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Nerve Growth Factor Protects Against Alcohol-Induced Neurotoxicity in PC12 Cells via PI3K/Akt/mTOR Pathway.
Alcohol Alcohol. 2017 Jan; 52(1):12-18.AA

Abstract

AIMS

To study whether autophagy participates in the neuroprotective effect of nerve growth factor (NGF) on neurons treated with alcohol.

METHODS

The autophagy-related markers were used to explore the role of autophagy in PC12 cells exposed to alcohol or pre-incubated with NGF before initiating the treatment with alcohol (100 mM; 6 h). PC12 cells were pre-incubated with 3-methyladenine (3-MA) (10 mM; 1 h) or rapamycin (100 nM; 1 h) before co-incubated with alcohol (100 mM; 6 h) in order to investigate the relationship between apoptosis and autophagy. PC12 cells were pre-incubated with LY294002 (50 μM; 30 min) before co-incubated with NGF and alcohol in order to analyze the protein expression of PI3K/Akt/mTOR pathway via western blotting.

RESULT

By methylthiazoltetrazolium, western blotting and flow cytometry assays, we found that cell viability decreased in a dose- and time-dependent manner after treatment with alcohol in PC12 cells. As cells were exposed to alcohol, the levels of LC3-II proteins became elevated, likewise, pre-treatment with 3-methyladenine (3-MA, an autophagic inhibitor) or rapamycin (an autophagic inducer) resulted in an increased or decreased percentage of apoptosis in contrast to other alcohol-treated groups, respectively. NGF markedly increased LC3-II production after treatment with alcohol in a dose-dependent manner. Moreover, NGF remarkably attenuated the phosphorylation effect of alcohol exposure on PI3K/Akt/mTOR pathway, which was suppressed by LY294002 (Akt inhibitor).

CONCLUSIONS

NGF protects against alcohol-induced neurotoxicity via PI3K/Akt/mTOR pathway.

SHORT SUMMARY

In this study, we chose the PC12 cell line as a neuronal model, and our results demonstrate that nerve growth factor can induce autophagy with the neuroprotective effect and regulatory mechanisms of alcohol-induced autophagy in PC12 cells.

Authors+Show Affiliations

The First Affiliated Hospital of Harbin Medical University, 23 Youzheng Street, Nangang District, Harbin, Heilongjiang 150001, PR China.School of Life Science and Technology, Harbin Institute of Technology, 2 Yikuang Street, Nangang District, Harbin, Heilongjiang 150080, PR China.The First Affiliated Hospital of Harbin Medical University, 23 Youzheng Street, Nangang District, Harbin, Heilongjiang 150001, PR China.School of Life Science and Technology, Harbin Institute of Technology, 2 Yikuang Street, Nangang District, Harbin, Heilongjiang 150080, PR China.School of Life Science and Technology, Harbin Institute of Technology, 2 Yikuang Street, Nangang District, Harbin, Heilongjiang 150080, PR China.The First Affiliated Hospital of Harbin Medical University, 23 Youzheng Street, Nangang District, Harbin, Heilongjiang 150001, PR China hujian0451@163.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27760741

Citation

Liu, Liping, et al. "Nerve Growth Factor Protects Against Alcohol-Induced Neurotoxicity in PC12 Cells Via PI3K/Akt/mTOR Pathway." Alcohol and Alcoholism (Oxford, Oxfordshire), vol. 52, no. 1, 2017, pp. 12-18.
Liu L, Sun T, Xin F, et al. Nerve Growth Factor Protects Against Alcohol-Induced Neurotoxicity in PC12 Cells via PI3K/Akt/mTOR Pathway. Alcohol Alcohol. 2017;52(1):12-18.
Liu, L., Sun, T., Xin, F., Cui, W., Guo, J., & Hu, J. (2017). Nerve Growth Factor Protects Against Alcohol-Induced Neurotoxicity in PC12 Cells via PI3K/Akt/mTOR Pathway. Alcohol and Alcoholism (Oxford, Oxfordshire), 52(1), 12-18. https://doi.org/10.1093/alcalc/agw077
Liu L, et al. Nerve Growth Factor Protects Against Alcohol-Induced Neurotoxicity in PC12 Cells Via PI3K/Akt/mTOR Pathway. Alcohol Alcohol. 2017;52(1):12-18. PubMed PMID: 27760741.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Nerve Growth Factor Protects Against Alcohol-Induced Neurotoxicity in PC12 Cells via PI3K/Akt/mTOR Pathway. AU - Liu,Liping, AU - Sun,Tiedong, AU - Xin,Feng, AU - Cui,Wei, AU - Guo,Jianquan, AU - Hu,Jian, Y1 - 2016/10/19/ PY - 2016/03/01/received PY - 2016/07/22/revised PY - 2016/09/22/accepted PY - 2016/11/1/pubmed PY - 2017/1/24/medline PY - 2016/11/1/entrez SP - 12 EP - 18 JF - Alcohol and alcoholism (Oxford, Oxfordshire) JO - Alcohol Alcohol VL - 52 IS - 1 N2 - AIMS: To study whether autophagy participates in the neuroprotective effect of nerve growth factor (NGF) on neurons treated with alcohol. METHODS: The autophagy-related markers were used to explore the role of autophagy in PC12 cells exposed to alcohol or pre-incubated with NGF before initiating the treatment with alcohol (100 mM; 6 h). PC12 cells were pre-incubated with 3-methyladenine (3-MA) (10 mM; 1 h) or rapamycin (100 nM; 1 h) before co-incubated with alcohol (100 mM; 6 h) in order to investigate the relationship between apoptosis and autophagy. PC12 cells were pre-incubated with LY294002 (50 μM; 30 min) before co-incubated with NGF and alcohol in order to analyze the protein expression of PI3K/Akt/mTOR pathway via western blotting. RESULT: By methylthiazoltetrazolium, western blotting and flow cytometry assays, we found that cell viability decreased in a dose- and time-dependent manner after treatment with alcohol in PC12 cells. As cells were exposed to alcohol, the levels of LC3-II proteins became elevated, likewise, pre-treatment with 3-methyladenine (3-MA, an autophagic inhibitor) or rapamycin (an autophagic inducer) resulted in an increased or decreased percentage of apoptosis in contrast to other alcohol-treated groups, respectively. NGF markedly increased LC3-II production after treatment with alcohol in a dose-dependent manner. Moreover, NGF remarkably attenuated the phosphorylation effect of alcohol exposure on PI3K/Akt/mTOR pathway, which was suppressed by LY294002 (Akt inhibitor). CONCLUSIONS: NGF protects against alcohol-induced neurotoxicity via PI3K/Akt/mTOR pathway. SHORT SUMMARY: In this study, we chose the PC12 cell line as a neuronal model, and our results demonstrate that nerve growth factor can induce autophagy with the neuroprotective effect and regulatory mechanisms of alcohol-induced autophagy in PC12 cells. SN - 1464-3502 UR - https://www.unboundmedicine.com/medline/citation/27760741/Nerve_Growth_Factor_Protects_Against_Alcohol_Induced_Neurotoxicity_in_PC12_Cells_via_PI3K/Akt/mTOR_Pathway_ L2 - https://academic.oup.com/alcalc/article-lookup/doi/10.1093/alcalc/agw077 DB - PRIME DP - Unbound Medicine ER -