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Inhibition of phosphatidylinositol 3,5-bisphosphate production has pleiotropic effects on various membrane trafficking routes in Arabidopsis.
Plant Cell Physiol. 2017 01 01; 58(1):120-129.PC

Abstract

Phosphoinositides play an important role in various membrane trafficking events in eukaryotes. One of them, however, phosphatidylinositol 3,5-bisphosphate [PI(3,5)P2], has not been studied widely in plants. Using a combination of fluorescent reporter proteins and the PI(3,5)P2-specific inhibitor YM202636, here we demonstrated that in Arabidopsis thaliana, PI(3,5)P2 affects various membrane trafficking events, mostly in the post-Golgi routes. We found that YM201636 treatment effectively reduced PI(3,5)P2 concentration not only in the wild type but also in FAB1A-overexpressing Arabidopsis plants. In particular, reduced PI(3,5)P2 levels caused abnormal membrane dynamics of plasma membrane proteins, AUX1 and BOR1, with different trafficking patterns. Secretion and morphological characteristics of late endosomes and vacuoles were also affected by the decreased PI(3,5)P2 production. These pleiotropic defects in the post-Golgi trafficking events were caused by the inhibition of PI(3,5)P2 production. This effect is probably mediated by the inhibition of maturation of FAB1-positive late endosomes, thereby impairing late endosome function. In conclusion, our results imply that in Arabidopsis, late endosomes are involved in multiple post-Golgi membrane trafficking routes including not only vacuolar trafficking and endocytosis but also secretion.

Authors+Show Affiliations

Laboratory of Cellular Dynamics, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Kyoto, Japan.Plant Physiology, Swammerdam Institute for Life Sciences, University of Amsterdam, Amsterdam, The Netherlands.Laboratory of Cellular Dynamics, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University, Kyoto, Japan.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27803131

Citation

Hirano, Tomoko, et al. "Inhibition of Phosphatidylinositol 3,5-bisphosphate Production Has Pleiotropic Effects On Various Membrane Trafficking Routes in Arabidopsis." Plant & Cell Physiology, vol. 58, no. 1, 2017, pp. 120-129.
Hirano T, Munnik T, Sato MH. Inhibition of phosphatidylinositol 3,5-bisphosphate production has pleiotropic effects on various membrane trafficking routes in Arabidopsis. Plant Cell Physiol. 2017;58(1):120-129.
Hirano, T., Munnik, T., & Sato, M. H. (2017). Inhibition of phosphatidylinositol 3,5-bisphosphate production has pleiotropic effects on various membrane trafficking routes in Arabidopsis. Plant & Cell Physiology, 58(1), 120-129. https://doi.org/10.1093/pcp/pcw164
Hirano T, Munnik T, Sato MH. Inhibition of Phosphatidylinositol 3,5-bisphosphate Production Has Pleiotropic Effects On Various Membrane Trafficking Routes in Arabidopsis. Plant Cell Physiol. 2017 01 1;58(1):120-129. PubMed PMID: 27803131.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibition of phosphatidylinositol 3,5-bisphosphate production has pleiotropic effects on various membrane trafficking routes in Arabidopsis. AU - Hirano,Tomoko, AU - Munnik,Teun, AU - Sato,Masa H, PY - 2016/05/04/received PY - 2016/09/15/accepted PY - 2016/11/3/pubmed PY - 2017/7/19/medline PY - 2016/11/3/entrez KW - Arabidopsis thaliana KW - FAB1/PIKfyve KW - Phosphatidylinositol 3,5-bisphosphate KW - Post-Golgi membrane trafficking routes KW - YM201636 SP - 120 EP - 129 JF - Plant & cell physiology JO - Plant Cell Physiol. VL - 58 IS - 1 N2 - Phosphoinositides play an important role in various membrane trafficking events in eukaryotes. One of them, however, phosphatidylinositol 3,5-bisphosphate [PI(3,5)P2], has not been studied widely in plants. Using a combination of fluorescent reporter proteins and the PI(3,5)P2-specific inhibitor YM202636, here we demonstrated that in Arabidopsis thaliana, PI(3,5)P2 affects various membrane trafficking events, mostly in the post-Golgi routes. We found that YM201636 treatment effectively reduced PI(3,5)P2 concentration not only in the wild type but also in FAB1A-overexpressing Arabidopsis plants. In particular, reduced PI(3,5)P2 levels caused abnormal membrane dynamics of plasma membrane proteins, AUX1 and BOR1, with different trafficking patterns. Secretion and morphological characteristics of late endosomes and vacuoles were also affected by the decreased PI(3,5)P2 production. These pleiotropic defects in the post-Golgi trafficking events were caused by the inhibition of PI(3,5)P2 production. This effect is probably mediated by the inhibition of maturation of FAB1-positive late endosomes, thereby impairing late endosome function. In conclusion, our results imply that in Arabidopsis, late endosomes are involved in multiple post-Golgi membrane trafficking routes including not only vacuolar trafficking and endocytosis but also secretion. SN - 1471-9053 UR - https://www.unboundmedicine.com/medline/citation/27803131/Inhibition_of_phosphatidylinositol_35_bisphosphate_production_has_pleiotropic_effects_on_various_membrane_trafficking_routes_in_Arabidopsis_ L2 - https://academic.oup.com/pcp/article-lookup/doi/10.1093/pcp/pcw164 DB - PRIME DP - Unbound Medicine ER -