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Senescence-specific change in ROS scavenging enzyme activities and regulation of various SOD isozymes to ROS levels in psf mutant rice leaves.
Plant Physiol Biochem. 2016 Dec; 109:248-261.PP

Abstract

To clarify the interaction between different antioxidant enzymes for monitoring oxidative stress and ROS burst in rice senescent leaves, we investigated the genotype-dependent alteration in temporal patterns of the O2•- production rate, H2O2 content, and ROS-scavenging enzyme activities during leaf senescence in two rice genotypes, namely, the premature senescence of flag leaf (psf) mutant and its wild type. Results showed that the psf mutant differed obviously from its wild type in leaf O2•- generation rate and H2O2 content accumulation, and the decreased activities of SOD, CAT, and APX in the psf leaves were strongly responsible for the increased ROS level and the accelerated leaf senescence. By contrast, the increase in POD activity was positively correlated with the senescence-related enhancement in O2•- generation in rice leaves. Among various SOD isoforms, Mn-SOD responded sensitively to the increasing O2•- generation rate, whereas Cu/Zn-SOD remained stable with the progression of leaf senescence. These findings suggest that the senescence-related decline in total SOD activity was mostly attributable to the downregulation of both the translation and transcription of Mn-SOD isoform. This occurrence finally resulted in the collapse of SOD defense system and accelerated leaf senescence for the psf mutant. Furthermore, we presented the possible contribution of several Cu/Zn-SOD expression patterns to the senescence-related O2•- detoxification in different cell compartments.

Authors+Show Affiliations

Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 310058, China; Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing, China.Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 310058, China.Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 310058, China; Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing, China.Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 310058, China.Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 310058, China; Institute of Crop Science, Fujian Agricultural and Forest University, Fuzhou, 310058, China.Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 310058, China; Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing, China.Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 310058, China; Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing, China. Electronic address: chengfm@zju.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27756006

Citation

Wang, Fubiao, et al. "Senescence-specific Change in ROS Scavenging Enzyme Activities and Regulation of Various SOD Isozymes to ROS Levels in Psf Mutant Rice Leaves." Plant Physiology and Biochemistry : PPB, vol. 109, 2016, pp. 248-261.
Wang F, Liu J, Zhou L, et al. Senescence-specific change in ROS scavenging enzyme activities and regulation of various SOD isozymes to ROS levels in psf mutant rice leaves. Plant Physiol Biochem. 2016;109:248-261.
Wang, F., Liu, J., Zhou, L., Pan, G., Li, Z., Zaidi, S. H., & Cheng, F. (2016). Senescence-specific change in ROS scavenging enzyme activities and regulation of various SOD isozymes to ROS levels in psf mutant rice leaves. Plant Physiology and Biochemistry : PPB, 109, 248-261. https://doi.org/10.1016/j.plaphy.2016.10.005
Wang F, et al. Senescence-specific Change in ROS Scavenging Enzyme Activities and Regulation of Various SOD Isozymes to ROS Levels in Psf Mutant Rice Leaves. Plant Physiol Biochem. 2016;109:248-261. PubMed PMID: 27756006.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Senescence-specific change in ROS scavenging enzyme activities and regulation of various SOD isozymes to ROS levels in psf mutant rice leaves. AU - Wang,Fubiao, AU - Liu,Jianchao, AU - Zhou,Lujian, AU - Pan,Gang, AU - Li,Zhaowei, AU - Zaidi,Syed-Hassan-Raza, AU - Cheng,Fangmin, Y1 - 2016/10/11/ PY - 2016/07/12/received PY - 2016/10/08/revised PY - 2016/10/08/accepted PY - 2016/10/19/pubmed PY - 2017/4/11/medline PY - 2016/10/19/entrez KW - Antioxidant enzymes KW - Leaf senescence KW - Methyl viologen (MV) KW - Reactive oxygen species (ROS) KW - SOD isoforms SP - 248 EP - 261 JF - Plant physiology and biochemistry : PPB JO - Plant Physiol Biochem VL - 109 N2 - To clarify the interaction between different antioxidant enzymes for monitoring oxidative stress and ROS burst in rice senescent leaves, we investigated the genotype-dependent alteration in temporal patterns of the O2•- production rate, H2O2 content, and ROS-scavenging enzyme activities during leaf senescence in two rice genotypes, namely, the premature senescence of flag leaf (psf) mutant and its wild type. Results showed that the psf mutant differed obviously from its wild type in leaf O2•- generation rate and H2O2 content accumulation, and the decreased activities of SOD, CAT, and APX in the psf leaves were strongly responsible for the increased ROS level and the accelerated leaf senescence. By contrast, the increase in POD activity was positively correlated with the senescence-related enhancement in O2•- generation in rice leaves. Among various SOD isoforms, Mn-SOD responded sensitively to the increasing O2•- generation rate, whereas Cu/Zn-SOD remained stable with the progression of leaf senescence. These findings suggest that the senescence-related decline in total SOD activity was mostly attributable to the downregulation of both the translation and transcription of Mn-SOD isoform. This occurrence finally resulted in the collapse of SOD defense system and accelerated leaf senescence for the psf mutant. Furthermore, we presented the possible contribution of several Cu/Zn-SOD expression patterns to the senescence-related O2•- detoxification in different cell compartments. SN - 1873-2690 UR - https://www.unboundmedicine.com/medline/citation/27756006/Senescence_specific_change_in_ROS_scavenging_enzyme_activities_and_regulation_of_various_SOD_isozymes_to_ROS_levels_in_psf_mutant_rice_leaves_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0981-9428(16)30389-8 DB - PRIME DP - Unbound Medicine ER -