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Clinical benefit of component-resolved diagnosis in Japanese birch-allergic patients with a convincing history of apple or peach allergy.
Auris Nasus Larynx. 2017 Aug; 44(4):442-446.AN

Abstract

OBJECTIVE

In northern Japan, birch pollen is the major allergen in pollinosis, while oral allergy syndrome (OAS) is caused primarily by apple and peach, and is almost exclusively related to birch pollinosis. To clarify the clinical benefit of allergen-based component-resolved diagnosis (CRD) in Japanese birch-allergic patients with OAS, we present an analysis of IgE profiles in response to crude extracts and recombinant component-resolved allergen to birch pollen and Rosaceae fruits allergens.

METHODS

The sera of 30 patients with birch pollen-related OAS to apple or peach were analyzed for specific IgE reactivity to pathogenesis-related class 10 (PR-10) family (birch: rBet v 1, apple: rMal d 1, and peach: rPru p 1), profilin (birch: rBet v 2 and peach: rPru p 4), and lipid transfer protein (LTP) (apple: rMal d 3 and peach: rPru p 3) allergens, as well as to conventional crude, unfractionated extracts (birch: T3, apple: f49, and peach: f95) using the ImmunoCAP System™. Allergen-specific IgE values <0.35kUA/L were considered negative.

RESULTS

Of the 30 sera CAP-positive for natural birch pollen extract, 28 (93.3%) exhibited specific IgE against Bet v1, and two (6.7%) contained specific IgE against Bet v2. Of the 26 sera of OAS to apple patients, only 17 were positive for specific IgE against f49 extract (65.4%); however, 24 were positive for specific IgE against rMal d 1 (92.3%). Similarly, only 17 of the 23 sera of OAS to peach patients contained specific IgE against the f95 extract (73.9%); however, 22 were positive for specific IgE against rPru p 1 (95.7%).

CONCLUSION

Our data suggest that CRD constitutes a reliable tool for the diagnosis of birch pollen-related OAS.

Authors+Show Affiliations

Department of Otolaryngology, Sapporo Medical University, School of Medicine, Japan. Electronic address: shira@sapmed.ac.jp.Yamamoto ENT Clinic, Japan.ThermoFisher Diagnostics Japan, Japan.Department of Otolaryngology, Sapporo Medical University, School of Medicine, Japan.Department of Otolaryngology, Sapporo Medical University, School of Medicine, Japan.Department of Otolaryngology, Sapporo Medical University, School of Medicine, Japan.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27816237

Citation

Shirasaki, Hideaki, et al. "Clinical Benefit of Component-resolved Diagnosis in Japanese Birch-allergic Patients With a Convincing History of Apple or Peach Allergy." Auris, Nasus, Larynx, vol. 44, no. 4, 2017, pp. 442-446.
Shirasaki H, Yamamoto T, Abe S, et al. Clinical benefit of component-resolved diagnosis in Japanese birch-allergic patients with a convincing history of apple or peach allergy. Auris Nasus Larynx. 2017;44(4):442-446.
Shirasaki, H., Yamamoto, T., Abe, S., Kanaizumi, E., Kikuchi, M., & Himi, T. (2017). Clinical benefit of component-resolved diagnosis in Japanese birch-allergic patients with a convincing history of apple or peach allergy. Auris, Nasus, Larynx, 44(4), 442-446. https://doi.org/10.1016/j.anl.2016.10.004
Shirasaki H, et al. Clinical Benefit of Component-resolved Diagnosis in Japanese Birch-allergic Patients With a Convincing History of Apple or Peach Allergy. Auris Nasus Larynx. 2017;44(4):442-446. PubMed PMID: 27816237.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Clinical benefit of component-resolved diagnosis in Japanese birch-allergic patients with a convincing history of apple or peach allergy. AU - Shirasaki,Hideaki, AU - Yamamoto,Tetsuo, AU - Abe,Shu, AU - Kanaizumi,Etsuko, AU - Kikuchi,Megumi, AU - Himi,Tetsuo, Y1 - 2016/11/03/ PY - 2016/06/16/received PY - 2016/08/31/revised PY - 2016/10/06/accepted PY - 2016/11/7/pubmed PY - 2018/3/22/medline PY - 2016/11/7/entrez KW - Birch pollinosis KW - Component-resolved diagnosis KW - Oral allergy syndrome SP - 442 EP - 446 JF - Auris, nasus, larynx JO - Auris Nasus Larynx VL - 44 IS - 4 N2 - OBJECTIVE: In northern Japan, birch pollen is the major allergen in pollinosis, while oral allergy syndrome (OAS) is caused primarily by apple and peach, and is almost exclusively related to birch pollinosis. To clarify the clinical benefit of allergen-based component-resolved diagnosis (CRD) in Japanese birch-allergic patients with OAS, we present an analysis of IgE profiles in response to crude extracts and recombinant component-resolved allergen to birch pollen and Rosaceae fruits allergens. METHODS: The sera of 30 patients with birch pollen-related OAS to apple or peach were analyzed for specific IgE reactivity to pathogenesis-related class 10 (PR-10) family (birch: rBet v 1, apple: rMal d 1, and peach: rPru p 1), profilin (birch: rBet v 2 and peach: rPru p 4), and lipid transfer protein (LTP) (apple: rMal d 3 and peach: rPru p 3) allergens, as well as to conventional crude, unfractionated extracts (birch: T3, apple: f49, and peach: f95) using the ImmunoCAP System™. Allergen-specific IgE values <0.35kUA/L were considered negative. RESULTS: Of the 30 sera CAP-positive for natural birch pollen extract, 28 (93.3%) exhibited specific IgE against Bet v1, and two (6.7%) contained specific IgE against Bet v2. Of the 26 sera of OAS to apple patients, only 17 were positive for specific IgE against f49 extract (65.4%); however, 24 were positive for specific IgE against rMal d 1 (92.3%). Similarly, only 17 of the 23 sera of OAS to peach patients contained specific IgE against the f95 extract (73.9%); however, 22 were positive for specific IgE against rPru p 1 (95.7%). CONCLUSION: Our data suggest that CRD constitutes a reliable tool for the diagnosis of birch pollen-related OAS. SN - 1879-1476 UR - https://www.unboundmedicine.com/medline/citation/27816237/Clinical_benefit_of_component_resolved_diagnosis_in_Japanese_birch_allergic_patients_with_a_convincing_history_of_apple_or_peach_allergy_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0385-8146(16)30452-7 DB - PRIME DP - Unbound Medicine ER -