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The prevalence and characterization of antibiotic-resistant and virulent Escherichia coli strains in the municipal wastewater system and their environmental fate.
Sci Total Environ. 2017 Jan 15; 577:367-375.ST

Abstract

Antibiotics are widely used in human and veterinary medicine and in animal production, which increases their concentrations in aquatic ecosystems and contributes to selective pressure on environmental microorganisms. The objective of this study was to identify antibiotic resistance determinants in Escherichia coli strains isolated from untreated and treated wastewater (UWW and TWW) and from river water sampled downstream and upstream (URW and DRW) from the effluent discharge point. The analyzed antibiotic groups were beta-lactams, tetracyclines and fluoroquinolones which are widely used in human and veterinary medicine. The virulence of the isolated E. coli strains was also analyzed, and their clonal relatedness was determined by ERIC (enterobacterial repetitive intergenic consensus sequence) PCR. The highest counts of bacteria resistant to beta-lactams, tetracyclines and fluoroquinolones were noted in UWW at 6.4×104, 4.2×104 and 3.1×103CFU/mL, respectively. A total of 317 E. coli isolates resistant to at least one group of antibiotics were selected among bacterial isolates from river water and wastewater samples. Nearly 38% of those isolates were resistant to all of the tested antibiotics. The highest percent (43%) of multidrug-resistant isolates was noted in UWW samples. Isolates resistant to beta-lactams most frequently harbored blaTEM and blaOXA genes. The group of genes encoding resistance to tetracyclines was most frequently represented by tetA, tetB and tetK, whereas the qnrS gene was noted in isolates resistant to fluoroquinolones. Virulence genes bfpA (65%), ST (56%) and eae (39%) were most widely distributed in all isolates, regardless of their origin. The results of this experiment reveal the dangers associated with environmental contamination by drug-resistant and virulent E. coli strains distributed with treated wastewater. Multidrug resistance was determined more frequently in strains isolated from DRW than in isolates from URW samples. Our findings provide valuable inputs for evaluating public health hazards associated with bacterial contamination.

Authors+Show Affiliations

Department of Environmental Microbiology, Faculty of Environmental Sciences, University of Warmia and Mazury in Olsztyn, Prawocheńskiego 1 Str., 10-720 Olsztyn, Poland. Electronic address: adriana.osinska@uwm.edu.pl.Department of Environmental Microbiology, Faculty of Environmental Sciences, University of Warmia and Mazury in Olsztyn, Prawocheńskiego 1 Str., 10-720 Olsztyn, Poland. Electronic address: ewa.korzeniewska@uwm.edu.pl.Department of Environmental Microbiology, Faculty of Environmental Sciences, University of Warmia and Mazury in Olsztyn, Prawocheńskiego 1 Str., 10-720 Olsztyn, Poland. Electronic address: monika.harnisz@uwm.edu.pl.Department of Environmental Microbiology, Faculty of Environmental Sciences, University of Warmia and Mazury in Olsztyn, Prawocheńskiego 1 Str., 10-720 Olsztyn, Poland. Electronic address: niestepski.sebastian@gmail.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27816226

Citation

Osińska, Adriana, et al. "The Prevalence and Characterization of Antibiotic-resistant and Virulent Escherichia Coli Strains in the Municipal Wastewater System and Their Environmental Fate." The Science of the Total Environment, vol. 577, 2017, pp. 367-375.
Osińska A, Korzeniewska E, Harnisz M, et al. The prevalence and characterization of antibiotic-resistant and virulent Escherichia coli strains in the municipal wastewater system and their environmental fate. Sci Total Environ. 2017;577:367-375.
Osińska, A., Korzeniewska, E., Harnisz, M., & Niestępski, S. (2017). The prevalence and characterization of antibiotic-resistant and virulent Escherichia coli strains in the municipal wastewater system and their environmental fate. The Science of the Total Environment, 577, 367-375. https://doi.org/10.1016/j.scitotenv.2016.10.203
Osińska A, et al. The Prevalence and Characterization of Antibiotic-resistant and Virulent Escherichia Coli Strains in the Municipal Wastewater System and Their Environmental Fate. Sci Total Environ. 2017 Jan 15;577:367-375. PubMed PMID: 27816226.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The prevalence and characterization of antibiotic-resistant and virulent Escherichia coli strains in the municipal wastewater system and their environmental fate. AU - Osińska,Adriana, AU - Korzeniewska,Ewa, AU - Harnisz,Monika, AU - Niestępski,Sebastian, Y1 - 2016/11/03/ PY - 2016/09/13/received PY - 2016/10/26/revised PY - 2016/10/26/accepted PY - 2016/11/7/pubmed PY - 2018/6/7/medline PY - 2016/11/7/entrez KW - Antibiotics KW - Escherichia coli KW - Multidrug resistance KW - Surface water KW - Virulence KW - Wastewater SP - 367 EP - 375 JF - The Science of the total environment JO - Sci. Total Environ. VL - 577 N2 - Antibiotics are widely used in human and veterinary medicine and in animal production, which increases their concentrations in aquatic ecosystems and contributes to selective pressure on environmental microorganisms. The objective of this study was to identify antibiotic resistance determinants in Escherichia coli strains isolated from untreated and treated wastewater (UWW and TWW) and from river water sampled downstream and upstream (URW and DRW) from the effluent discharge point. The analyzed antibiotic groups were beta-lactams, tetracyclines and fluoroquinolones which are widely used in human and veterinary medicine. The virulence of the isolated E. coli strains was also analyzed, and their clonal relatedness was determined by ERIC (enterobacterial repetitive intergenic consensus sequence) PCR. The highest counts of bacteria resistant to beta-lactams, tetracyclines and fluoroquinolones were noted in UWW at 6.4×104, 4.2×104 and 3.1×103CFU/mL, respectively. A total of 317 E. coli isolates resistant to at least one group of antibiotics were selected among bacterial isolates from river water and wastewater samples. Nearly 38% of those isolates were resistant to all of the tested antibiotics. The highest percent (43%) of multidrug-resistant isolates was noted in UWW samples. Isolates resistant to beta-lactams most frequently harbored blaTEM and blaOXA genes. The group of genes encoding resistance to tetracyclines was most frequently represented by tetA, tetB and tetK, whereas the qnrS gene was noted in isolates resistant to fluoroquinolones. Virulence genes bfpA (65%), ST (56%) and eae (39%) were most widely distributed in all isolates, regardless of their origin. The results of this experiment reveal the dangers associated with environmental contamination by drug-resistant and virulent E. coli strains distributed with treated wastewater. Multidrug resistance was determined more frequently in strains isolated from DRW than in isolates from URW samples. Our findings provide valuable inputs for evaluating public health hazards associated with bacterial contamination. SN - 1879-1026 UR - https://www.unboundmedicine.com/medline/citation/27816226/The_prevalence_and_characterization_of_antibiotic_resistant_and_virulent_Escherichia_coli_strains_in_the_municipal_wastewater_system_and_their_environmental_fate_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0048-9697(16)32394-4 DB - PRIME DP - Unbound Medicine ER -