2016/10/16/entrez KW - B cells KW - Tfh cells KW - Th2 cells KW - asthma KW - house dust mite SP - 551 EP - 564 JF - Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology JO - Clin. Exp. Allergy VL - 47 IS - 4 N2 - BACKGROUND: Chronic exposure to environmental triggers, such as house dust mite (HDM), drives T helper 2 (Th2) cell-mediated asthma. Recent evidence has shown that B-T cell interaction, and in particular germinal centre reactions and follicular T helper (Tfh) cells are required for the development of eosinophilic airway inflammation in HDM-driven models containing a sensitization and challenge phase. Whether B-T cell interactions are essential for pulmonary eosinophilic inflammation following chronic allergen provocation remains unknown. AIMS: In this study, we investigated the importance of B-T cell interaction in the development of eosinophilic airway inflammation and pulmonary remodelling in a chronic HDM-driven asthma model. METHODS: We exposed C57BL/6, Cd40l-/- , and Mb1-/- mice to HDM three times a week for five consecutive weeks. RESULTS: Chronic HDM exposure induced a pronounced eosinophilic allergic airway inflammation in broncho-alveolar lavage fluid (BALf) and lung tissue, associated with the formation of immunologically active inducible bronchus-associated lymphoid tissue (iBALT) in the lungs. The absence of B cells or lack of CD40L signalling did not hamper eosinophilic inflammation in the airways, although the number of Tfh and Th2 cells was substantially reduced in the lungs. Importantly, type 2 innate lymphoid cell (ILC2) numbers in BALf and lung were not affected by the absence of B cells or B-T cell interaction. Furthermore, eosinophilic airway inflammation is not sufficient to induce pulmonary remodelling and airway hyperresponsiveness. CONCLUSION AND CLINICAL RELEVANCE: From these findings, we conclude that B-T cell interaction is required for robust Tfh and Th2 cell induction, but not essential for eosinophilic airway inflammation during a chronic HDM-driven asthma model. SN - 1365-2222 UR - https://www.unboundmedicine.com/medline/citation/27743410/Development_of_eosinophilic_inflammation_is_independent_of_B_T_cell_interaction_in_a_chronic_house_dust_mite_driven_asthma_model_ L2 - https://doi.org/10.1111/cea.12834 DB - PRIME DP - Unbound Medicine ER -