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Metabolic imidacloprid resistance in the brown planthopper, Nilaparvata lugens, relies on multiple P450 enzymes.
Insect Biochem Mol Biol. 2016 12; 79:50-56.IB

Abstract

Target insensitivity contributing to imidacloprid resistance in Nilaparvata lugens has been reported to occur either through point mutations or quantitative change in nicotinic acetylcholine receptors (nAChRs). However, the metabolic resistance, especially the enhanced detoxification by P450 enzymes, is the major mechanism in fields. From one field-originated N. lugens population, an imidacloprid resistant strain G25 and a susceptible counterpart S25 were obtained to analyze putative roles of P450s in imidacloprid resistance. Compared to S25, over-expression of twelve P450 genes was observed in G25, with ratios above 5.0-fold for CYP6AY1, CYP6ER1, CYP6CS1, CYP6CW1, CYP4CE1 and CYP425B1. RNAi against these genes in vivo and recombinant tests on the corresponding proteins in vitro revealed that four P450s, CYP6AY1, CYP6ER1, CYP4CE1 and CYP6CW1, played important roles in imidacloprid resistance. The importance of the four P450s was not equal at different stages of resistance development based on their over-expression levels, among which CYP6ER1 was important at all stages, and that the others might only contribute at certain stages. The results indicated that, to completely reflect roles of P450s in insecticide resistances, their over-expression in resistant individuals, expression changes at the stages of resistance development, and catalytic activities against insecticides should be considered. In this study, multiple P450s, CYP6AY1, CYP6ER1, CYP4CE1 and CYP6CW1, have proven to be important in imidacloprid resistance.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Zhang Y
Key Laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education), College of Plant Protection, Nanjing Agricultural University, Weigang 1, Nanjing 210095, China.
Yang Y
Key Laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education), College of Plant Protection, Nanjing Agricultural University, Weigang 1, Nanjing 210095, China.
Sun H
Key Laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education), College of Plant Protection, Nanjing Agricultural University, Weigang 1, Nanjing 210095, China.
Liu Z
Key Laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education), College of Plant Protection, Nanjing Agricultural University, Weigang 1, Nanjing 210095, China. Electronic address: liuzewen@njau.edu.cn.

MeSH

AnimalsCytochrome P-450 Enzyme SystemHemipteraImidazolesInsect ProteinsInsecticide ResistanceInsecticidesNeonicotinoidsNitro CompoundsNymphTranscriptome

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27793627

Citation

Zhang, Yixi, et al. "Metabolic Imidacloprid Resistance in the Brown Planthopper, Nilaparvata Lugens, Relies On Multiple P450 Enzymes." Insect Biochemistry and Molecular Biology, vol. 79, 2016, pp. 50-56.
Zhang Y, Yang Y, Sun H, et al. Metabolic imidacloprid resistance in the brown planthopper, Nilaparvata lugens, relies on multiple P450 enzymes. Insect Biochem Mol Biol. 2016;79:50-56.
Zhang, Y., Yang, Y., Sun, H., & Liu, Z. (2016). Metabolic imidacloprid resistance in the brown planthopper, Nilaparvata lugens, relies on multiple P450 enzymes. Insect Biochemistry and Molecular Biology, 79, 50-56. https://doi.org/10.1016/j.ibmb.2016.10.009
Zhang Y, et al. Metabolic Imidacloprid Resistance in the Brown Planthopper, Nilaparvata Lugens, Relies On Multiple P450 Enzymes. Insect Biochem Mol Biol. 2016;79:50-56. PubMed PMID: 27793627.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Metabolic imidacloprid resistance in the brown planthopper, Nilaparvata lugens, relies on multiple P450 enzymes. AU - Zhang,Yixi, AU - Yang,Yuanxue, AU - Sun,Huahua, AU - Liu,Zewen, Y1 - 2016/10/26/ PY - 2016/08/13/received PY - 2016/10/23/revised PY - 2016/10/24/accepted PY - 2016/10/30/pubmed PY - 2017/9/7/medline PY - 2016/11/5/entrez KW - Imidacloprid KW - Metabolic resistance KW - Nilaparvata lugens KW - P450s SP - 50 EP - 56 JF - Insect biochemistry and molecular biology JO - Insect Biochem Mol Biol VL - 79 N2 - Target insensitivity contributing to imidacloprid resistance in Nilaparvata lugens has been reported to occur either through point mutations or quantitative change in nicotinic acetylcholine receptors (nAChRs). However, the metabolic resistance, especially the enhanced detoxification by P450 enzymes, is the major mechanism in fields. From one field-originated N. lugens population, an imidacloprid resistant strain G25 and a susceptible counterpart S25 were obtained to analyze putative roles of P450s in imidacloprid resistance. Compared to S25, over-expression of twelve P450 genes was observed in G25, with ratios above 5.0-fold for CYP6AY1, CYP6ER1, CYP6CS1, CYP6CW1, CYP4CE1 and CYP425B1. RNAi against these genes in vivo and recombinant tests on the corresponding proteins in vitro revealed that four P450s, CYP6AY1, CYP6ER1, CYP4CE1 and CYP6CW1, played important roles in imidacloprid resistance. The importance of the four P450s was not equal at different stages of resistance development based on their over-expression levels, among which CYP6ER1 was important at all stages, and that the others might only contribute at certain stages. The results indicated that, to completely reflect roles of P450s in insecticide resistances, their over-expression in resistant individuals, expression changes at the stages of resistance development, and catalytic activities against insecticides should be considered. In this study, multiple P450s, CYP6AY1, CYP6ER1, CYP4CE1 and CYP6CW1, have proven to be important in imidacloprid resistance. SN - 1879-0240 UR - https://www.unboundmedicine.com/medline/citation/27793627/Metabolic_imidacloprid_resistance_in_the_brown_planthopper_Nilaparvata_lugens_relies_on_multiple_P450_enzymes_ DB - PRIME DP - Unbound Medicine ER -