Bacteriological and molecular studies of Clostridium perfringens infections in newly born calves.
Clostridium perfringens is considered one of the important causes of calf diarrhea. Two hundred and twenty-seven clinical samples from newly born and dead diarrheic calves were examined bacteriologically and by PCR. Bacterial culture identified C. perfringens in 168 of 227 samples. A total of 144 of these isolates were lecithinase positive, indicating C. perfringens Type A. In addition, 154 isolates were positive by alpha toxin encoding gene-PCR assay. This study showed high agreement between the results of bacteriology and multiplex PCR. The multiplex PCR typed all isolates that were typed as C. perfringens Type A through bacteriologic methods, but ten samples that were lecithinase negative were positive in the multiplex PCR. The study showed the highest occurrence of C. perfringens Type A isolations from calves during the winter and autumn compared with other seasons.
Department of Animal Medicine (Infectious Diseases), Faculty of Veterinary Medicine, Benha University, Benha, Egypt.,
Department of Animal Medicine (Infectious Diseases), Faculty of Veterinary Medicine, Suez Canal University, Ismailia, 41522, Egypt. email@example.com.,
Animal health Research Institute, Dokki, Giza, Egypt.
Faculty of Veterinary Medicine, Benha University, Benha, Egypt.
Multiplex Polymerase Chain Reaction
Polymerase Chain Reaction
Pub Type(s)Journal Article