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Phenolic Profile and Antioxidant Activity of Centaurea choulettiana Pomel (Asteraceae) Extracts.
Comb Chem High Throughput Screen 2016; 19(10):841-846CC

Abstract

AIM AND OBJECTIVE

This study aimed to quantify phenolic compounds in ethyl acetate and n-butanol extract of Centaurea choulettiana Pomel (Asteraceae) leaves and flowers; compare the antioxidant activity of their extracts, identification and quantification of their phenolic acids.

MATERIALS AND METHODS

Both organs extracts of Centaurea choulettiana Pomel were investigated and evaluated for their potential antioxidant properties using total phenolics and flavonoids content, DPPH radical scavenging and lipid peroxidation inhibition assays. HPLC-TOF/MS analyses were carried out to identify and quantify some phenolic acids.

RESULTS

The amounts of phenolic and flavonoid content were higher in ethyl acetate extract of leaves (325.81 ± 0.038 mgGAE and 263.73 ± 0.004 mgQE /g of extract) respectively. Besides, this extract exhibited the most powerful effect on the DPPH radical scavenging activity with (96.54%), on lipid peroxydation inhibition (64.17%). Ethyl acetate extract of leaves and flowers were found to contain almost the same phenolic compounds, with the leaves having the highest values. Chlorogenic acid was detected in the n-butanol extract of flowers with the highest concentration 17.78 mg/kg plant.

CONCLUSION

The ethyl acetate extract of leaves of Centaurea choulettiana possesses strong antioxidative properties in vitro. They are confirmed by high polyphenols and flavonoids content. The HPLC-TOF/MS analysis reveals the presence of 4-hydroxybenzoic acid, gentisic acid, chlorogenic acid, caffeic acid, vanillic acid, p-Coumaric acid, ferulic acid, salicylic acid and protocatechuic acid.

Authors+Show Affiliations

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableUnite de Recherche Valorisation des Ressources Naturelles, Molecules Bioactives et Analyses Physicochimiques et Biologiques (VARENBIOMOL), Departement de Chimie, Faculte des Sciences Exactes, Universite Constantine 1, 25000 Constantine, Algerie.. Algeria.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27809745

Citation

Azzouzi, Djihane, et al. "Phenolic Profile and Antioxidant Activity of Centaurea Choulettiana Pomel (Asteraceae) Extracts." Combinatorial Chemistry & High Throughput Screening, vol. 19, no. 10, 2016, pp. 841-846.
Azzouzi D, Bioud K, Demirtas I, et al. Phenolic Profile and Antioxidant Activity of Centaurea choulettiana Pomel (Asteraceae) Extracts. Comb Chem High Throughput Screen. 2016;19(10):841-846.
Azzouzi, D., Bioud, K., Demirtas, I., Gul, F., Sarri, D., Benayache, S., ... Mekkiou, R. (2016). Phenolic Profile and Antioxidant Activity of Centaurea choulettiana Pomel (Asteraceae) Extracts. Combinatorial Chemistry & High Throughput Screening, 19(10), pp. 841-846. doi:10.2174/1574888X11666161102092319.
Azzouzi D, et al. Phenolic Profile and Antioxidant Activity of Centaurea Choulettiana Pomel (Asteraceae) Extracts. Comb Chem High Throughput Screen. 2016;19(10):841-846. PubMed PMID: 27809745.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Phenolic Profile and Antioxidant Activity of Centaurea choulettiana Pomel (Asteraceae) Extracts. AU - Azzouzi,Djihane, AU - Bioud,Kenza, AU - Demirtas,Ibrahim, AU - Gul,Fatih, AU - Sarri,Djamel, AU - Benayache,Samir, AU - Benayache,Fadila, AU - Mekkiou,Ratiba, PY - 2016/06/18/received PY - 2016/10/07/revised PY - 2016/10/13/accepted PY - 2016/11/5/pubmed PY - 2017/6/24/medline PY - 2016/11/5/entrez KW - Centaurea choulettiana KW - HPLC-TOF/MS KW - antioxidant activity KW - phenolic acids KW - total flavonoids. KW - total phenolics SP - 841 EP - 846 JF - Combinatorial chemistry & high throughput screening JO - Comb. Chem. High Throughput Screen. VL - 19 IS - 10 N2 - AIM AND OBJECTIVE: This study aimed to quantify phenolic compounds in ethyl acetate and n-butanol extract of Centaurea choulettiana Pomel (Asteraceae) leaves and flowers; compare the antioxidant activity of their extracts, identification and quantification of their phenolic acids. MATERIALS AND METHODS: Both organs extracts of Centaurea choulettiana Pomel were investigated and evaluated for their potential antioxidant properties using total phenolics and flavonoids content, DPPH radical scavenging and lipid peroxidation inhibition assays. HPLC-TOF/MS analyses were carried out to identify and quantify some phenolic acids. RESULTS: The amounts of phenolic and flavonoid content were higher in ethyl acetate extract of leaves (325.81 ± 0.038 mgGAE and 263.73 ± 0.004 mgQE /g of extract) respectively. Besides, this extract exhibited the most powerful effect on the DPPH radical scavenging activity with (96.54%), on lipid peroxydation inhibition (64.17%). Ethyl acetate extract of leaves and flowers were found to contain almost the same phenolic compounds, with the leaves having the highest values. Chlorogenic acid was detected in the n-butanol extract of flowers with the highest concentration 17.78 mg/kg plant. CONCLUSION: The ethyl acetate extract of leaves of Centaurea choulettiana possesses strong antioxidative properties in vitro. They are confirmed by high polyphenols and flavonoids content. The HPLC-TOF/MS analysis reveals the presence of 4-hydroxybenzoic acid, gentisic acid, chlorogenic acid, caffeic acid, vanillic acid, p-Coumaric acid, ferulic acid, salicylic acid and protocatechuic acid. SN - 1875-5402 UR - https://www.unboundmedicine.com/medline/citation/27809745/Phenolic_Profile_and_Antioxidant_Activity_of_Centaurea_choulettiana_Pomel__Asteraceae__Extracts_ L2 - http://www.eurekaselect.com/146940/article DB - PRIME DP - Unbound Medicine ER -