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Optimization of ultrasonic-assisted extraction of antioxidant polysaccharides from the stem of Trapa quadrispinosa using response surface methodology.
Int J Biol Macromol. 2017 Jan; 94(Pt A):335-344.IJ

Abstract

Response surface methodology (RSM) was used to optimize experimental conditions for ultrasonic-assisted extraction (UAE) of antioxidant crude polysaccharides (CPS) from Trapa quadrispinosa stems. Antioxidant capacity of polysaccharides was determined by Ferric-Reducing Antioxidant Capacity (FRAC) assay with the method of 1, 10-phenanthroline. The maximum yield of polysaccharides (2.78±0.16%) was obtained under optimal extraction conditions of extraction time, 41min; ratio of water to material, 31.5mL/g; and extraction temperature, 58°C. The maximum antioxidant capacity (19.02±0.24μmol Fe2+/g) was obtained under the optimal extraction conditions of extraction time, 38min; ratio of water to material, 32mL/g; and extraction temperature, 56°C. These two values were agreed well with predicted yield (2.75%) and antioxidant capacity (18.77μmol Fe2+/g). Antioxidant activities of CPS were investigated by various assays. The results showed that CPSUAE obtained by UAE showed higher 1,1-Diphenyl-2-picrylhydrazxyl (DPPH), 2,2-azinobis(3-ethylbenzthiazoline)-6-sulfonic acid (ABTS) radical scavenging activities and stronger reducing power, total antioxidant capacity compared with CPSHWE obtained by hot water extraction (HWE).The results indicated that UAE is an advisable method for extraction of antioxidant polysaccharides from the stem of T. quadrispinosa and polysaccharides could be explored as potential antioxidant to use in medicine or functional food.

Authors+Show Affiliations

School of Pharmacy, Jiangsu University, Zhenjiang, 212013, PR China.Affiliated Hospital of Jiangsu University, Zhenjiang, 212001, PR China; School of Food and Biological Engineering, Jiangsu University, Zhenjiang, 212013, PR China.School of Pharmacy, Jiangsu University, Zhenjiang, 212013, PR China. Electronic address: xxq781026@ujs.edu.cn.School of Pharmacy, Jiangsu University, Zhenjiang, 212013, PR China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27751809

Citation

Raza, Aun, et al. "Optimization of Ultrasonic-assisted Extraction of Antioxidant Polysaccharides From the Stem of Trapa Quadrispinosa Using Response Surface Methodology." International Journal of Biological Macromolecules, vol. 94, no. Pt A, 2017, pp. 335-344.
Raza A, Li F, Xu X, et al. Optimization of ultrasonic-assisted extraction of antioxidant polysaccharides from the stem of Trapa quadrispinosa using response surface methodology. Int J Biol Macromol. 2017;94(Pt A):335-344.
Raza, A., Li, F., Xu, X., & Tang, J. (2017). Optimization of ultrasonic-assisted extraction of antioxidant polysaccharides from the stem of Trapa quadrispinosa using response surface methodology. International Journal of Biological Macromolecules, 94(Pt A), 335-344. https://doi.org/10.1016/j.ijbiomac.2016.10.033
Raza A, et al. Optimization of Ultrasonic-assisted Extraction of Antioxidant Polysaccharides From the Stem of Trapa Quadrispinosa Using Response Surface Methodology. Int J Biol Macromol. 2017;94(Pt A):335-344. PubMed PMID: 27751809.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Optimization of ultrasonic-assisted extraction of antioxidant polysaccharides from the stem of Trapa quadrispinosa using response surface methodology. AU - Raza,Aun, AU - Li,Feng, AU - Xu,Xiuquan, AU - Tang,Jian, Y1 - 2016/10/14/ PY - 2016/03/30/received PY - 2016/10/07/revised PY - 2016/10/13/accepted PY - 2016/10/30/pubmed PY - 2017/4/22/medline PY - 2016/10/30/entrez KW - Antioxidant polysaccharides KW - Response surface methodology KW - Trapa quadrispinosa Roxb. KW - Ultrasonic-assisted extraction SP - 335 EP - 344 JF - International journal of biological macromolecules JO - Int. J. Biol. Macromol. VL - 94 IS - Pt A N2 - Response surface methodology (RSM) was used to optimize experimental conditions for ultrasonic-assisted extraction (UAE) of antioxidant crude polysaccharides (CPS) from Trapa quadrispinosa stems. Antioxidant capacity of polysaccharides was determined by Ferric-Reducing Antioxidant Capacity (FRAC) assay with the method of 1, 10-phenanthroline. The maximum yield of polysaccharides (2.78±0.16%) was obtained under optimal extraction conditions of extraction time, 41min; ratio of water to material, 31.5mL/g; and extraction temperature, 58°C. The maximum antioxidant capacity (19.02±0.24μmol Fe2+/g) was obtained under the optimal extraction conditions of extraction time, 38min; ratio of water to material, 32mL/g; and extraction temperature, 56°C. These two values were agreed well with predicted yield (2.75%) and antioxidant capacity (18.77μmol Fe2+/g). Antioxidant activities of CPS were investigated by various assays. The results showed that CPSUAE obtained by UAE showed higher 1,1-Diphenyl-2-picrylhydrazxyl (DPPH), 2,2-azinobis(3-ethylbenzthiazoline)-6-sulfonic acid (ABTS) radical scavenging activities and stronger reducing power, total antioxidant capacity compared with CPSHWE obtained by hot water extraction (HWE).The results indicated that UAE is an advisable method for extraction of antioxidant polysaccharides from the stem of T. quadrispinosa and polysaccharides could be explored as potential antioxidant to use in medicine or functional food. SN - 1879-0003 UR - https://www.unboundmedicine.com/medline/citation/27751809/Optimization_of_ultrasonic_assisted_extraction_of_antioxidant_polysaccharides_from_the_stem_of_Trapa_quadrispinosa_using_response_surface_methodology_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0141-8130(16)31994-8 DB - PRIME DP - Unbound Medicine ER -