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The gene expression profile of non-cultured, highly purified human adipose tissue pericytes: Transcriptomic evidence that pericytes are stem cells in human adipose tissue.
Exp Cell Res 2016; 349(2):239-254EC

Abstract

Pericytes (PCs) are a subset of perivascular cells that can give rise to mesenchymal stromal cells (MSCs) when culture-expanded, and are postulated to give rise to MSC-like cells during tissue repair in vivo. PCs have been suggested to behave as stem cells (SCs) in situ in animal models, although evidence for this role in humans is lacking. Here, we analyzed the transcriptomes of highly purified, non-cultured adipose tissue (AT)-derived PCs (ATPCs) to detect gene expression changes that occur as they acquire MSC characteristics in vitro, and evaluated the hypothesis that human ATPCs exhibit a gene expression profile compatible with an AT SC phenotype. The results showed ATPCs are non-proliferative and express genes characteristic not only of PCs, but also of AT stem/progenitor cells. Additional analyses defined a gene expression signature for ATPCs, and revealed putative novel ATPC markers. Almost all AT stem/progenitor cell genes differentially expressed by ATPCs were not expressed by ATMSCs or culture-expanded ATPCs. Genes expressed by ATMSCs but not by ATPCs were also identified. These findings strengthen the hypothesis that PCs are SCs in vascularized tissues, highlight gene expression changes they undergo as they assume an MSC phenotype, and provide new insights into PC biology.

Authors+Show Affiliations

Center for Cell-Based Therapy (CEPID/FAPESP), Regional Center for Hemotherapy of Ribeirão Preto, University of São Paulo, Rua Tenente Catão Roxo 2501, 14051-140 Ribeirão Preto, SP, Brazil; Laboratory for Stem Cells and Tissue Engineering, PPGBioSaúde, Lutheran University of Brazil, Av. Farroupilha 8001, 92425-900 Canoas, RS, Brazil. Electronic address: lindolfomeirelles@gmail.com.Center for Cell-Based Therapy (CEPID/FAPESP), Regional Center for Hemotherapy of Ribeirão Preto, University of São Paulo, Rua Tenente Catão Roxo 2501, 14051-140 Ribeirão Preto, SP, Brazil.Center for Cell-Based Therapy (CEPID/FAPESP), Regional Center for Hemotherapy of Ribeirão Preto, University of São Paulo, Rua Tenente Catão Roxo 2501, 14051-140 Ribeirão Preto, SP, Brazil.Center for Cell-Based Therapy (CEPID/FAPESP), Regional Center for Hemotherapy of Ribeirão Preto, University of São Paulo, Rua Tenente Catão Roxo 2501, 14051-140 Ribeirão Preto, SP, Brazil.Laboratory of Large-Scale Functional Biology (LLSFBio), Regional Center for Hemotherapy of Ribeirão Preto, University of São Paulo, Rua Tenente Catão Roxo 2501, 14051-140 Ribeirão Preto, SP, Brazil.Laboratory of Large-Scale Functional Biology (LLSFBio), Regional Center for Hemotherapy of Ribeirão Preto, University of São Paulo, Rua Tenente Catão Roxo 2501, 14051-140 Ribeirão Preto, SP, Brazil.Center for Cell-Based Therapy (CEPID/FAPESP), Regional Center for Hemotherapy of Ribeirão Preto, University of São Paulo, Rua Tenente Catão Roxo 2501, 14051-140 Ribeirão Preto, SP, Brazil; Department of Genetics, Ribeirão Preto Medical School, University of São Paulo, Av. Bandeirantes 3900, 14049-900 Ribeirão Preto, SP, Brazil.Center for Cell-Based Therapy (CEPID/FAPESP), Regional Center for Hemotherapy of Ribeirão Preto, University of São Paulo, Rua Tenente Catão Roxo 2501, 14051-140 Ribeirão Preto, SP, Brazil.Center for Cell-Based Therapy (CEPID/FAPESP), Regional Center for Hemotherapy of Ribeirão Preto, University of São Paulo, Rua Tenente Catão Roxo 2501, 14051-140 Ribeirão Preto, SP, Brazil; Department of Clinical Medicine, Ribeirão Preto Medical School, University of São Paulo, Av. Bandeirantes 3900, 14049-900 Ribeirão Preto, SP, Brazil.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27789253

