Tags

Type your tag names separated by a space and hit enter

Histopathological analysis of tilapia gills (Oreochromis niloticus Linnaeus, 1758) exposed to sugarcane vinasse.
Ecotoxicol Environ Saf. 2017 Jan; 135:319-326.EE

Abstract

Sugarcane vinasse is one of the main residues generated by the transformation of cane into ethanol. Because of the high organic content (COD), high biochemical oxygen demand (BOD), low pH, the large amount that this residue is generated (15l for every liter of ethanol produced) and their use as fertilizer on the sugarcane crop, this residue is potentially polluting to the soil ecossystem and by percolation to water ecossystem too. Thus, this study aimed to assess the toxicity of vinasse by analyzing Oreochromis niloticus gills exposed to different dilutions (1%, 2.5%, 5% and 10%) in two bioassays. The gills were collected, fixed and analyzed using ultra morphological, histological, and histochemical techniques. After exposure to the vinasse, a statistically significant reduction of the ridges present on the surface of pavimentous cells was observed in one of the bioassays; such structures are responsible for mucus retention, which helps to protect the tissue. In addition, an intumescence of the cells was observed in the treatments with vinasse as well as an increase in the amount of chloridric cells. Some striking tissue changes detected in the treatments were epithelial detachment and loss of integrity of secondary lamellae, causing their rupture and consequent hemorrhage. In the first bioassay, the amount of these changes was statistically significant at the 5% dilution, and the focus of hemorrhage was significant at all dilution ratios. In the second bioassay, the epithelial disorganization was statistically significant only at the 2.5% dilution of vinasse. Moreover, for both bioassays performed, a significant increase in mucous cells was observed when compared with the control. Our results demonstrate the toxic action of sugarcane vinasse, which caused histopathological changes in the exposed animals at all four dilution tested. This highlights the need for caution in the disposal of sugarcane vinasse on the soil, especially due to its capacity for being leached or percolated into water resources, which could seriously damage aquatic fauna.

Authors+Show Affiliations

UNESP (Universidade Estadual Paulista), Av. 24-A, 1515. Bela Vista, Rio Claro, SP, Brasil.UNESP (Universidade Estadual Paulista), Av. 24-A, 1515. Bela Vista, Rio Claro, SP, Brasil; Centro Universitário Hermínio Ometto (FHO-UNIARARAS), Av. Dr. João Maximiliano Baruto, 500. Jd. Universitário. Araras, SP, Brasil.UNESP (Universidade Estadual Paulista), Av. 24-A, 1515. Bela Vista, Rio Claro, SP, Brasil.UNESP (Universidade Estadual Paulista), Av. 24-A, 1515. Bela Vista, Rio Claro, SP, Brasil.UNESP (Universidade Estadual Paulista), Av. 24-A, 1515. Bela Vista, Rio Claro, SP, Brasil. Electronic address: fontanet@rc.unesp.br.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27770647

Citation

Correia, J E., et al. "Histopathological Analysis of Tilapia Gills (Oreochromis Niloticus Linnaeus, 1758) Exposed to Sugarcane Vinasse." Ecotoxicology and Environmental Safety, vol. 135, 2017, pp. 319-326.
Correia JE, Christofoletti CA, Marcato ACC, et al. Histopathological analysis of tilapia gills (Oreochromis niloticus Linnaeus, 1758) exposed to sugarcane vinasse. Ecotoxicol Environ Saf. 2017;135:319-326.
Correia, J. E., Christofoletti, C. A., Marcato, A. C. C., Marinho, J. F. U., & Fontanetti, C. S. (2017). Histopathological analysis of tilapia gills (Oreochromis niloticus Linnaeus, 1758) exposed to sugarcane vinasse. Ecotoxicology and Environmental Safety, 135, 319-326. https://doi.org/10.1016/j.ecoenv.2016.10.004
Correia JE, et al. Histopathological Analysis of Tilapia Gills (Oreochromis Niloticus Linnaeus, 1758) Exposed to Sugarcane Vinasse. Ecotoxicol Environ Saf. 2017;135:319-326. PubMed PMID: 27770647.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Histopathological analysis of tilapia gills (Oreochromis niloticus Linnaeus, 1758) exposed to sugarcane vinasse. AU - Correia,J E, AU - Christofoletti,C A, AU - Marcato,A C C, AU - Marinho,J F U, AU - Fontanetti,C S, Y1 - 2016/10/19/ PY - 2015/09/25/received PY - 2016/09/19/revised PY - 2016/10/05/accepted PY - 2016/10/23/pubmed PY - 2017/1/24/medline PY - 2016/10/23/entrez KW - Aquatic toxicity KW - Histochemical KW - Histology KW - Stillage KW - Ultra-morphology SP - 319 EP - 326 JF - Ecotoxicology and environmental safety JO - Ecotoxicol. Environ. Saf. VL - 135 N2 - Sugarcane vinasse is one of the main residues generated by the transformation of cane into ethanol. Because of the high organic content (COD), high biochemical oxygen demand (BOD), low pH, the large amount that this residue is generated (15l for every liter of ethanol produced) and their use as fertilizer on the sugarcane crop, this residue is potentially polluting to the soil ecossystem and by percolation to water ecossystem too. Thus, this study aimed to assess the toxicity of vinasse by analyzing Oreochromis niloticus gills exposed to different dilutions (1%, 2.5%, 5% and 10%) in two bioassays. The gills were collected, fixed and analyzed using ultra morphological, histological, and histochemical techniques. After exposure to the vinasse, a statistically significant reduction of the ridges present on the surface of pavimentous cells was observed in one of the bioassays; such structures are responsible for mucus retention, which helps to protect the tissue. In addition, an intumescence of the cells was observed in the treatments with vinasse as well as an increase in the amount of chloridric cells. Some striking tissue changes detected in the treatments were epithelial detachment and loss of integrity of secondary lamellae, causing their rupture and consequent hemorrhage. In the first bioassay, the amount of these changes was statistically significant at the 5% dilution, and the focus of hemorrhage was significant at all dilution ratios. In the second bioassay, the epithelial disorganization was statistically significant only at the 2.5% dilution of vinasse. Moreover, for both bioassays performed, a significant increase in mucous cells was observed when compared with the control. Our results demonstrate the toxic action of sugarcane vinasse, which caused histopathological changes in the exposed animals at all four dilution tested. This highlights the need for caution in the disposal of sugarcane vinasse on the soil, especially due to its capacity for being leached or percolated into water resources, which could seriously damage aquatic fauna. SN - 1090-2414 UR - https://www.unboundmedicine.com/medline/citation/27770647/Histopathological_analysis_of_tilapia_gills_(Oreochromis_niloticus_Linnaeus,_1758)_exposed_to_sugarcane_vinasse L2 - https://linkinghub.elsevier.com/retrieve/pii/S0147-6513(16)30405-5 DB - PRIME DP - Unbound Medicine ER -