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Evaluation of dermal wound healing and in vitro antioxidant efficiency of Bacillus subtilis SPB1 biosurfactant.
Biomed Pharmacother. 2016 Dec; 84:878-891.BP

Abstract

BACKGROUND

Lipopeptide microbial surfactants are endowed with unique surface properties as well as antimicrobial, anti-wrinkle, moisturizing and free radical scavenging activities. They were introduced safely in dermatological products, as long as they present low cytotoxicity against human cells. The present study was undertaken to evaluate the in vitro antioxidant activities and the wound healing potential of Bacillus subtilis SPB1 lipopeptide biosurfactant on excision wounds induced in experimental rats.

RESULTS

The scavenging effect of Bacillus subtilis SPB1 biosurfactant on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical at 1mg/mL was 70.4% (IC50=0.55mg/mL). The biosurfactant produced by Bacillus subtilis SPB1 also showed good reducing power and significant effects in terms of the β-carotene test (IC50=2.26mg/mL) when compared to BHA as a reference standard. Moreover, an interesting ferrous ion chelating activity (80.32%) was found for SPB1 biosurfactant at 1mg/mL. Furthermore, the topical application of Bacillus subtilis SPB1 biosurfactant based gel on the wound site in a rat model every two days, increased significantly the percentage of wound closure over a period of 13days, when compared to the untreated and CICAFLORA™-treated groups. Wound healing effect of SPB1 biosurfactant based gel was confirmed by histological study. Biopsies treated with SPB1 lipopeptides showed wholly re-epithelialized wound with a perfect epidermal regeneration.

CONCLUSIONS

The present study provides justification for the use of Bacillus subtilis SPB1 lipopeptide biosurfactant based gel for the treatment of normal and complicated wounds as well as skin diseases.

Authors+Show Affiliations

Enzymes and Bioconversion Research Unit, Department of biological engineering, National School of Engineers of Sfax (ENIS), BP W, 3038 Sfax, Tunisia; Higher Institute of Biotechnology of Sfax (ISBS), BP 261, 3000 Sfax, Tunisia.Laboratory of Pharmacology, Faculty of Medicine of Sfax, 3028 Sfax, Tunisia.Laboratory of Pharmacology, Faculty of Medicine of Sfax, 3028 Sfax, Tunisia.Laboratory of Histology Embryology and Reproductive Biology, Faculty of Medicine of Sfax, 3028 Sfax, Tunisia.Enzymes and Bioconversion Research Unit, Department of biological engineering, National School of Engineers of Sfax (ENIS), BP W, 3038 Sfax, Tunisia.Enzymes and Bioconversion Research Unit, Department of biological engineering, National School of Engineers of Sfax (ENIS), BP W, 3038 Sfax, Tunisia; Higher Institute of Biotechnology of Sfax (ISBS), BP 261, 3000 Sfax, Tunisia. Electronic address: dhouhag@yahoo.fr.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27750203

Citation

Zouari, Raida, et al. "Evaluation of Dermal Wound Healing and in Vitro Antioxidant Efficiency of Bacillus Subtilis SPB1 Biosurfactant." Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie, vol. 84, 2016, pp. 878-891.
Zouari R, Moalla-Rekik D, Sahnoun Z, et al. Evaluation of dermal wound healing and in vitro antioxidant efficiency of Bacillus subtilis SPB1 biosurfactant. Biomed Pharmacother. 2016;84:878-891.
Zouari, R., Moalla-Rekik, D., Sahnoun, Z., Rebai, T., Ellouze-Chaabouni, S., & Ghribi-Aydi, D. (2016). Evaluation of dermal wound healing and in vitro antioxidant efficiency of Bacillus subtilis SPB1 biosurfactant. Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie, 84, 878-891. https://doi.org/10.1016/j.biopha.2016.09.084
Zouari R, et al. Evaluation of Dermal Wound Healing and in Vitro Antioxidant Efficiency of Bacillus Subtilis SPB1 Biosurfactant. Biomed Pharmacother. 2016;84:878-891. PubMed PMID: 27750203.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of dermal wound healing and in vitro antioxidant efficiency of Bacillus subtilis SPB1 biosurfactant. AU - Zouari,Raida, AU - Moalla-Rekik,Dorsaf, AU - Sahnoun,Zouheir, AU - Rebai,Tarek, AU - Ellouze-Chaabouni,Semia, AU - Ghribi-Aydi,Dhouha, Y1 - 2016/10/14/ PY - 2016/07/01/received PY - 2016/08/19/revised PY - 2016/10/18/pubmed PY - 2017/2/14/medline PY - 2016/10/18/entrez KW - Antioxidant activity KW - Bacillus subtilis KW - Biosurfactant KW - Lipopeptides KW - Wound-healing efficiency SP - 878 EP - 891 JF - Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie JO - Biomed Pharmacother VL - 84 N2 - BACKGROUND: Lipopeptide microbial surfactants are endowed with unique surface properties as well as antimicrobial, anti-wrinkle, moisturizing and free radical scavenging activities. They were introduced safely in dermatological products, as long as they present low cytotoxicity against human cells. The present study was undertaken to evaluate the in vitro antioxidant activities and the wound healing potential of Bacillus subtilis SPB1 lipopeptide biosurfactant on excision wounds induced in experimental rats. RESULTS: The scavenging effect of Bacillus subtilis SPB1 biosurfactant on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical at 1mg/mL was 70.4% (IC50=0.55mg/mL). The biosurfactant produced by Bacillus subtilis SPB1 also showed good reducing power and significant effects in terms of the β-carotene test (IC50=2.26mg/mL) when compared to BHA as a reference standard. Moreover, an interesting ferrous ion chelating activity (80.32%) was found for SPB1 biosurfactant at 1mg/mL. Furthermore, the topical application of Bacillus subtilis SPB1 biosurfactant based gel on the wound site in a rat model every two days, increased significantly the percentage of wound closure over a period of 13days, when compared to the untreated and CICAFLORA™-treated groups. Wound healing effect of SPB1 biosurfactant based gel was confirmed by histological study. Biopsies treated with SPB1 lipopeptides showed wholly re-epithelialized wound with a perfect epidermal regeneration. CONCLUSIONS: The present study provides justification for the use of Bacillus subtilis SPB1 lipopeptide biosurfactant based gel for the treatment of normal and complicated wounds as well as skin diseases. SN - 1950-6007 UR - https://www.unboundmedicine.com/medline/citation/27750203/Evaluation_of_dermal_wound_healing_and_in_vitro_antioxidant_efficiency_of_Bacillus_subtilis_SPB1_biosurfactant_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0753-3322(16)30968-4 DB - PRIME DP - Unbound Medicine ER -