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Characterization of anti-MERS-CoV antibodies against various recombinant structural antigens of MERS-CoV in an imported case in China.
Emerg Microbes Infect. 2016 Nov 09; 5(11):e113.EM

Abstract

The first imported case of Middle East respiratory syndrome (MERS) in China recently occurred, allowing for the characterization of antibody titers in a series of the patient's sera using the following methods based on recombinant viral structural antigens: inactivated MERS coronavirus (MERS-CoV) enzyme-linked immunosorbent assay (ELISA), recombinant MERS-CoV spike (S, or fragments of S) ELISA, nucleoprotein (NP) ELISA and MERS S pseudovirus particle-based neutralization test (ppNT). A longitudinal profile of the infection showed that seroconversion detected by ELISAs based on the recombinant extracellular domain, S, S1 and receptor-binding domain (RBD) antigens occurred as early as neutralizing antibodies were detected by the ppNT and earlier than antibodies were detected by the inactivated MERS-CoV and N-terminal domain (NTD) ELISAs. Antibodies detected by the NP ELISA occurred last. Strong correlations were found between the S1, RBD and NP ELISAs and the inactivated MERS-CoV ELISA. The S and RBD ELISAs were highly correlated with the commercial S1 ELISA. The S ELISA strongly correlated with the ppNT, although the MERS-CoV, S1, NTD and RBD ELISAs were also significantly correlated with the ppNT (P<0.001).

Authors+Show Affiliations

Key Laboratory of Medical Virology, Ministry of Health, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.Key Laboratory of Medical Virology, Ministry of Health, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.Key Laboratory of Medical Virology, Ministry of Health, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.Guangdong Provincial Center for Disease Control and Prevention, Guangzhou 511430, Guangdong Province, China.Key Laboratory of Medical Virology, Ministry of Health, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.Key Laboratory of Medical Virology, Ministry of Health, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.Guangdong Provincial Center for Disease Control and Prevention, Guangzhou 511430, Guangdong Province, China.Key Laboratory of Medical Virology, Ministry of Health, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.

Pub Type(s)

Case Reports
Journal Article

Language

eng

PubMed ID

27826140

Citation

Wang, Wenling, et al. "Characterization of anti-MERS-CoV Antibodies Against Various Recombinant Structural Antigens of MERS-CoV in an Imported Case in China." Emerging Microbes & Infections, vol. 5, no. 11, 2016, pp. e113.
Wang W, Wang H, Deng Y, et al. Characterization of anti-MERS-CoV antibodies against various recombinant structural antigens of MERS-CoV in an imported case in China. Emerg Microbes Infect. 2016;5(11):e113.
Wang, W., Wang, H., Deng, Y., Song, T., Lan, J., Wu, G., Ke, C., & Tan, W. (2016). Characterization of anti-MERS-CoV antibodies against various recombinant structural antigens of MERS-CoV in an imported case in China. Emerging Microbes & Infections, 5(11), e113. https://doi.org/10.1038/emi.2016.114
Wang W, et al. Characterization of anti-MERS-CoV Antibodies Against Various Recombinant Structural Antigens of MERS-CoV in an Imported Case in China. Emerg Microbes Infect. 2016 Nov 9;5(11):e113. PubMed PMID: 27826140.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of anti-MERS-CoV antibodies against various recombinant structural antigens of MERS-CoV in an imported case in China. AU - Wang,Wenling, AU - Wang,Huijuan, AU - Deng,Yao, AU - Song,Tie, AU - Lan,Jiaming, AU - Wu,Guizhen, AU - Ke,Changwen, AU - Tan,Wenjie, Y1 - 2016/11/09/ PY - 2016/06/14/received PY - 2016/08/09/revised PY - 2016/09/04/accepted PY - 2016/11/10/entrez PY - 2016/11/9/pubmed PY - 2017/2/1/medline SP - e113 EP - e113 JF - Emerging microbes & infections JO - Emerg Microbes Infect VL - 5 IS - 11 N2 - The first imported case of Middle East respiratory syndrome (MERS) in China recently occurred, allowing for the characterization of antibody titers in a series of the patient's sera using the following methods based on recombinant viral structural antigens: inactivated MERS coronavirus (MERS-CoV) enzyme-linked immunosorbent assay (ELISA), recombinant MERS-CoV spike (S, or fragments of S) ELISA, nucleoprotein (NP) ELISA and MERS S pseudovirus particle-based neutralization test (ppNT). A longitudinal profile of the infection showed that seroconversion detected by ELISAs based on the recombinant extracellular domain, S, S1 and receptor-binding domain (RBD) antigens occurred as early as neutralizing antibodies were detected by the ppNT and earlier than antibodies were detected by the inactivated MERS-CoV and N-terminal domain (NTD) ELISAs. Antibodies detected by the NP ELISA occurred last. Strong correlations were found between the S1, RBD and NP ELISAs and the inactivated MERS-CoV ELISA. The S and RBD ELISAs were highly correlated with the commercial S1 ELISA. The S ELISA strongly correlated with the ppNT, although the MERS-CoV, S1, NTD and RBD ELISAs were also significantly correlated with the ppNT (P<0.001). SN - 2222-1751 UR - https://www.unboundmedicine.com/medline/citation/27826140/Characterization_of_anti_MERS_CoV_antibodies_against_various_recombinant_structural_antigens_of_MERS_CoV_in_an_imported_case_in_China_ L2 - https://www.tandfonline.com/doi/full/10.1038/emi.2016.114 DB - PRIME DP - Unbound Medicine ER -