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Haematological and immune upshots in Clarias batrachus exposed to dimethoate and defying response of dietary ascorbic acid.
Chemosphere. 2017 Feb; 168:988-995.C

Abstract

Dimethoate (DM) is an organophosphate insecticide used worldwide in agriculture, household practices. It has resulted in a series of environmental and toxicological impacts on non-target aquatic organisms. The present study investigated the potential ameliorative effects of dietary ascorbic acid (AA) against dimethoate toxicity in the haematological and immune parameters in Clarias batrachus. The experiment included group A (basal diet), group B (basal diet with 1.245 mg L-1 DM) and group C (200 mg kg-1 AA with 1.245 mg L-1 DM) were fed for 8 weeks. Samples were collected at the end of every week in each group and estimated haematological profile (red blood cell count, haemoglobin concentration, haematocrit %, albumin and globulin levels), erythrocyte indices (mean corpuscular volume, mean corpuscular haemoglobin, and mean corpuscular haemoglobin concentration), biochemical parameters (AA levels in blood and liver, total proteins, glucose, serum triglycerides, creatinine levels and glutamic oxaloacetate, glutamic pyruvate transaminase (GOT, GPT)) and immune responses (white blood cell count, serum IgM levels and activities of nitroblue tetrazolium, lysozyme and peroxidase) of the fish. Fish fed with ascorbic acid, showed red blood cell, haemoglobin, haematocrit, erythrocyte indices, albumin, globulin and serum triglycerides, creatinine, plasma total proteins, glucose levels are not differed (≤10%) from control. Likewise, ascorbic acid maintains optimal levels in activities of GOT, GPT, nitroblue tetrazolium, lysozyme and peroxidase, white blood cells and serum IgM levels. Further studies are needed to ascertain how ascorbic acid improves the innate and humoral immune system of the fish and the mechanisms involved.

Authors+Show Affiliations

Department of Zoology, Osmania University, Hyderabad, Telangana, 500 007, India. Electronic address: narramadhusudan@gmail.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27816289

Citation

Narra, Madhusudan Reddy. "Haematological and Immune Upshots in Clarias Batrachus Exposed to Dimethoate and Defying Response of Dietary Ascorbic Acid." Chemosphere, vol. 168, 2017, pp. 988-995.
Narra MR. Haematological and immune upshots in Clarias batrachus exposed to dimethoate and defying response of dietary ascorbic acid. Chemosphere. 2017;168:988-995.
Narra, M. R. (2017). Haematological and immune upshots in Clarias batrachus exposed to dimethoate and defying response of dietary ascorbic acid. Chemosphere, 168, 988-995. https://doi.org/10.1016/j.chemosphere.2016.10.112
Narra MR. Haematological and Immune Upshots in Clarias Batrachus Exposed to Dimethoate and Defying Response of Dietary Ascorbic Acid. Chemosphere. 2017;168:988-995. PubMed PMID: 27816289.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Haematological and immune upshots in Clarias batrachus exposed to dimethoate and defying response of dietary ascorbic acid. A1 - Narra,Madhusudan Reddy, Y1 - 2016/11/02/ PY - 2016/08/12/received PY - 2016/10/24/revised PY - 2016/10/27/accepted PY - 2016/11/7/pubmed PY - 2017/3/4/medline PY - 2016/11/7/entrez KW - Ascorbic acid KW - Dimethoate KW - IgM KW - Immunity KW - Lysozyme KW - Peroxidase SP - 988 EP - 995 JF - Chemosphere JO - Chemosphere VL - 168 N2 - Dimethoate (DM) is an organophosphate insecticide used worldwide in agriculture, household practices. It has resulted in a series of environmental and toxicological impacts on non-target aquatic organisms. The present study investigated the potential ameliorative effects of dietary ascorbic acid (AA) against dimethoate toxicity in the haematological and immune parameters in Clarias batrachus. The experiment included group A (basal diet), group B (basal diet with 1.245 mg L-1 DM) and group C (200 mg kg-1 AA with 1.245 mg L-1 DM) were fed for 8 weeks. Samples were collected at the end of every week in each group and estimated haematological profile (red blood cell count, haemoglobin concentration, haematocrit %, albumin and globulin levels), erythrocyte indices (mean corpuscular volume, mean corpuscular haemoglobin, and mean corpuscular haemoglobin concentration), biochemical parameters (AA levels in blood and liver, total proteins, glucose, serum triglycerides, creatinine levels and glutamic oxaloacetate, glutamic pyruvate transaminase (GOT, GPT)) and immune responses (white blood cell count, serum IgM levels and activities of nitroblue tetrazolium, lysozyme and peroxidase) of the fish. Fish fed with ascorbic acid, showed red blood cell, haemoglobin, haematocrit, erythrocyte indices, albumin, globulin and serum triglycerides, creatinine, plasma total proteins, glucose levels are not differed (≤10%) from control. Likewise, ascorbic acid maintains optimal levels in activities of GOT, GPT, nitroblue tetrazolium, lysozyme and peroxidase, white blood cells and serum IgM levels. Further studies are needed to ascertain how ascorbic acid improves the innate and humoral immune system of the fish and the mechanisms involved. SN - 1879-1298 UR - https://www.unboundmedicine.com/medline/citation/27816289/Haematological_and_immune_upshots_in_Clarias_batrachus_exposed_to_dimethoate_and_defying_response_of_dietary_ascorbic_acid_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0045-6535(16)31503-X DB - PRIME DP - Unbound Medicine ER -