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Effects of crude hydroalcoholic extract of Syzygium cumini (L.) Skeels leaves and continuous aerobic training in rats with diabetes induced by a high-fat diet and low doses of streptozotocin.
J Ethnopharmacol. 2016 Dec 24; 194:1012-1021.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

The leaves of Syzygium cumini (L.) or Skeels (Myrtaceae) are widely used in Brazilian folk medicine to treat diabetes.

AIM OF THE STUDY

The present study evaluated the functional capacity, biochemical parameters, oxidative stress and DNA damage from eight weeks of intervention with a crude hydroalcoholic extract of S. cumini leaves (EBH) and continuous aerobic training (TAC) in diabetic (D) rats.

MATERIALS AND METHODS

A hydroalcoholic (50%) extract was prepared by ultrasound and phytochemical parameters (total phenols, total tannins and myricetin content) were analyzed. Thirty-seven male Wistar rats were divided into five groups: normoglycemic controls (CONT), diabetic controls (D-CONT), diabetics treated with extract (D+EBH), trained diabetic (D+TAC) and diabetics treated with extract and trained (D+EBH+TAC). Functional capacity was assessed with a maximum exercise capacity test; biochemical parameters with enzymatic kits; oxidative stress by superoxide dismutase (SOD), catalase (CAT), thiobarbituric acid reactive substances (TBARS) and oxidized dichlorofluorescein (DCF), and the DNA damage by the comet assay.

RESULTS

The D+TAC and D+EBH+TAC groups showed better functional capacity at the end of interventions. The D+EBH group showed glucose and triglyceride reduction, lowest DNA damage index in the blood, liver, kidney, heart, lung and gastrocnemius muscle, improved SOD levels in the liver, kidney and lung, improved CAT levels in the kidney and lower lipid peroxidation in all tissues studied, compared to the D-CONT group. The exercise (D+TAC) was effective in reducing triglycerides, improving SOD levels in the lung, reducing lipid peroxidation in all tissues studied and reducing the DCF oxidation in the kidney, in addition to protecting against DNA damage in the blood and heart. However, the additive effect of the intervention protocols when combined (EBH+TAC) was observed only in improving the gastrocnemius SOD levels. The phytochemical analyses showed a high content of phenols and the presence of myricetin glycosides.

CONCLUSION

The findings in this study suggest a crude hydroalcoholic extract of S. cumini leaves has potential hypoglycemic, hypolipidemic and protective properties acting against oxidative stress and against DNA damage, probably due to its phenols and myricetin glycoside content and the antioxidant properties of these constituents. Moreover, exercise was suggested to have beneficial effects on diabetes, improving functional capacity, ameliorating blood triglyceride control and decreasing lipid peroxidation, but with no effects on ameliorating blood glucose levels. The association of intervention protocols presented an additive effect on the antioxidant SOD activity in the muscle cells of diabetic rats.

Authors+Show Affiliations

Laboratory of Genetic Toxicology - UFCSPA, Brazil.Laboratory of Genetic Toxicology - UFCSPA, Brazil.Laboratory of Genetic Toxicology - UFCSPA, Brazil.Laboratory of Genetic Toxicology - UFCSPA, Brazil.Laboratory of Genetic Toxicology - UFCSPA, Brazil.Laboratory of Genetic Toxicology - UFCSPA, Brazil.Laboratory of Physicochemical and Natural Products - Federal University of Pampa, Brazil.Laboratory of Physicochemical and Natural Products - Federal University of Pampa, Brazil.Laboratory of Experimental Physiology - UFCSPA, Brazil.Laboratory of Experimental Physiology - UFCSPA, Brazil.Laboratory of Genetic Toxicology - UFCSPA, Brazil. Electronic address: jenifers@ufcspa.edu.br.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27794509

