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The Chemical Profile of Senna velutina Leaves and Their Antioxidant and Cytotoxic Effects.
Oxid Med Cell Longev. 2016; 2016:8405957.OM

Abstract

Natural products can be a source of biomolecules with antioxidant activity which are able to prevent oxidative stress-induced diseases and show antitumor activity, making them important sources of new anticancer drug prototypes. In this context, this study aimed to analyze the chemical composition of an ethanol extract of Senna velutina leaves and to assess its antioxidant and cytotoxic activities in leukemic cells. The antioxidant properties were evaluated using a DPPH free radical scavenging assay and by examining the extract's inhibition of AAPH-induced lipid peroxidation in human erythrocytes. Its cytotoxicity and possible mechanisms of action were assessed in Jurkat and K562 leukemic cell lines. The ethanol extract contained flavonoids, such as epigallocatechin, epicatechin, kaempferol heteroside, rutin, and dimeric and trimeric proanthocyanidin derivatives. The extract exhibited antioxidant activity by scavenging free radicals and antihemolytic action, and it decreased malondialdehyde content in human erythrocytes. Furthermore, the extract also induced leukemic cell death by activating intracellular calcium and caspase-3, decreasing mitochondrial membrane potential, and arresting the cell cycle in S and G2 phases. Hence, S. velutina leaf extract contains antioxidant and antileukemic biomolecules with potential applications in diseases associated with oxidative stress and in the inhibition of tumor cell proliferation.

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Authors+Show Affiliations

Campos JF
School of Environmental and Biological Science, Federal University of Grande Dourados, Rodovia Dourados Itahum, Km 12, 79.804-970 Dourados, MS, Brazil.
de Castro DT
School of Environmental and Biological Science, Federal University of Grande Dourados, Rodovia Dourados Itahum, Km 12, 79.804-970 Dourados, MS, Brazil.
Damião MJ
School of Environmental and Biological Science, Federal University of Grande Dourados, Rodovia Dourados Itahum, Km 12, 79.804-970 Dourados, MS, Brazil.
Vieira Torquato HF
Department of Biochemistry, Federal University of São Paulo, Rua Pedro de Toledo 669, 04039-032 São Paulo, SP, Brazil.
Paredes-Gamero EJ
Department of Biochemistry, Federal University of São Paulo, Rua Pedro de Toledo 669, 04039-032 São Paulo, SP, Brazil; Interdisciplinary Center of Biochemistry Investigation, University of Mogi das Cruzes, Av. Dr. Cândido Xavier de Almeida Souza, No. 200, Mogi das Cruzes, SP, Brazil.
Carollo CA
Center of Biological and Health Sciences, Federal University of Mato Grosso do Sul, Campo Grande, MS, Brazil.
Estevinho LM
Department of Biology and Biotechnology, Agricultural College of Bragança, Polytechnic Institute of Bragança, Campus Santa Apolónia, 5301-855 Bragança, Portugal.
de Picoli Souza K
School of Environmental and Biological Science, Federal University of Grande Dourados, Rodovia Dourados Itahum, Km 12, 79.804-970 Dourados, MS, Brazil.
Dos Santos EL
School of Environmental and Biological Science, Federal University of Grande Dourados, Rodovia Dourados Itahum, Km 12, 79.804-970 Dourados, MS, Brazil.

MeSH

Antineoplastic Agents, PhytogenicAntioxidantsBiphenyl CompoundsCalciumCaspase 3Cell ProliferationCell SurvivalDose-Response Relationship, DrugErythrocytesG2 Phase Cell Cycle CheckpointsHumansInhibitory Concentration 50Jurkat CellsK562 CellsLeukemiaLipid PeroxidationMalondialdehydeMembrane Potential, MitochondrialOxidative StressPhytochemicalsPhytotherapyPicratesPlant ExtractsPlant LeavesPlants, MedicinalS Phase Cell Cycle CheckpointsSenna Plant

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27803764

Citation

Campos, Jaqueline Ferreira, et al. "The Chemical Profile of Senna Velutina Leaves and Their Antioxidant and Cytotoxic Effects." Oxidative Medicine and Cellular Longevity, vol. 2016, 2016, p. 8405957.
Campos JF, de Castro DT, Damião MJ, et al. The Chemical Profile of Senna velutina Leaves and Their Antioxidant and Cytotoxic Effects. Oxid Med Cell Longev. 2016;2016:8405957.
Campos, J. F., de Castro, D. T., Damião, M. J., Vieira Torquato, H. F., Paredes-Gamero, E. J., Carollo, C. A., Estevinho, L. M., de Picoli Souza, K., & Dos Santos, E. L. (2016). The Chemical Profile of Senna velutina Leaves and Their Antioxidant and Cytotoxic Effects. Oxidative Medicine and Cellular Longevity, 2016, 8405957.
Campos JF, et al. The Chemical Profile of Senna Velutina Leaves and Their Antioxidant and Cytotoxic Effects. Oxid Med Cell Longev. 2016;2016:8405957. PubMed PMID: 27803764.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The Chemical Profile of Senna velutina Leaves and Their Antioxidant and Cytotoxic Effects. AU - Campos,Jaqueline Ferreira, AU - de Castro,David Tsuyoshi Hiramatsu, AU - Damião,Marcio José, AU - Vieira Torquato,Heron F, AU - Paredes-Gamero,Edgar J, AU - Carollo,Carlos Alexandre, AU - Estevinho,Leticia M, AU - de Picoli Souza,Kely, AU - Dos Santos,Edson Lucas, Y1 - 2016/10/10/ PY - 2016/06/04/received PY - 2016/09/14/accepted PY - 2016/11/3/pubmed PY - 2017/3/14/medline PY - 2016/11/3/entrez SP - 8405957 EP - 8405957 JF - Oxidative medicine and cellular longevity JO - Oxid Med Cell Longev VL - 2016 N2 - Natural products can be a source of biomolecules with antioxidant activity which are able to prevent oxidative stress-induced diseases and show antitumor activity, making them important sources of new anticancer drug prototypes. In this context, this study aimed to analyze the chemical composition of an ethanol extract of Senna velutina leaves and to assess its antioxidant and cytotoxic activities in leukemic cells. The antioxidant properties were evaluated using a DPPH free radical scavenging assay and by examining the extract's inhibition of AAPH-induced lipid peroxidation in human erythrocytes. Its cytotoxicity and possible mechanisms of action were assessed in Jurkat and K562 leukemic cell lines. The ethanol extract contained flavonoids, such as epigallocatechin, epicatechin, kaempferol heteroside, rutin, and dimeric and trimeric proanthocyanidin derivatives. The extract exhibited antioxidant activity by scavenging free radicals and antihemolytic action, and it decreased malondialdehyde content in human erythrocytes. Furthermore, the extract also induced leukemic cell death by activating intracellular calcium and caspase-3, decreasing mitochondrial membrane potential, and arresting the cell cycle in S and G2 phases. Hence, S. velutina leaf extract contains antioxidant and antileukemic biomolecules with potential applications in diseases associated with oxidative stress and in the inhibition of tumor cell proliferation. SN - 1942-0994 UR - https://www.unboundmedicine.com/medline/citation/27803764/The_Chemical_Profile_of_Senna_velutina_Leaves_and_Their_Antioxidant_and_Cytotoxic_Effects_ DB - PRIME DP - Unbound Medicine ER -