Mapping of novel powdery mildew resistance gene(s) from Agropyron cristatum chromosome 2P.Theor Appl Genet. 2017 Jan; 130(1):109-121.TA
Li, Huanhuan, et al. "Mapping of Novel Powdery Mildew Resistance Gene(s) From Agropyron Cristatum Chromosome 2P." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 130, no. 1, 2017, pp. 109-121.
Li H, Jiang B, Wang J, et al. Mapping of novel powdery mildew resistance gene(s) from Agropyron cristatum chromosome 2P. Theor Appl Genet. 2017;130(1):109-121.
Li, H., Jiang, B., Wang, J., Lu, Y., Zhang, J., Pan, C., Yang, X., Li, X., Liu, W., & Li, L. (2017). Mapping of novel powdery mildew resistance gene(s) from Agropyron cristatum chromosome 2P. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 130(1), 109-121. https://doi.org/10.1007/s00122-016-2797-9
Li H, et al. Mapping of Novel Powdery Mildew Resistance Gene(s) From Agropyron Cristatum Chromosome 2P. Theor Appl Genet. 2017;130(1):109-121. PubMed PMID: 27771744.
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TY - JOUR T1 - Mapping of novel powdery mildew resistance gene(s) from Agropyron cristatum chromosome 2P. AU - Li,Huanhuan, AU - Jiang,Bo, AU - Wang,Jingchang, AU - Lu,Yuqing, AU - Zhang,Jinpeng, AU - Pan,Cuili, AU - Yang,Xinming, AU - Li,Xiuquan, AU - Liu,Weihua, AU - Li,Lihui, Y1 - 2016/10/22/ PY - 2016/06/28/received PY - 2016/09/15/accepted PY - 2016/10/25/pubmed PY - 2017/2/14/medline PY - 2016/10/25/entrez SP - 109 EP - 121 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 130 IS - 1 N2 - KEY MESSAGE: A physical map of Agropyron cristatum 2P chromosome was constructed for the first time and the novel powdery mildew resistance gene(s) from chromosome 2P was(were) also mapped. Agropyron cristatum (L.) Gaertn. (2n = 28, PPPP), a wild relative of common wheat, is highly resistant to powdery mildew. Previous studies showed that wheat-A. cristatum 2P disomic addition line II-9-3 displayed high resistance to powdery mildew, and the resistance was attributable to A. cristatum chromosome 2P. To utilize and physically map the powdery mildew resistance gene(s), 15 wheat-A. cristatum 2P translocation lines and three A. cristatum 2P deletion lines with different chromosomal segment sizes, obtained from II-9-3 using 60Co-γ ray irradiation, were characterized using cytogenetic and molecular marker analysis. A. cristatum 2P chromosomal segments in the translocations were translocated to different wheat chromosomes, including 1A, 4A, 5A, 6A, 7A, 1B, 2B, 3B, 7B, 3D, 4D, and 6D. A physical map of the 2P chromosome was constructed with 82 STS markers, consisting of nine bins with 34 markers on 2PS and eight bins with 48 markers on 2PL. The BC1F2 populations of seven wheat-A. cristatum 2P translocation lines (2PT-3, 2PT-4, 2PT-5, 2PT-6, 2PT-8, 2PT-9, and 2PT-10) were developed by self-pollination, tested with powdery mildew and genotyped with 2P-specific STS markers. From these results, the gene(s) conferring powdery mildew resistance was(were) located on 2PL bin FL 0.66-0.86 and 19 2P-specific markers were identified in this bin. Moreover, two new powdery mildew-resistant translocation lines (2PT-4 and 2PT-5) with small 2PL chromosome segments were obtained. The newly developed wheat lines with powdery mildew resistance and the closely linked molecular markers will be valuable for wheat disease breeding in the future. SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/27771744/Mapping_of_novel_powdery_mildew_resistance_gene_s__from_Agropyron_cristatum_chromosome_2P_ DB - PRIME DP - Unbound Medicine ER -