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Chromosomal Localization of Genes Conferring Desirable Agronomic Traits from Wheat-Agropyron cristatum Disomic Addition Line 5113.
PLoS One. 2016; 11(11):e0165957.Plos

Abstract

Creation of wheat-alien disomic addition lines and localization of desirable genes on alien chromosomes are important for utilization of these genes in genetic improvement of common wheat. In this study, wheat-Agropyron cristatum derivative line 5113 was characterized by genomic in situ hybridization (GISH) and specific-locus amplified fragment sequencing (SLAF-seq), and was demonstrated to be a novel wheat-A. cristatum disomic 6P addition line. Compared with its parent Fukuhokomugi (Fukuho), 5113 displayed multiple elite agronomic traits, including higher uppermost internode/plant height ratio, larger flag leaf, longer spike length, elevated grain number per spike and spikelet number per spike, more kernel number in the middle spikelet, more fertile tiller number per plant, and enhanced resistance to powdery mildew and leaf rust. Genes conferring these elite traits were localized on the A. cristatum 6P chromosome by using SLAF-seq markers and biparental populations (F1, BC1F1 and BC1F2 populations) produced from the crosses between Fukuho and 5113. Taken together, chromosomal localization of these desirable genes will facilitate transferring of high-yield and high-resistance genes from A. cristatum into common wheat, and serve as the foundation for the utilization of 5113 in wheat breeding.

Authors+Show Affiliations

College of Agronomy, Northwest A&F University, Yangling, Shaanxi, 712100, China. National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.College of Agronomy, Northwest A&F University, Yangling, Shaanxi, 712100, China.National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27824906

Citation

Li, Qingfeng, et al. "Chromosomal Localization of Genes Conferring Desirable Agronomic Traits From Wheat-Agropyron Cristatum Disomic Addition Line 5113." PloS One, vol. 11, no. 11, 2016, pp. e0165957.
Li Q, Lu Y, Pan C, et al. Chromosomal Localization of Genes Conferring Desirable Agronomic Traits from Wheat-Agropyron cristatum Disomic Addition Line 5113. PLoS One. 2016;11(11):e0165957.
Li, Q., Lu, Y., Pan, C., Yao, M., Zhang, J., Yang, X., Liu, W., Li, X., Xi, Y., & Li, L. (2016). Chromosomal Localization of Genes Conferring Desirable Agronomic Traits from Wheat-Agropyron cristatum Disomic Addition Line 5113. PloS One, 11(11), e0165957. https://doi.org/10.1371/journal.pone.0165957
Li Q, et al. Chromosomal Localization of Genes Conferring Desirable Agronomic Traits From Wheat-Agropyron Cristatum Disomic Addition Line 5113. PLoS One. 2016;11(11):e0165957. PubMed PMID: 27824906.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Chromosomal Localization of Genes Conferring Desirable Agronomic Traits from Wheat-Agropyron cristatum Disomic Addition Line 5113. AU - Li,Qingfeng, AU - Lu,Yuqing, AU - Pan,Cuili, AU - Yao,Miaomiao, AU - Zhang,Jinpeng, AU - Yang,Xinming, AU - Liu,Weihua, AU - Li,Xiuquan, AU - Xi,Yajun, AU - Li,Lihui, Y1 - 2016/11/08/ PY - 2016/07/05/received PY - 2016/10/20/accepted PY - 2016/11/9/entrez PY - 2016/11/9/pubmed PY - 2017/7/7/medline SP - e0165957 EP - e0165957 JF - PloS one JO - PLoS One VL - 11 IS - 11 N2 - Creation of wheat-alien disomic addition lines and localization of desirable genes on alien chromosomes are important for utilization of these genes in genetic improvement of common wheat. In this study, wheat-Agropyron cristatum derivative line 5113 was characterized by genomic in situ hybridization (GISH) and specific-locus amplified fragment sequencing (SLAF-seq), and was demonstrated to be a novel wheat-A. cristatum disomic 6P addition line. Compared with its parent Fukuhokomugi (Fukuho), 5113 displayed multiple elite agronomic traits, including higher uppermost internode/plant height ratio, larger flag leaf, longer spike length, elevated grain number per spike and spikelet number per spike, more kernel number in the middle spikelet, more fertile tiller number per plant, and enhanced resistance to powdery mildew and leaf rust. Genes conferring these elite traits were localized on the A. cristatum 6P chromosome by using SLAF-seq markers and biparental populations (F1, BC1F1 and BC1F2 populations) produced from the crosses between Fukuho and 5113. Taken together, chromosomal localization of these desirable genes will facilitate transferring of high-yield and high-resistance genes from A. cristatum into common wheat, and serve as the foundation for the utilization of 5113 in wheat breeding. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/27824906/Chromosomal_Localization_of_Genes_Conferring_Desirable_Agronomic_Traits_from_Wheat_Agropyron_cristatum_Disomic_Addition_Line_5113_ DB - PRIME DP - Unbound Medicine ER -