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Constitutive cellulase production from glucose using the recombinant Trichoderma reesei strain overexpressing an artificial transcription activator.
Bioresour Technol. 2017 Jan; 223:317-322.BT

Abstract

The high cost of cellulase production presents biggest challenge in biomass deconstruction. Cellulase production by Trichoderma reesei using low cost carbon source is of great interest. In this study, an artificial transcription activator containing the Cre1 binding domain linked to the Xyr1 effector and binding domains was designed and constitutively overexpressed in T. reesei RUT C30. The recombinant strain T. reesei zxy-2 displayed constitutive cellulase production using glucose as a sole carbon source, and the production titer was 12.75-fold of that observed with T. reesei RUT C30 in shake flask culture. Moreover, FPase and xylanase titers of 2.63 and 108.72IU/mL, respectively, were achieved using glucose as sole carbon source within 48h in a 7-L fermenter by batch fermentation using T. reesei zxy-2. The crude enzyme obtained was used to hydrolyze alkali pretreated corn stover, and a high glucose yield of 99.18% was achieved.

Authors+Show Affiliations

School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116023, China.School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116023, China.State Key Laboratory of Microbial Metabolism & School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address: xqzhao@sjtu.edu.cn.School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116023, China; State Key Laboratory of Microbial Metabolism & School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27818160

Citation

Zhang, Xiaoyue, et al. "Constitutive Cellulase Production From Glucose Using the Recombinant Trichoderma Reesei Strain Overexpressing an Artificial Transcription Activator." Bioresource Technology, vol. 223, 2017, pp. 317-322.
Zhang X, Li Y, Zhao X, et al. Constitutive cellulase production from glucose using the recombinant Trichoderma reesei strain overexpressing an artificial transcription activator. Bioresour Technol. 2017;223:317-322.
Zhang, X., Li, Y., Zhao, X., & Bai, F. (2017). Constitutive cellulase production from glucose using the recombinant Trichoderma reesei strain overexpressing an artificial transcription activator. Bioresource Technology, 223, 317-322. https://doi.org/10.1016/j.biortech.2016.10.083
Zhang X, et al. Constitutive Cellulase Production From Glucose Using the Recombinant Trichoderma Reesei Strain Overexpressing an Artificial Transcription Activator. Bioresour Technol. 2017;223:317-322. PubMed PMID: 27818160.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Constitutive cellulase production from glucose using the recombinant Trichoderma reesei strain overexpressing an artificial transcription activator. AU - Zhang,Xiaoyue, AU - Li,Yonghao, AU - Zhao,Xinqing, AU - Bai,Fengwu, Y1 - 2016/10/31/ PY - 2016/08/15/received PY - 2016/10/25/revised PY - 2016/10/27/accepted PY - 2016/11/8/pubmed PY - 2017/1/10/medline PY - 2016/11/8/entrez KW - Artificial transcription factor KW - Cellulase production KW - Glucose KW - Trichoderma reesei KW - xyr1 and cre1 SP - 317 EP - 322 JF - Bioresource technology JO - Bioresour. Technol. VL - 223 N2 - The high cost of cellulase production presents biggest challenge in biomass deconstruction. Cellulase production by Trichoderma reesei using low cost carbon source is of great interest. In this study, an artificial transcription activator containing the Cre1 binding domain linked to the Xyr1 effector and binding domains was designed and constitutively overexpressed in T. reesei RUT C30. The recombinant strain T. reesei zxy-2 displayed constitutive cellulase production using glucose as a sole carbon source, and the production titer was 12.75-fold of that observed with T. reesei RUT C30 in shake flask culture. Moreover, FPase and xylanase titers of 2.63 and 108.72IU/mL, respectively, were achieved using glucose as sole carbon source within 48h in a 7-L fermenter by batch fermentation using T. reesei zxy-2. The crude enzyme obtained was used to hydrolyze alkali pretreated corn stover, and a high glucose yield of 99.18% was achieved. SN - 1873-2976 UR - https://www.unboundmedicine.com/medline/citation/27818160/Constitutive_cellulase_production_from_glucose_using_the_recombinant_Trichoderma_reesei_strain_overexpressing_an_artificial_transcription_activator_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0960-8524(16)31497-3 DB - PRIME DP - Unbound Medicine ER -