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Anti-stress effects of drinking green tea with lowered caffeine and enriched theanine, epigallocatechin and arginine on psychosocial stress induced adrenal hypertrophy in mice.
Phytomedicine 2016; 23(12):1365-1374P

Abstract

BACKGROUND

Theanine, an amino acid in tea, has significant anti-stress effects on animals and humans. However, the anti-stress effects of drinking green tea have not yet been elucidated.

HYPOTHESIS/PURPOSE

The present study aimed to explore anti-stress effects of green tea and roles of tea components in a mouse model of psychosocial stress.

STUDY DESIGN

We examined anti-stress effects of three types of green teas, theanine-rich "Gyokuro", standard "Sencha", and Sencha with lowered caffeine (low-caffeine green tea). Furthermore, the roles of tea components such as caffeine, catechins, and other amino acids in anti-stress effects were examined.

METHODS

To prepare low-caffeine green tea, plucked new tea leaves were treated with a hot-water spray. Mice were psychosocially stressed from a conflict among male mice under confrontational housing. Mice consumed each tea that was eluted with room temperature water ad libitum. As a marker for the stress response, adrenal hypertrophy was compared with mice that ingested water.

RESULTS

Caffeine was significantly lowered by spraying hot-water on tea leaves. While epigallocatechin gallate (EGCG) is the main catechin in tea leaves, epigallocatechin (EGC) was mainly infused into water at room temperature. Adrenal hypertrophy was significantly suppressed in mice that ingested theanine-rich and low-caffeine green tea that were eluted with water at room temperature. Caffeine and EGCG suppressed the anti-stress effects of theanine while EGC and arginine (Arg) retained these effects.

CONCLUSION

These results suggest that drinking green tea exhibits anti-stress effects, where theanine, EGC and Arg cooperatively abolish the counter-effect of caffeine and EGCG on psychosocial stress induced adrenal hypertrophy in mice.

Authors+Show Affiliations

Department of Neurophysiology, School of Pharmaceutical Sciences, University of Shizuoka, Shizuoka, Japan; Tea Science Center, Graduate Division of Nutritional and Environmental Sciences, University of Shizuoka, Shizuoka, Japan. Electronic address: unno@u-shizuoka-ken.ac.jp.Department of Neurophysiology, School of Pharmaceutical Sciences, University of Shizuoka, Shizuoka, Japan.Department of Neurophysiology, School of Pharmaceutical Sciences, University of Shizuoka, Shizuoka, Japan.Department of Neurophysiology, School of Pharmaceutical Sciences, University of Shizuoka, Shizuoka, Japan.Department of Functional Plant Physiology, Faculty of Agriculture, Shizuoka University, Shizuoka, Japan.Department of Functional Plant Physiology, Faculty of Agriculture, Shizuoka University, Shizuoka, Japan.Tea Science Center, Graduate Division of Nutritional and Environmental Sciences, University of Shizuoka, Shizuoka, Japan.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27765356

Citation

Unno, Keiko, et al. "Anti-stress Effects of Drinking Green Tea With Lowered Caffeine and Enriched Theanine, Epigallocatechin and Arginine On Psychosocial Stress Induced Adrenal Hypertrophy in Mice." Phytomedicine : International Journal of Phytotherapy and Phytopharmacology, vol. 23, no. 12, 2016, pp. 1365-1374.
Unno K, Hara A, Nakagawa A, et al. Anti-stress effects of drinking green tea with lowered caffeine and enriched theanine, epigallocatechin and arginine on psychosocial stress induced adrenal hypertrophy in mice. Phytomedicine. 2016;23(12):1365-1374.
Unno, K., Hara, A., Nakagawa, A., Iguchi, K., Ohshio, M., Morita, A., & Nakamura, Y. (2016). Anti-stress effects of drinking green tea with lowered caffeine and enriched theanine, epigallocatechin and arginine on psychosocial stress induced adrenal hypertrophy in mice. Phytomedicine : International Journal of Phytotherapy and Phytopharmacology, 23(12), pp. 1365-1374. doi:10.1016/j.phymed.2016.07.006.
Unno K, et al. Anti-stress Effects of Drinking Green Tea With Lowered Caffeine and Enriched Theanine, Epigallocatechin and Arginine On Psychosocial Stress Induced Adrenal Hypertrophy in Mice. Phytomedicine. 2016 Nov 15;23(12):1365-1374. PubMed PMID: 27765356.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Anti-stress effects of drinking green tea with lowered caffeine and enriched theanine, epigallocatechin and arginine on psychosocial stress induced adrenal hypertrophy in mice. AU - Unno,Keiko, AU - Hara,Ayane, AU - Nakagawa,Aimi, AU - Iguchi,Kazuaki, AU - Ohshio,Megumi, AU - Morita,Akio, AU - Nakamura,Yoriyuki, Y1 - 2016/07/25/ PY - 2016/01/04/received PY - 2016/07/13/revised PY - 2016/07/19/accepted PY - 2016/10/22/pubmed PY - 2017/5/10/medline PY - 2016/10/22/entrez KW - Anti-stress effect KW - Arginine KW - Caffeine KW - Epigallocatechin KW - Green tea KW - Theanine SP - 1365 EP - 1374 JF - Phytomedicine : international journal of phytotherapy and phytopharmacology JO - Phytomedicine VL - 23 IS - 12 N2 - BACKGROUND: Theanine, an amino acid in tea, has significant anti-stress effects on animals and humans. However, the anti-stress effects of drinking green tea have not yet been elucidated. HYPOTHESIS/PURPOSE: The present study aimed to explore anti-stress effects of green tea and roles of tea components in a mouse model of psychosocial stress. STUDY DESIGN: We examined anti-stress effects of three types of green teas, theanine-rich "Gyokuro", standard "Sencha", and Sencha with lowered caffeine (low-caffeine green tea). Furthermore, the roles of tea components such as caffeine, catechins, and other amino acids in anti-stress effects were examined. METHODS: To prepare low-caffeine green tea, plucked new tea leaves were treated with a hot-water spray. Mice were psychosocially stressed from a conflict among male mice under confrontational housing. Mice consumed each tea that was eluted with room temperature water ad libitum. As a marker for the stress response, adrenal hypertrophy was compared with mice that ingested water. RESULTS: Caffeine was significantly lowered by spraying hot-water on tea leaves. While epigallocatechin gallate (EGCG) is the main catechin in tea leaves, epigallocatechin (EGC) was mainly infused into water at room temperature. Adrenal hypertrophy was significantly suppressed in mice that ingested theanine-rich and low-caffeine green tea that were eluted with water at room temperature. Caffeine and EGCG suppressed the anti-stress effects of theanine while EGC and arginine (Arg) retained these effects. CONCLUSION: These results suggest that drinking green tea exhibits anti-stress effects, where theanine, EGC and Arg cooperatively abolish the counter-effect of caffeine and EGCG on psychosocial stress induced adrenal hypertrophy in mice. SN - 1618-095X UR - https://www.unboundmedicine.com/medline/citation/27765356/Anti_stress_effects_of_drinking_green_tea_with_lowered_caffeine_and_enriched_theanine_epigallocatechin_and_arginine_on_psychosocial_stress_induced_adrenal_hypertrophy_in_mice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0944-7113(16)30121-0 DB - PRIME DP - Unbound Medicine ER -