Citation

Baldissera, G, et al. "Effects of Crude Hydroalcoholic Extract of Syzygium Cumini (L.) Skeels Leaves and Continuous Aerobic Training in Rats With Diabetes Induced By a High-fat Diet and Low Doses of Streptozotocin." Journal of Ethnopharmacology, vol. 194, 2016, pp. 1012-1021.
Baldissera G, Sperotto ND, Rosa HT, et al. Effects of crude hydroalcoholic extract of Syzygium cumini (L.) Skeels leaves and continuous aerobic training in rats with diabetes induced by a high-fat diet and low doses of streptozotocin. J Ethnopharmacol. 2016;194:1012-1021.
Baldissera, G., Sperotto, N. D., Rosa, H. T., Henn, J. G., Peres, V. F., Moura, D. J., Roehrs, R., Denardin, E. L., Dal Lago, P., Nunes, R. B., & Saffi, J. (2016). Effects of crude hydroalcoholic extract of Syzygium cumini (L.) Skeels leaves and continuous aerobic training in rats with diabetes induced by a high-fat diet and low doses of streptozotocin. Journal of Ethnopharmacology, 194, 1012-1021. https://doi.org/10.1016/j.jep.2016.10.056
Baldissera G, et al. Effects of Crude Hydroalcoholic Extract of Syzygium Cumini (L.) Skeels Leaves and Continuous Aerobic Training in Rats With Diabetes Induced By a High-fat Diet and Low Doses of Streptozotocin. J Ethnopharmacol. 2016 Dec 24;194:1012-1021. PubMed PMID: 27794509.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of crude hydroalcoholic extract of Syzygium cumini (L.) Skeels leaves and continuous aerobic training in rats with diabetes induced by a high-fat diet and low doses of streptozotocin. AU - Baldissera,G, AU - Sperotto,N D M, AU - Rosa,H T, AU - Henn,J G, AU - Peres,V F, AU - Moura,D J, AU - Roehrs,R, AU - Denardin,E L G, AU - Dal Lago,P, AU - Nunes,R B, AU - Saffi,J, Y1 - 2016/10/27/ PY - 2016/05/25/received PY - 2016/10/11/revised PY - 2016/10/19/accepted PY - 2016/10/31/pubmed PY - 2017/4/12/medline PY - 2016/10/31/entrez KW - DNA damage KW - Exercise KW - Experimental diabetes mellitus KW - Oxidative stress KW - Syzygium cumini SP - 1012 EP - 1021 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 194 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: The leaves of Syzygium cumini (L.) or Skeels (Myrtaceae) are widely used in Brazilian folk medicine to treat diabetes. AIM OF THE STUDY: The present study evaluated the functional capacity, biochemical parameters, oxidative stress and DNA damage from eight weeks of intervention with a crude hydroalcoholic extract of S. cumini leaves (EBH) and continuous aerobic training (TAC) in diabetic (D) rats. MATERIALS AND METHODS: A hydroalcoholic (50%) extract was prepared by ultrasound and phytochemical parameters (total phenols, total tannins and myricetin content) were analyzed. Thirty-seven male Wistar rats were divided into five groups: normoglycemic controls (CONT), diabetic controls (D-CONT), diabetics treated with extract (D+EBH), trained diabetic (D+TAC) and diabetics treated with extract and trained (D+EBH+TAC). Functional capacity was assessed with a maximum exercise capacity test; biochemical parameters with enzymatic kits; oxidative stress by superoxide dismutase (SOD), catalase (CAT), thiobarbituric acid reactive substances (TBARS) and oxidized dichlorofluorescein (DCF), and the DNA damage by the comet assay. RESULTS: The D+TAC and D+EBH+TAC groups showed better functional capacity at the end of interventions. The D+EBH group showed glucose and triglyceride reduction, lowest DNA damage index in the blood, liver, kidney, heart, lung and gastrocnemius muscle, improved SOD levels in the liver, kidney and lung, improved CAT levels in the kidney and lower lipid peroxidation in all tissues studied, compared to the D-CONT group. The exercise (D+TAC) was effective in reducing triglycerides, improving SOD levels in the lung, reducing lipid peroxidation in all tissues studied and reducing the DCF oxidation in the kidney, in addition to protecting against DNA damage in the blood and heart. However, the additive effect of the intervention protocols when combined (EBH+TAC) was observed only in improving the gastrocnemius SOD levels. The phytochemical analyses showed a high content of phenols and the presence of myricetin glycosides. CONCLUSION: The findings in this study suggest a crude hydroalcoholic extract of S. cumini leaves has potential hypoglycemic, hypolipidemic and protective properties acting against oxidative stress and against DNA damage, probably due to its phenols and myricetin glycoside content and the antioxidant properties of these constituents. Moreover, exercise was suggested to have beneficial effects on diabetes, improving functional capacity, ameliorating blood triglyceride control and decreasing lipid peroxidation, but with no effects on ameliorating blood glucose levels. The association of intervention protocols presented an additive effect on the antioxidant SOD activity in the muscle cells of diabetic rats. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/27794509/Effects_of_crude_hydroalcoholic_extract_of_Syzygium_cumini__L___Skeels_leaves_and_continuous_aerobic_training_in_rats_with_diabetes_induced_by_a_high_fat_diet_and_low_doses_of_streptozotocin_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(16)31437-4 DB - PRIME DP - Unbound Medicine ER -