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Methanol extract of Nymphaea lotus ameliorates carbon tetrachloride-induced chronic liver injury in rats via inhibition of oxidative stress.
J Basic Clin Physiol Pharmacol. 2017 Jan 01; 28(1):43-50.JB

Abstract

BACKGROUND

Nymphaea lotus (NL) is an aquatic perennial plant used traditionally in the management of various liver diseases. In this study, the protective effect of methanol extract of NL against carbon tetrachloride (CCl4)-induced chronic hepatotoxicity in rats was investigated.

METHODS

Male Wistar rats were assigned into six groups of five rats each. Group I received corn oil (0.5 mL p.o.) and served as control, group II received CCl4 (1 mL/kg i.p., 1:3 in corn oil), group III received NL (200 mg/kg), and groups IV, V, and VI received CCl4+NL (50, 100, and 200 mg/kg, respectively) for 6 weeks. Twenty-four hours after the last exposure, rats were bled and killed.

RESULTS

The activities of alanine aminotransaminase (ALT), aspartate aminotransferase (AST), and levels of total bilirubin (TB) in the serum, thiobarbituric acid reactive substances (TBARS), superoxide dismutase, catalase, glutathione peroxidase (GPx) and glutathione (GSH) in the liver, and histopathology of the liver were determined using standard procedures. NL significantly (p<0.05) lowered the levels of ALT, AST, and TB and exhibited antioxidant potentials in rats exposed to CCl4 relative to the control values. Specifically, NL at 100 and 200 mg/kg significantly (p<0.05) increased CCl4-induced decrease in hepatic GSH and GPx and also decreased the level of hepatic TBARS in CCl4-intoxicated rats. Histopathological findings revealed cellular infiltration and fibrosis in rats that received CCl4 only, which were ameliorated in rats that received NL+CCl4.

CONCLUSIONS

The data suggest that NL exhibited hepatoprotective effects in CCl4-intoxicated rats via antioxidative mechanism.

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Publisher Full Text (DOI)

Authors

Oyeyemi IT
No affiliation info available
Akanni OO
No affiliation info available
Adaramoye OA
No affiliation info available
Bakare AA
No affiliation info available

MeSH

AnimalsCarbon TetrachlorideChemical and Drug Induced Liver InjuryMaleMethanolNymphaeaOxidative StressPlant ExtractsRatsRats, Wistar

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27824613

Citation

Oyeyemi, Ifeoluwa T., et al. "Methanol Extract of Nymphaea Lotus Ameliorates Carbon Tetrachloride-induced Chronic Liver Injury in Rats Via Inhibition of Oxidative Stress." Journal of Basic and Clinical Physiology and Pharmacology, vol. 28, no. 1, 2017, pp. 43-50.
Oyeyemi IT, Akanni OO, Adaramoye OA, et al. Methanol extract of Nymphaea lotus ameliorates carbon tetrachloride-induced chronic liver injury in rats via inhibition of oxidative stress. J Basic Clin Physiol Pharmacol. 2017;28(1):43-50.
Oyeyemi, I. T., Akanni, O. O., Adaramoye, O. A., & Bakare, A. A. (2017). Methanol extract of Nymphaea lotus ameliorates carbon tetrachloride-induced chronic liver injury in rats via inhibition of oxidative stress. Journal of Basic and Clinical Physiology and Pharmacology, 28(1), 43-50. https://doi.org/10.1515/jbcpp-2016-0029
Oyeyemi IT, et al. Methanol Extract of Nymphaea Lotus Ameliorates Carbon Tetrachloride-induced Chronic Liver Injury in Rats Via Inhibition of Oxidative Stress. J Basic Clin Physiol Pharmacol. 2017 Jan 1;28(1):43-50. PubMed PMID: 27824613.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Methanol extract of Nymphaea lotus ameliorates carbon tetrachloride-induced chronic liver injury in rats via inhibition of oxidative stress. AU - Oyeyemi,Ifeoluwa T, AU - Akanni,Olubukola O, AU - Adaramoye,Oluwatosin A, AU - Bakare,Adekunle A, PY - 2016/03/04/received PY - 2016/08/05/accepted PY - 2016/11/9/pubmed PY - 2017/3/23/medline PY - 2016/11/9/entrez SP - 43 EP - 50 JF - Journal of basic and clinical physiology and pharmacology JO - J Basic Clin Physiol Pharmacol VL - 28 IS - 1 N2 - BACKGROUND: Nymphaea lotus (NL) is an aquatic perennial plant used traditionally in the management of various liver diseases. In this study, the protective effect of methanol extract of NL against carbon tetrachloride (CCl4)-induced chronic hepatotoxicity in rats was investigated. METHODS: Male Wistar rats were assigned into six groups of five rats each. Group I received corn oil (0.5 mL p.o.) and served as control, group II received CCl4 (1 mL/kg i.p., 1:3 in corn oil), group III received NL (200 mg/kg), and groups IV, V, and VI received CCl4+NL (50, 100, and 200 mg/kg, respectively) for 6 weeks. Twenty-four hours after the last exposure, rats were bled and killed. RESULTS: The activities of alanine aminotransaminase (ALT), aspartate aminotransferase (AST), and levels of total bilirubin (TB) in the serum, thiobarbituric acid reactive substances (TBARS), superoxide dismutase, catalase, glutathione peroxidase (GPx) and glutathione (GSH) in the liver, and histopathology of the liver were determined using standard procedures. NL significantly (p<0.05) lowered the levels of ALT, AST, and TB and exhibited antioxidant potentials in rats exposed to CCl4 relative to the control values. Specifically, NL at 100 and 200 mg/kg significantly (p<0.05) increased CCl4-induced decrease in hepatic GSH and GPx and also decreased the level of hepatic TBARS in CCl4-intoxicated rats. Histopathological findings revealed cellular infiltration and fibrosis in rats that received CCl4 only, which were ameliorated in rats that received NL+CCl4. CONCLUSIONS: The data suggest that NL exhibited hepatoprotective effects in CCl4-intoxicated rats via antioxidative mechanism. SN - 2191-0286 UR - https://www.unboundmedicine.com/medline/citation/27824613/Methanol_extract_of_Nymphaea_lotus_ameliorates_carbon_tetrachloride_induced_chronic_liver_injury_in_rats_via_inhibition_of_oxidative_stress_ DB - PRIME DP - Unbound Medicine ER -