Citation

da Silva Meirelles, Lindolfo, et al. "The Gene Expression Profile of Non-cultured, Highly Purified Human Adipose Tissue Pericytes: Transcriptomic Evidence That Pericytes Are Stem Cells in Human Adipose Tissue." Experimental Cell Research, vol. 349, no. 2, 2016, pp. 239-254.
da Silva Meirelles L, de Deus Wagatsuma VM, Malta TM, et al. The gene expression profile of non-cultured, highly purified human adipose tissue pericytes: Transcriptomic evidence that pericytes are stem cells in human adipose tissue. Exp Cell Res. 2016;349(2):239-254.
da Silva Meirelles, L., de Deus Wagatsuma, V. M., Malta, T. M., Bonini Palma, P. V., Araújo, A. G., Panepucci, R. A., ... Covas, D. T. (2016). The gene expression profile of non-cultured, highly purified human adipose tissue pericytes: Transcriptomic evidence that pericytes are stem cells in human adipose tissue. Experimental Cell Research, 349(2), pp. 239-254. doi:10.1016/j.yexcr.2016.10.017.
da Silva Meirelles L, et al. The Gene Expression Profile of Non-cultured, Highly Purified Human Adipose Tissue Pericytes: Transcriptomic Evidence That Pericytes Are Stem Cells in Human Adipose Tissue. Exp Cell Res. 2016 Dec 10;349(2):239-254. PubMed PMID: 27789253.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The gene expression profile of non-cultured, highly purified human adipose tissue pericytes: Transcriptomic evidence that pericytes are stem cells in human adipose tissue. AU - da Silva Meirelles,Lindolfo, AU - de Deus Wagatsuma,Virgínia Mara, AU - Malta,Tathiane Maistro, AU - Bonini Palma,Patrícia Viana, AU - Araújo,Amélia Goes, AU - Panepucci,Rodrigo Alexandre, AU - Silva,Wilson Araújo, AU - Kashima,Simone, AU - Covas,Dimas Tadeu, Y1 - 2016/10/24/ PY - 2016/06/24/received PY - 2016/10/20/revised PY - 2016/10/22/accepted PY - 2016/11/4/pubmed PY - 2017/5/17/medline PY - 2016/11/4/entrez KW - Adipose tissue KW - Gene expression KW - Mesenchymal stem cells KW - Mesenchymal stromal cells KW - Pericytes SP - 239 EP - 254 JF - Experimental cell research JO - Exp. Cell Res. VL - 349 IS - 2 N2 - Pericytes (PCs) are a subset of perivascular cells that can give rise to mesenchymal stromal cells (MSCs) when culture-expanded, and are postulated to give rise to MSC-like cells during tissue repair in vivo. PCs have been suggested to behave as stem cells (SCs) in situ in animal models, although evidence for this role in humans is lacking. Here, we analyzed the transcriptomes of highly purified, non-cultured adipose tissue (AT)-derived PCs (ATPCs) to detect gene expression changes that occur as they acquire MSC characteristics in vitro, and evaluated the hypothesis that human ATPCs exhibit a gene expression profile compatible with an AT SC phenotype. The results showed ATPCs are non-proliferative and express genes characteristic not only of PCs, but also of AT stem/progenitor cells. Additional analyses defined a gene expression signature for ATPCs, and revealed putative novel ATPC markers. Almost all AT stem/progenitor cell genes differentially expressed by ATPCs were not expressed by ATMSCs or culture-expanded ATPCs. Genes expressed by ATMSCs but not by ATPCs were also identified. These findings strengthen the hypothesis that PCs are SCs in vascularized tissues, highlight gene expression changes they undergo as they assume an MSC phenotype, and provide new insights into PC biology. SN - 1090-2422 UR - https://www.unboundmedicine.com/medline/citation/27789253/The_gene_expression_profile_of_non_cultured_highly_purified_human_adipose_tissue_pericytes:_Transcriptomic_evidence_that_pericytes_are_stem_cells_in_human_adipose_tissue_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-4827(16)30347-0 DB - PRIME DP - Unbound Medicine ER -