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The role of drug efflux pumps in Malassezia pachydermatis and Malassezia furfur defence against azoles.
Mycoses. 2017 Mar; 60(3):178-182.M

Abstract

This study aims to evaluate the effect of efflux pump modulators (EPMs) on the minimal inhibitory concentration (MIC) of fluconazole (FLZ) and voriconazole (VOR) in Malassezia furfur and Malassezia pachydermatis. The in vitro efficacy of azoles, in combination with EPMs (ie haloperidol-HAL, promethazine-PTZ and cyclosporine A-CYS), against 21 M. furfur from bloodstream infection patients and 14 M. pachydermatis from the skin of dogs with dermatitis, was assessed using a broth microdilution chequerboard analysis. Data were analysed using the model-fractional inhibitory concentration index (FICI) method. The MIC of FLZ and VOR of Malassezia spp. decreased in the presence of sub-inhibitory concentrations of HAL and/or PTZ. The synergic effect was observed only in strains with FLZ MIC≥128 μg/mL for M. furfur, FLZ MIC≥64 μg/mL for M. pachydermatis and VOR MIC≥4 μg/mL in both Malassezia spp. These results suggest that the drug efflux pumps are involved as defence mechanisms to azole drugs in Malassezia yeast. The synergism might be related to an increased expression of efflux pump genes, eventually resulting in azole resistance phenomena. Finally, the above FLZ and VOR MIC values might be considered the cut-off to discriminate susceptible and resistant strains.

Authors+Show Affiliations

Dipartimento di Medicina Veterinaria, Università degli Studi di Bari "Aldo Moro", Bari, Italy.Dipartimento di Medicina Veterinaria, Università degli Studi di Bari "Aldo Moro", Bari, Italy.Dipartimento di Medicina Veterinaria, Università degli Studi di Bari "Aldo Moro", Bari, Italy.Dipartimento di Medicina Veterinaria, Università degli Studi di Bari "Aldo Moro", Bari, Italy.Dipartimento di Medicina Veterinaria, Università degli Studi di Bari "Aldo Moro", Bari, Italy.

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

27774659

Citation

Iatta, Roberta, et al. "The Role of Drug Efflux Pumps in Malassezia Pachydermatis and Malassezia Furfur Defence Against Azoles." Mycoses, vol. 60, no. 3, 2017, pp. 178-182.
Iatta R, Puttilli MR, Immediato D, et al. The role of drug efflux pumps in Malassezia pachydermatis and Malassezia furfur defence against azoles. Mycoses. 2017;60(3):178-182.
Iatta, R., Puttilli, M. R., Immediato, D., Otranto, D., & Cafarchia, C. (2017). The role of drug efflux pumps in Malassezia pachydermatis and Malassezia furfur defence against azoles. Mycoses, 60(3), 178-182. https://doi.org/10.1111/myc.12577
Iatta R, et al. The Role of Drug Efflux Pumps in Malassezia Pachydermatis and Malassezia Furfur Defence Against Azoles. Mycoses. 2017;60(3):178-182. PubMed PMID: 27774659.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The role of drug efflux pumps in Malassezia pachydermatis and Malassezia furfur defence against azoles. AU - Iatta,Roberta, AU - Puttilli,Maria Rita, AU - Immediato,Davide, AU - Otranto,Domenico, AU - Cafarchia,Claudia, Y1 - 2016/10/24/ PY - 2016/05/30/received PY - 2016/08/11/revised PY - 2016/09/22/accepted PY - 2016/10/25/pubmed PY - 2017/3/24/medline PY - 2016/10/25/entrez KW - Malassezia furfur KW - Malassezia pachydermatis KW - azoles KW - efflux pump inhibitors KW - haloperidol KW - promethazine SP - 178 EP - 182 JF - Mycoses JO - Mycoses VL - 60 IS - 3 N2 - This study aims to evaluate the effect of efflux pump modulators (EPMs) on the minimal inhibitory concentration (MIC) of fluconazole (FLZ) and voriconazole (VOR) in Malassezia furfur and Malassezia pachydermatis. The in vitro efficacy of azoles, in combination with EPMs (ie haloperidol-HAL, promethazine-PTZ and cyclosporine A-CYS), against 21 M. furfur from bloodstream infection patients and 14 M. pachydermatis from the skin of dogs with dermatitis, was assessed using a broth microdilution chequerboard analysis. Data were analysed using the model-fractional inhibitory concentration index (FICI) method. The MIC of FLZ and VOR of Malassezia spp. decreased in the presence of sub-inhibitory concentrations of HAL and/or PTZ. The synergic effect was observed only in strains with FLZ MIC≥128 μg/mL for M. furfur, FLZ MIC≥64 μg/mL for M. pachydermatis and VOR MIC≥4 μg/mL in both Malassezia spp. These results suggest that the drug efflux pumps are involved as defence mechanisms to azole drugs in Malassezia yeast. The synergism might be related to an increased expression of efflux pump genes, eventually resulting in azole resistance phenomena. Finally, the above FLZ and VOR MIC values might be considered the cut-off to discriminate susceptible and resistant strains. SN - 1439-0507 UR - https://www.unboundmedicine.com/medline/citation/27774659/The_role_of_drug_efflux_pumps_in_Malassezia_pachydermatis_and_Malassezia_furfur_defence_against_azoles_ L2 - https://doi.org/10.1111/myc.12577 DB - PRIME DP - Unbound Medicine ER -