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Bacteriological and molecular studies of Clostridium perfringens infections in newly born calves.
Trop Anim Health Prod 2017; 49(1):201-205TA

Abstract

Clostridium perfringens is considered one of the important causes of calf diarrhea. Two hundred and twenty-seven clinical samples from newly born and dead diarrheic calves were examined bacteriologically and by PCR. Bacterial culture identified C. perfringens in 168 of 227 samples. A total of 144 of these isolates were lecithinase positive, indicating C. perfringens Type A. In addition, 154 isolates were positive by alpha toxin encoding gene-PCR assay. This study showed high agreement between the results of bacteriology and multiplex PCR. The multiplex PCR typed all isolates that were typed as C. perfringens Type A through bacteriologic methods, but ten samples that were lecithinase negative were positive in the multiplex PCR. The study showed the highest occurrence of C. perfringens Type A isolations from calves during the winter and autumn compared with other seasons.

Authors+Show Affiliations

Department of Animal Medicine (Infectious Diseases), Faculty of Veterinary Medicine, Benha University, Benha, Egypt.Department of Animal Medicine (Infectious Diseases), Faculty of Veterinary Medicine, Suez Canal University, Ismailia, 41522, Egypt. melhaig@vet.suez.edu.eg.Animal health Research Institute, Dokki, Giza, Egypt.Faculty of Veterinary Medicine, Benha University, Benha, Egypt.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27785764

Citation

Selim, A M., et al. "Bacteriological and Molecular Studies of Clostridium Perfringens Infections in Newly Born Calves." Tropical Animal Health and Production, vol. 49, no. 1, 2017, pp. 201-205.
Selim AM, Elhaig MM, Zakaria I, et al. Bacteriological and molecular studies of Clostridium perfringens infections in newly born calves. Trop Anim Health Prod. 2017;49(1):201-205.
Selim, A. M., Elhaig, M. M., Zakaria, I., & Ali, A. (2017). Bacteriological and molecular studies of Clostridium perfringens infections in newly born calves. Tropical Animal Health and Production, 49(1), pp. 201-205. doi:10.1007/s11250-016-1181-8.
Selim AM, et al. Bacteriological and Molecular Studies of Clostridium Perfringens Infections in Newly Born Calves. Trop Anim Health Prod. 2017;49(1):201-205. PubMed PMID: 27785764.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Bacteriological and molecular studies of Clostridium perfringens infections in newly born calves. AU - Selim,A M, AU - Elhaig,M M, AU - Zakaria,I, AU - Ali,A, Y1 - 2016/10/26/ PY - 2016/03/22/received PY - 2016/10/17/accepted PY - 2016/10/28/pubmed PY - 2017/7/29/medline PY - 2016/10/28/entrez KW - Bacteriology KW - Clostridium perfringens KW - Multiplex PCR KW - Seasonal prevalence SP - 201 EP - 205 JF - Tropical animal health and production JO - Trop Anim Health Prod VL - 49 IS - 1 N2 - Clostridium perfringens is considered one of the important causes of calf diarrhea. Two hundred and twenty-seven clinical samples from newly born and dead diarrheic calves were examined bacteriologically and by PCR. Bacterial culture identified C. perfringens in 168 of 227 samples. A total of 144 of these isolates were lecithinase positive, indicating C. perfringens Type A. In addition, 154 isolates were positive by alpha toxin encoding gene-PCR assay. This study showed high agreement between the results of bacteriology and multiplex PCR. The multiplex PCR typed all isolates that were typed as C. perfringens Type A through bacteriologic methods, but ten samples that were lecithinase negative were positive in the multiplex PCR. The study showed the highest occurrence of C. perfringens Type A isolations from calves during the winter and autumn compared with other seasons. SN - 1573-7438 UR - https://www.unboundmedicine.com/medline/citation/27785764/Bacteriological_and_molecular_studies_of_Clostridium_perfringens_infections_in_newly_born_calves L2 - https://dx.doi.org/10.1007/s11250-016-1181-8 DB - PRIME DP - Unbound Medicine ER -