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Cedratvirus, a Double-Cork Structured Giant Virus, is a Distant Relative of Pithoviruses.
Viruses. 2016 11 03; 8(11)V

Abstract

Most viruses are known for the ability to cause symptomatic diseases in humans and other animals. The discovery of Acanthamoeba polyphaga mimivirus and other giant amoebal viruses revealed a considerable and previously unknown area of uncharacterized viral particles. Giant viruses have been isolated from various environmental samples collected from very distant geographic places, revealing a ubiquitous distribution. Their morphological and genomic features are fundamental elements for classifying them. Herein, we report the isolation and draft genome of Cedratvirus, a new amoebal giant virus isolated in Acanthamoeba castellanii, from an Algerian environmental sample. The viral particles are ovoid-shaped, resembling Pithovirus sibericum, but differing notably in the presence of two corks at each extremity of the virion. The draft genome of Cedratvirus-589,068 base pairs in length-is a close relative of the two previously described pithoviruses, sharing 104 and 113 genes with P. sibericum and Pithovirus massiliensis genomes, respectively. Interestingly, analysis of these viruses' core genome reveals that only 21% of Cedratvirus genes are involved in best reciprocal hits with the two pithoviruses. Phylogeny reconstructions and comparative genomics indicate that Cedratvirus is most closely related to pithoviruses, and questions their membership in an enlarged putative Pithoviridae family.

Authors+Show Affiliations

Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE), UM63 CNRS 7278 IRD 198 INSERM U1095, Facultés de Médecine et de Pharmacie, 13385 cedex 05 Marseille, France. miaguiabidou@gmail.com.Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE), UM63 CNRS 7278 IRD 198 INSERM U1095, Facultés de Médecine et de Pharmacie, 13385 cedex 05 Marseille, France. aherfi.s@gmail.com. Institut Hospitalo-Universitaire (IHU) Méditerranée Infection, Pôle des Maladies Infectieuses et Tropicales Clinique et Biologique, Fédération de Bactériologie-Hygiène-Virologie, Centre Hospitalo-Universitaire Timone, Assistance Publique-Hôpitaux de Marseille,13005 Marseille, France. aherfi.s@gmail.com.Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE), UM63 CNRS 7278 IRD 198 INSERM U1095, Facultés de Médecine et de Pharmacie, 13385 cedex 05 Marseille, France. boukhaliljacques@gmail.com.Institut Hospitalo-Universitaire (IHU) Méditerranée Infection, Pôle des Maladies Infectieuses et Tropicales Clinique et Biologique, Fédération de Bactériologie-Hygiène-Virologie, Centre Hospitalo-Universitaire Timone, Assistance Publique-Hôpitaux de Marseille,13005 Marseille, France. tem.timone@gmail.com.Laboratoire Biodiversité et Environnement: Interactions Génomes, Faculté des Sciences Biologiques Université des Sciences et de la Technologie Houari Boumediene, BP 32 EL ALIA 16111 Bab Ezzouar Alger, Algeria. idirbitam@gmail.com.Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE), UM63 CNRS 7278 IRD 198 INSERM U1095, Facultés de Médecine et de Pharmacie, 13385 cedex 05 Marseille, France. didier.raoult@gmail.com. Institut Hospitalo-Universitaire (IHU) Méditerranée Infection, Pôle des Maladies Infectieuses et Tropicales Clinique et Biologique, Fédération de Bactériologie-Hygiène-Virologie, Centre Hospitalo-Universitaire Timone, Assistance Publique-Hôpitaux de Marseille,13005 Marseille, France. didier.raoult@gmail.com.Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE), UM63 CNRS 7278 IRD 198 INSERM U1095, Facultés de Médecine et de Pharmacie, 13385 cedex 05 Marseille, France. philippe.colson@univ-amu.fr. Institut Hospitalo-Universitaire (IHU) Méditerranée Infection, Pôle des Maladies Infectieuses et Tropicales Clinique et Biologique, Fédération de Bactériologie-Hygiène-Virologie, Centre Hospitalo-Universitaire Timone, Assistance Publique-Hôpitaux de Marseille,13005 Marseille, France. philippe.colson@univ-amu.fr.Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE), UM63 CNRS 7278 IRD 198 INSERM U1095, Facultés de Médecine et de Pharmacie, 13385 cedex 05 Marseille, France. bernard.la-scola@univ-amu.fr. Institut Hospitalo-Universitaire (IHU) Méditerranée Infection, Pôle des Maladies Infectieuses et Tropicales Clinique et Biologique, Fédération de Bactériologie-Hygiène-Virologie, Centre Hospitalo-Universitaire Timone, Assistance Publique-Hôpitaux de Marseille,13005 Marseille, France. bernard.la-scola@univ-amu.fr.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27827884

