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The ratio of Zn to Cd supply as a determinant of metal-homeostasis gene expression in tobacco and its modulation by overexpressing the metal exporter AtHMA4.
J Exp Bot. 2016 11; 67(21):6201-6214.JE

Abstract

This study links changes in the tobacco endogenous metal-homeostasis network caused by transgene expression with engineering of novel features. It also provides insight into the concentration-dependent mutual interactions between Zn and Cd, leading to differences in the metal partitioning between wild-type and transgenic plants. In tobacco, expression of the export protein AtHMA4 modified Zn/Cd root/shoot distribution, but the pattern depended on their concentrations in the medium. To address this phenomenon, the expression of genes identified by suppression subtractive hybridization and the Zn/Cd accumulation pattern were examined upon exposure to six variants of low/high Zn and Cd concentrations. Five tobacco metal-homeostasis genes were identified: NtZIP2, NtZIP4, NtIRT1-like, NtNAS, and NtVTL. In the wild type, their expression depended on combinations of low/high Zn and Cd concentrations; co-ordinated responses of NtZIP1, NtZIP2, and NtVTL were shown in medium containing 4 µM Cd, and at 0.5 µM versus 10 µM Zn. In transgenics, qualitative changes detected for NtZIP1, NtZIP4, NtIRT1-like, and NtVTL are considered crucial for modification of Zn/Cd supply-dependent Zn/Cd root/shoot distribution. Notwithstanding, NtVTL was the most responsive gene in wild-type and transgenic plants under all concentrations of Zn and Cd tested; thus it is a candidate gene for the regulation of metal cross-homeostasis processes involved in engineering new metal-related traits.

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Authors+Show Affiliations

Barabasz A
University of Warsaw, Faculty of Biology, Institute of Experimental Plant Biology and Biotechnology, Department of Plant Anatomy and Cytology, Miecznikowa str 1, 02-096 Warszawa, Poland.
Klimecka M
University of Warsaw, Faculty of Biology, Institute of Experimental Plant Biology and Biotechnology, Department of Plant Anatomy and Cytology, Miecznikowa str 1, 02-096 Warszawa, Poland.
Kendziorek M
University of Warsaw, Faculty of Biology, Institute of Experimental Plant Biology and Biotechnology, Department of Plant Anatomy and Cytology, Miecznikowa str 1, 02-096 Warszawa, Poland.
Weremczuk A
University of Warsaw, Faculty of Biology, Institute of Experimental Plant Biology and Biotechnology, Department of Plant Anatomy and Cytology, Miecznikowa str 1, 02-096 Warszawa, Poland.
Ruszczyńska A
University of Warsaw, Faculty of Chemistry, Pasteura str. 1, 02-093 Warszawa, Poland.
Bulska E
University of Warsaw, Faculty of Chemistry, Pasteura str. 1, 02-093 Warszawa, Poland.
Antosiewicz DM
University of Warsaw, Faculty of Biology, Institute of Experimental Plant Biology and Biotechnology, Department of Plant Anatomy and Cytology, Miecznikowa str 1, 02-096 Warszawa, Poland dma@biol.uw.edu.pl.

MeSH

Adenosine TriphosphatasesArabidopsis ProteinsCadmiumCation Transport ProteinsGene Expression ProfilingGene Expression Regulation, PlantHomeostasisPlant ProteinsPlants, Genetically ModifiedReal-Time Polymerase Chain ReactionSequence Analysis, DNASubtractive Hybridization TechniquesTobaccoZinc

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27811086

Citation

Barabasz, Anna, et al. "The Ratio of Zn to Cd Supply as a Determinant of Metal-homeostasis Gene Expression in Tobacco and Its Modulation By Overexpressing the Metal Exporter AtHMA4." Journal of Experimental Botany, vol. 67, no. 21, 2016, pp. 6201-6214.
Barabasz A, Klimecka M, Kendziorek M, et al. The ratio of Zn to Cd supply as a determinant of metal-homeostasis gene expression in tobacco and its modulation by overexpressing the metal exporter AtHMA4. J Exp Bot. 2016;67(21):6201-6214.
Barabasz, A., Klimecka, M., Kendziorek, M., Weremczuk, A., Ruszczyńska, A., Bulska, E., & Antosiewicz, D. M. (2016). The ratio of Zn to Cd supply as a determinant of metal-homeostasis gene expression in tobacco and its modulation by overexpressing the metal exporter AtHMA4. Journal of Experimental Botany, 67(21), 6201-6214.
Barabasz A, et al. The Ratio of Zn to Cd Supply as a Determinant of Metal-homeostasis Gene Expression in Tobacco and Its Modulation By Overexpressing the Metal Exporter AtHMA4. J Exp Bot. 2016;67(21):6201-6214. PubMed PMID: 27811086.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The ratio of Zn to Cd supply as a determinant of metal-homeostasis gene expression in tobacco and its modulation by overexpressing the metal exporter AtHMA4. AU - Barabasz,Anna, AU - Klimecka,Maria, AU - Kendziorek,Maria, AU - Weremczuk,Aleksandra, AU - Ruszczyńska,Anna, AU - Bulska,Ewa, AU - Antosiewicz,Danuta Maria, Y1 - 2016/10/17/ PY - 2016/11/5/pubmed PY - 2017/11/14/medline PY - 2016/11/5/entrez KW - AtHMA4 KW - SSH KW - cadmium KW - tobacco KW - transformation KW - zinc. SP - 6201 EP - 6214 JF - Journal of experimental botany JO - J Exp Bot VL - 67 IS - 21 N2 - This study links changes in the tobacco endogenous metal-homeostasis network caused by transgene expression with engineering of novel features. It also provides insight into the concentration-dependent mutual interactions between Zn and Cd, leading to differences in the metal partitioning between wild-type and transgenic plants. In tobacco, expression of the export protein AtHMA4 modified Zn/Cd root/shoot distribution, but the pattern depended on their concentrations in the medium. To address this phenomenon, the expression of genes identified by suppression subtractive hybridization and the Zn/Cd accumulation pattern were examined upon exposure to six variants of low/high Zn and Cd concentrations. Five tobacco metal-homeostasis genes were identified: NtZIP2, NtZIP4, NtIRT1-like, NtNAS, and NtVTL. In the wild type, their expression depended on combinations of low/high Zn and Cd concentrations; co-ordinated responses of NtZIP1, NtZIP2, and NtVTL were shown in medium containing 4 µM Cd, and at 0.5 µM versus 10 µM Zn. In transgenics, qualitative changes detected for NtZIP1, NtZIP4, NtIRT1-like, and NtVTL are considered crucial for modification of Zn/Cd supply-dependent Zn/Cd root/shoot distribution. Notwithstanding, NtVTL was the most responsive gene in wild-type and transgenic plants under all concentrations of Zn and Cd tested; thus it is a candidate gene for the regulation of metal cross-homeostasis processes involved in engineering new metal-related traits. SN - 1460-2431 UR - https://www.unboundmedicine.com/medline/citation/27811086/The_ratio_of_Zn_to_Cd_supply_as_a_determinant_of_metal_homeostasis_gene_expression_in_tobacco_and_its_modulation_by_overexpressing_the_metal_exporter_AtHMA4_ DB - PRIME DP - Unbound Medicine ER -