Tags

Type your tag names separated by a space and hit enter

Preparation of Pure and Stable Chitosan Nanofibers by Electrospinning in the Presence of Poly(ethylene oxide).
Int J Mol Sci. 2016 Oct 26; 17(11)IJ

Abstract

Electrospinning was employed to obtain chitosan nanofibers from blends of chitosans (CS) and poly(ethylene oxide) (PEO). Blends of chitosan (MW (weight-average molecular weight) = 102 kg/mol) and PEO (M (molecular weight) = 1000 kg/mol) were selected to optimize the electrospinning process parameters. The PEO powder was solubilized into chitosan solution at different weight ratios in 0.5 M acetic acid. The physicochemical changes of the nanofibers were determined by scanning electron microscopy (SEM), swelling capacity, and nuclear magnetic resonance (NMR) spectroscopy. For stabilization, the produced nanofibers were neutralized with K₂CO₃ in water or 70% ethanol/30% water as solvent. Subsequently, repeated washings with pure water were performed to extract PEO, potassium acetate and carbonate salts formed in the course of chitosan nanofiber purification. The increase of PEO content in the blend from 20 to 40 w% exhibited bead-free fibers with average diameters 85 ± 19 and 147 ± 28 nm, respectively. Their NMR analysis proved that PEO and the salts were nearly completely removed from the nanostructure of chitosan, demonstrating that the adopted strategy is successful for producing pure chitosan nanofibers. In addition, the nanofibers obtained after neutralization in ethanol-aqueous solution has better structural stability, at least for six months in aqueous solutions (phosphate buffer (PBS) or water).

Links

PMC Free PDF

Authors+Show Affiliations

Mengistu Lemma S
Laboratoire Rhéologie et Procédés (LRP), University of Grenoble Alpes-CNRS, 38000 Grenoble, France. solomonmengistu@gmail.com.
Bossard F
Laboratoire Rhéologie et Procédés (LRP), University of Grenoble Alpes-CNRS, 38000 Grenoble, France. frederic.bossard@ujf-grenoble.fr.
Rinaudo M
Biomaterials Applications, 6 Rue Lesdiguières, 38000 Grenoble, France. marguerite.rinaudo@sfr.fr.

MeSH

ChitosanElectrochemical TechniquesEquipment DesignNanofibersNanotechnologyPolyethylene GlycolsViscosity

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27792192

Citation

Mengistu Lemma, Solomon, et al. "Preparation of Pure and Stable Chitosan Nanofibers By Electrospinning in the Presence of Poly(ethylene Oxide)." International Journal of Molecular Sciences, vol. 17, no. 11, 2016.
Mengistu Lemma S, Bossard F, Rinaudo M. Preparation of Pure and Stable Chitosan Nanofibers by Electrospinning in the Presence of Poly(ethylene oxide). Int J Mol Sci. 2016;17(11).
Mengistu Lemma, S., Bossard, F., & Rinaudo, M. (2016). Preparation of Pure and Stable Chitosan Nanofibers by Electrospinning in the Presence of Poly(ethylene oxide). International Journal of Molecular Sciences, 17(11).
Mengistu Lemma S, Bossard F, Rinaudo M. Preparation of Pure and Stable Chitosan Nanofibers By Electrospinning in the Presence of Poly(ethylene Oxide). Int J Mol Sci. 2016 Oct 26;17(11) PubMed PMID: 27792192.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Preparation of Pure and Stable Chitosan Nanofibers by Electrospinning in the Presence of Poly(ethylene oxide). AU - Mengistu Lemma,Solomon, AU - Bossard,Frédéric, AU - Rinaudo,Marguerite, Y1 - 2016/10/26/ PY - 2016/09/22/received PY - 2016/10/18/revised PY - 2016/10/19/accepted PY - 2016/10/30/pubmed PY - 2017/4/14/medline PY - 2016/10/30/entrez KW - PEO KW - chitosan KW - electrospinning KW - nanofiber KW - stability JF - International journal of molecular sciences JO - Int J Mol Sci VL - 17 IS - 11 N2 - Electrospinning was employed to obtain chitosan nanofibers from blends of chitosans (CS) and poly(ethylene oxide) (PEO). Blends of chitosan (MW (weight-average molecular weight) = 102 kg/mol) and PEO (M (molecular weight) = 1000 kg/mol) were selected to optimize the electrospinning process parameters. The PEO powder was solubilized into chitosan solution at different weight ratios in 0.5 M acetic acid. The physicochemical changes of the nanofibers were determined by scanning electron microscopy (SEM), swelling capacity, and nuclear magnetic resonance (NMR) spectroscopy. For stabilization, the produced nanofibers were neutralized with K₂CO₃ in water or 70% ethanol/30% water as solvent. Subsequently, repeated washings with pure water were performed to extract PEO, potassium acetate and carbonate salts formed in the course of chitosan nanofiber purification. The increase of PEO content in the blend from 20 to 40 w% exhibited bead-free fibers with average diameters 85 ± 19 and 147 ± 28 nm, respectively. Their NMR analysis proved that PEO and the salts were nearly completely removed from the nanostructure of chitosan, demonstrating that the adopted strategy is successful for producing pure chitosan nanofibers. In addition, the nanofibers obtained after neutralization in ethanol-aqueous solution has better structural stability, at least for six months in aqueous solutions (phosphate buffer (PBS) or water). SN - 1422-0067 UR - https://www.unboundmedicine.com/medline/citation/27792192/Preparation_of_Pure_and_Stable_Chitosan_Nanofibers_by_Electrospinning_in_the_Presence_of_Poly_ethylene_oxide__ DB - PRIME DP - Unbound Medicine ER -