Citation

Andreani, Julien, et al. "Cedratvirus, a Double-Cork Structured Giant Virus, Is a Distant Relative of Pithoviruses." Viruses, vol. 8, no. 11, 2016.
Andreani J, Aherfi S, Bou Khalil JY, et al. Cedratvirus, a Double-Cork Structured Giant Virus, is a Distant Relative of Pithoviruses. Viruses. 2016;8(11).
Andreani, J., Aherfi, S., Bou Khalil, J. Y., Di Pinto, F., Bitam, I., Raoult, D., Colson, P., & La Scola, B. (2016). Cedratvirus, a Double-Cork Structured Giant Virus, is a Distant Relative of Pithoviruses. Viruses, 8(11).
Andreani J, et al. Cedratvirus, a Double-Cork Structured Giant Virus, Is a Distant Relative of Pithoviruses. Viruses. 2016 11 3;8(11) PubMed PMID: 27827884.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cedratvirus, a Double-Cork Structured Giant Virus, is a Distant Relative of Pithoviruses. AU - Andreani,Julien, AU - Aherfi,Sarah, AU - Bou Khalil,Jacques Yaacoub, AU - Di Pinto,Fabrizio, AU - Bitam,Idir, AU - Raoult,Didier, AU - Colson,Philippe, AU - La Scola,Bernard, Y1 - 2016/11/03/ PY - 2016/10/06/received PY - 2016/10/24/revised PY - 2016/10/26/accepted PY - 2016/11/10/entrez PY - 2016/11/10/pubmed PY - 2017/9/14/medline KW - Acanthamoeba KW - Cedratvirus KW - Pithovirus KW - co-culture KW - double-cork KW - giant viruses KW - viral family JF - Viruses JO - Viruses VL - 8 IS - 11 N2 - Most viruses are known for the ability to cause symptomatic diseases in humans and other animals. The discovery of Acanthamoeba polyphaga mimivirus and other giant amoebal viruses revealed a considerable and previously unknown area of uncharacterized viral particles. Giant viruses have been isolated from various environmental samples collected from very distant geographic places, revealing a ubiquitous distribution. Their morphological and genomic features are fundamental elements for classifying them. Herein, we report the isolation and draft genome of Cedratvirus, a new amoebal giant virus isolated in Acanthamoeba castellanii, from an Algerian environmental sample. The viral particles are ovoid-shaped, resembling Pithovirus sibericum, but differing notably in the presence of two corks at each extremity of the virion. The draft genome of Cedratvirus-589,068 base pairs in length-is a close relative of the two previously described pithoviruses, sharing 104 and 113 genes with P. sibericum and Pithovirus massiliensis genomes, respectively. Interestingly, analysis of these viruses' core genome reveals that only 21% of Cedratvirus genes are involved in best reciprocal hits with the two pithoviruses. Phylogeny reconstructions and comparative genomics indicate that Cedratvirus is most closely related to pithoviruses, and questions their membership in an enlarged putative Pithoviridae family. SN - 1999-4915 UR - https://www.unboundmedicine.com/medline/citation/27827884/Cedratvirus_a_Double_Cork_Structured_Giant_Virus_is_a_Distant_Relative_of_Pithoviruses_ L2 - https://www.mdpi.com/resolver?pii=v8110300 DB - PRIME DP - Unbound Medicine ER -