Tags

Type your tag names separated by a space and hit enter

Expression of ribosomopathy genes during Xenopus tropicalis embryogenesis.
BMC Dev Biol 2016; 16(1):38BD

Abstract

BACKGROUND

Because ribosomes are ubiquitously required for protein production, it was long assumed that any inherited defect in ribosome manufacture would be embryonically lethal. However, several human congenital diseases have been found to be associated with mutations in ribosome biogenesis factors. Surprisingly, despite the global requirement for ribosomes, these "ribosomopathies" are characterized by distinct and tissue specific phenotypes. The reasons for such tissue proclivity in ribosomopathies remain mysterious but may include differential expression of ribosome biogenesis factors in distinct tissues.

METHODS

Here we use in situ hybridization of labeled antisense mRNA probes and ultra high temporal resolution RNA-Seq data to examine and compare expression of 13 disease associated ribosome biogenesis factors at six key stages in Xenopus tropicalis development.

RESULTS

Rather than being ubiquitously expressed during development, mRNAs of all examined ribosome biogenesis factors were highly enriched in specific tissues, including the cranial neural crest and ventral blood islands. Interestingly, expression of ribosome biogenesis factors demonstrates clear differences in timing, transcript number and tissue localization.

CONCLUSION

Ribosome biogenesis factor expression is more spatiotemporally regulated during embryonic development than previously expected and correlates closely with many of the common ribosomopathy phenotypes. Our findings provide information on the dynamic use of ribosome production machinery components during development and advance our understanding of their roles in disease.

Authors+Show Affiliations

Program in Vertebrate Developmental Biology, Departments of Pediatrics and Genetics, Yale University School of Medicine, 333 Cedar Street, New Haven, CT, 06510, USA.The Francis Crick Institute, Mill Hill Laboratory, The Ridgeway, London, NW7 1AA, UK.Departments of Genetics, Molecular Biophysics and Biochemistry, and Therapeutic Radiology, Yale University School of Medicine, New Haven, CT, 06520, USA.Program in Vertebrate Developmental Biology, Departments of Pediatrics and Genetics, Yale University School of Medicine, 333 Cedar Street, New Haven, CT, 06510, USA.Program in Vertebrate Developmental Biology, Departments of Pediatrics and Genetics, Yale University School of Medicine, 333 Cedar Street, New Haven, CT, 06510, USA. J.Griffin@yale.edu.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

27784267

Citation

Robson, Andrew, et al. "Expression of Ribosomopathy Genes During Xenopus Tropicalis Embryogenesis." BMC Developmental Biology, vol. 16, no. 1, 2016, p. 38.
Robson A, Owens ND, Baserga SJ, et al. Expression of ribosomopathy genes during Xenopus tropicalis embryogenesis. BMC Dev Biol. 2016;16(1):38.
Robson, A., Owens, N. D., Baserga, S. J., Khokha, M. K., & Griffin, J. N. (2016). Expression of ribosomopathy genes during Xenopus tropicalis embryogenesis. BMC Developmental Biology, 16(1), p. 38.
Robson A, et al. Expression of Ribosomopathy Genes During Xenopus Tropicalis Embryogenesis. BMC Dev Biol. 2016 10 26;16(1):38. PubMed PMID: 27784267.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression of ribosomopathy genes during Xenopus tropicalis embryogenesis. AU - Robson,Andrew, AU - Owens,Nick D L, AU - Baserga,Susan J, AU - Khokha,Mustafa K, AU - Griffin,John N, Y1 - 2016/10/26/ PY - 2016/05/26/received PY - 2016/10/07/accepted PY - 2016/10/28/pubmed PY - 2017/9/7/medline PY - 2016/10/28/entrez KW - Development KW - Diamond-Blackfan anemia KW - North American Childhood Cirrhosis KW - RPL KW - RPS KW - Ribosome KW - Ribosome biogenesis KW - Ribosomopathy KW - TCOF1 KW - UTP KW - Xenopus SP - 38 EP - 38 JF - BMC developmental biology JO - BMC Dev. Biol. VL - 16 IS - 1 N2 - BACKGROUND: Because ribosomes are ubiquitously required for protein production, it was long assumed that any inherited defect in ribosome manufacture would be embryonically lethal. However, several human congenital diseases have been found to be associated with mutations in ribosome biogenesis factors. Surprisingly, despite the global requirement for ribosomes, these "ribosomopathies" are characterized by distinct and tissue specific phenotypes. The reasons for such tissue proclivity in ribosomopathies remain mysterious but may include differential expression of ribosome biogenesis factors in distinct tissues. METHODS: Here we use in situ hybridization of labeled antisense mRNA probes and ultra high temporal resolution RNA-Seq data to examine and compare expression of 13 disease associated ribosome biogenesis factors at six key stages in Xenopus tropicalis development. RESULTS: Rather than being ubiquitously expressed during development, mRNAs of all examined ribosome biogenesis factors were highly enriched in specific tissues, including the cranial neural crest and ventral blood islands. Interestingly, expression of ribosome biogenesis factors demonstrates clear differences in timing, transcript number and tissue localization. CONCLUSION: Ribosome biogenesis factor expression is more spatiotemporally regulated during embryonic development than previously expected and correlates closely with many of the common ribosomopathy phenotypes. Our findings provide information on the dynamic use of ribosome production machinery components during development and advance our understanding of their roles in disease. SN - 1471-213X UR - https://www.unboundmedicine.com/medline/citation/27784267/Expression_of_ribosomopathy_genes_during_Xenopus_tropicalis_embryogenesis_ L2 - https://bmcdevbiol.biomedcentral.com/articles/10.1186/s12861-016-0138-5 DB - PRIME DP - Unbound Medicine ER -