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Transcriptomic and Proteomic Analysis of Oenococcus oeni Adaptation to Wine Stress Conditions.
Front Microbiol 2016; 7:1554FM

Abstract

Oenococcus oeni, the main lactic acid bacteria responsible for malolactic fermentation in wine, has to adapt to stressful conditions, such as low pH and high ethanol content. In this study, the changes in the transcriptome and the proteome of O. oeni PSU-1 during the adaptation period before MLF start have been studied. DNA microarrays were used for the transcriptomic analysis and two complementary proteomic techniques, 2-D DIGE and iTRAQ labeling were used to analyze the proteomic response. One of the most influenced functions in PSU-1 due to inoculation into wine-like medium (WLM) was translation, showing the over-expression of certain ribosomal genes and the corresponding proteins. Amino acid metabolism and transport was also altered and several peptidases were up regulated both at gene and protein level. Certain proteins involved in glutamine and glutamate metabolism showed an increased abundance revealing the key role of nitrogen uptake under stressful conditions. A strong transcriptional inhibition of carbohydrate metabolism related genes was observed. On the other hand, the transcriptional up-regulation of malate transport and citrate consumption was indicative of the use of L-malate and citrate associated to stress response and as an alternative energy source to sugar metabolism. Regarding the stress mechanisms, our results support the relevance of the thioredoxin and glutathione systems in the adaptation of O. oeni to wine related stress. Genes and proteins related to cell wall showed also significant changes indicating the relevance of the cell envelop as protective barrier to environmental stress. The differences found between transcriptomic and proteomic data suggested the relevance of post-transcriptional mechanisms and the complexity of the stress response in O. oeni adaptation. Further research should deepen into the metabolisms mostly altered due to wine conditions to elucidate the role of each mechanism in the O. oeni ability to develop MLF.

Authors+Show Affiliations

Departament de Bioquímica i Biotecnologia, Facultat d'Enologia, Universitat Rovira i Virgili Tarragona, Spain.Departament de Bioquímica i Biotecnologia, Facultat d'Enologia, Universitat Rovira i Virgili Tarragona, Spain.Departament de Bioquímica i Biotecnologia, Facultat d'Enologia, Universitat Rovira i Virgili Tarragona, Spain.Departament de Bioquímica i Biotecnologia, Facultat d'Enologia, Universitat Rovira i Virgili Tarragona, Spain.Departament de Bioquímica i Biotecnologia, Facultat d'Enologia, Universitat Rovira i Virgili Tarragona, Spain.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27746771

Citation

Margalef-Català, Mar, et al. "Transcriptomic and Proteomic Analysis of Oenococcus Oeni Adaptation to Wine Stress Conditions." Frontiers in Microbiology, vol. 7, 2016, p. 1554.
Margalef-Català M, Araque I, Bordons A, et al. Transcriptomic and Proteomic Analysis of Oenococcus oeni Adaptation to Wine Stress Conditions. Front Microbiol. 2016;7:1554.
Margalef-Català, M., Araque, I., Bordons, A., Reguant, C., & Bautista-Gallego, J. (2016). Transcriptomic and Proteomic Analysis of Oenococcus oeni Adaptation to Wine Stress Conditions. Frontiers in Microbiology, 7, p. 1554.
Margalef-Català M, et al. Transcriptomic and Proteomic Analysis of Oenococcus Oeni Adaptation to Wine Stress Conditions. Front Microbiol. 2016;7:1554. PubMed PMID: 27746771.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Transcriptomic and Proteomic Analysis of Oenococcus oeni Adaptation to Wine Stress Conditions. AU - Margalef-Català,Mar, AU - Araque,Isabel, AU - Bordons,Albert, AU - Reguant,Cristina, AU - Bautista-Gallego,Joaquín, Y1 - 2016/09/30/ PY - 2016/08/08/received PY - 2016/09/16/accepted PY - 2016/10/18/entrez PY - 2016/10/18/pubmed PY - 2016/10/18/medline KW - Oenococcus oeni KW - malolactic fermentation KW - proteomic KW - stress KW - transcriptomic KW - wine SP - 1554 EP - 1554 JF - Frontiers in microbiology JO - Front Microbiol VL - 7 N2 - Oenococcus oeni, the main lactic acid bacteria responsible for malolactic fermentation in wine, has to adapt to stressful conditions, such as low pH and high ethanol content. In this study, the changes in the transcriptome and the proteome of O. oeni PSU-1 during the adaptation period before MLF start have been studied. DNA microarrays were used for the transcriptomic analysis and two complementary proteomic techniques, 2-D DIGE and iTRAQ labeling were used to analyze the proteomic response. One of the most influenced functions in PSU-1 due to inoculation into wine-like medium (WLM) was translation, showing the over-expression of certain ribosomal genes and the corresponding proteins. Amino acid metabolism and transport was also altered and several peptidases were up regulated both at gene and protein level. Certain proteins involved in glutamine and glutamate metabolism showed an increased abundance revealing the key role of nitrogen uptake under stressful conditions. A strong transcriptional inhibition of carbohydrate metabolism related genes was observed. On the other hand, the transcriptional up-regulation of malate transport and citrate consumption was indicative of the use of L-malate and citrate associated to stress response and as an alternative energy source to sugar metabolism. Regarding the stress mechanisms, our results support the relevance of the thioredoxin and glutathione systems in the adaptation of O. oeni to wine related stress. Genes and proteins related to cell wall showed also significant changes indicating the relevance of the cell envelop as protective barrier to environmental stress. The differences found between transcriptomic and proteomic data suggested the relevance of post-transcriptional mechanisms and the complexity of the stress response in O. oeni adaptation. Further research should deepen into the metabolisms mostly altered due to wine conditions to elucidate the role of each mechanism in the O. oeni ability to develop MLF. SN - 1664-302X UR - https://www.unboundmedicine.com/medline/citation/27746771/Transcriptomic_and_Proteomic_Analysis_of_Oenococcus_oeni_Adaptation_to_Wine_Stress_Conditions_ L2 - https://dx.doi.org/10.3389/fmicb.2016.01554 DB - PRIME DP - Unbound Medicine ER -