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Porcine circovirus 2 proliferation can be enhanced by stably expressing porcine IL-2 gene in PK-15 cell.
Virus Res. 2017 01 02; 227:143-149.VR

Abstract

Porcine circovirus 2 (PCV2) is the causative agent of porcine circovirus diseases and porcine circovirus-associated diseases (PCVD/PCVAD), which are widely present in every major swine farm. However, lower propagation rate of PCV2 in vitro seriously hindered the production of PCV2 vaccine. Previously, we found that interleukin-2 (IL-2) can increase PCV2 yield in vitro. In the present study, porcine IL-2 gene was amplified and stably transfected into PK-15 cells. The results demonstrated that PCV2 proliferation can be significantly enhanced in cells stably expressing porcine IL-2 gene, suggesting that porcine IL-2 contributes to proliferation of PCV2. These results indicated that cells overexpressing porcine IL-2 gene can be used as a promising cell line for vaccine development of PCV2.

Authors+Show Affiliations

Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun, Jilin 130062, PR China.Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun, Jilin 130062, PR China.Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun, Jilin 130062, PR China.Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun, Jilin 130062, PR China.Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun, Jilin 130062, PR China.Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun, Jilin 130062, PR China.Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun, Jilin 130062, PR China.Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun, Jilin 130062, PR China. Electronic address: renlz@jlu.edu.cn.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27760373

Citation

Ma, Teng, et al. "Porcine Circovirus 2 Proliferation Can Be Enhanced By Stably Expressing Porcine IL-2 Gene in PK-15 Cell." Virus Research, vol. 227, 2017, pp. 143-149.
Ma T, Ouyang T, Ouyang H, et al. Porcine circovirus 2 proliferation can be enhanced by stably expressing porcine IL-2 gene in PK-15 cell. Virus Res. 2017;227:143-149.
Ma, T., Ouyang, T., Ouyang, H., Chen, F., Peng, Z., Chen, X., Pang, D., & Ren, L. (2017). Porcine circovirus 2 proliferation can be enhanced by stably expressing porcine IL-2 gene in PK-15 cell. Virus Research, 227, 143-149. https://doi.org/10.1016/j.virusres.2016.10.006
Ma T, et al. Porcine Circovirus 2 Proliferation Can Be Enhanced By Stably Expressing Porcine IL-2 Gene in PK-15 Cell. Virus Res. 2017 01 2;227:143-149. PubMed PMID: 27760373.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Porcine circovirus 2 proliferation can be enhanced by stably expressing porcine IL-2 gene in PK-15 cell. AU - Ma,Teng, AU - Ouyang,Ting, AU - Ouyang,Hongsheng, AU - Chen,Fuwang, AU - Peng,Zhiyuan, AU - Chen,Xinrong, AU - Pang,Daxin, AU - Ren,Linzhu, Y1 - 2016/10/17/ PY - 2016/09/01/received PY - 2016/10/12/revised PY - 2016/10/13/accepted PY - 2016/11/5/pubmed PY - 2017/12/13/medline PY - 2016/11/7/entrez KW - Interleukin-2 (IL-2) KW - Porcine circovirus 2 (PCV2) KW - Proliferation SP - 143 EP - 149 JF - Virus research JO - Virus Res. VL - 227 N2 - Porcine circovirus 2 (PCV2) is the causative agent of porcine circovirus diseases and porcine circovirus-associated diseases (PCVD/PCVAD), which are widely present in every major swine farm. However, lower propagation rate of PCV2 in vitro seriously hindered the production of PCV2 vaccine. Previously, we found that interleukin-2 (IL-2) can increase PCV2 yield in vitro. In the present study, porcine IL-2 gene was amplified and stably transfected into PK-15 cells. The results demonstrated that PCV2 proliferation can be significantly enhanced in cells stably expressing porcine IL-2 gene, suggesting that porcine IL-2 contributes to proliferation of PCV2. These results indicated that cells overexpressing porcine IL-2 gene can be used as a promising cell line for vaccine development of PCV2. SN - 1872-7492 UR - https://www.unboundmedicine.com/medline/citation/27760373/Porcine_circovirus_2_proliferation_can_be_enhanced_by_stably_expressing_porcine_IL_2_gene_in_PK_15_cell_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0168-1702(16)30559-7 DB - PRIME DP - Unbound Medicine ER -