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Multiplex assay development and mutation rate analysis for 13 RM Y-STRs in Chinese Han population.
Int J Legal Med. 2017 Mar; 131(2):345-350.IJ

Abstract

In this study, a novel multiplex polymerase chain reaction (PCR) assay was developed for amplifying the newly introduced 13 rapidly mutating Y-STR markers (RM Y-STRs) including DYF387S1, DYF399S1, DYF403S1a/b, DYF404S1, DYS449, DYS518, DYS526b, DYS547, DYS570, DYS576, DYS612, DYS626, and DYS627. In addition, a survey for mutation rates of the 13 RM Y-STRs in Chinese Han population was performed to make sure of the mutation characteristic and application in Chinese group. With 14,476 allele transfers in 1034 father-son pairs, 221 mutation events occurred, of which 215 were one-step mutations and 6 were two-step mutations. Nineteen father-son pairs were found to have mutations at two loci and one pair at three loci. Based upon our research data, 18.96 % of all 1034 father-son pairs were successfully differentiated, and the estimated locus-specific mutation rates varied from 4.84 × 10-3 to 6.29 × 10-2, with an average estimated mutation rate 1.53 × 10-2 (95 % CI 1.33 × 10-2 to 1.74 × 10-2). Among the 13 Y-STR markers, eight loci (DYF399S1, DYF403S1a, DYF404S1, DYS449, DYS518, DYS547, DYS576, and DYS612) had mutation rates higher than 1.00 × 10-2, and the rest loci lower than 1.00 × 10-2 in Chinese samples.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Zhang W
Department of Forensic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, People's Republic of China. Qingdao Licang District People's Procuratorate, Qingdao, 266100, People's Republic of China.
Xiao C
Department of Forensic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, People's Republic of China.
Yu J
Wuhan Blood Center, Wuhan, 430033, People's Republic of China.
Wei T
Department of Forensic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, People's Republic of China.
Liao F
Department of Forensic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, People's Republic of China.
Wei W
Department of Forensic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, People's Republic of China.
Huang D
Department of Forensic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, People's Republic of China. huangdaixin@mails.tjmu.edu.cn.

MeSH

ChinaChromosomes, Human, YDNA FingerprintingEthnicityGenetic MarkersGenetics, PopulationHumansMaleMicrosatellite RepeatsMultiplex Polymerase Chain ReactionMutation Rate

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27830338

Citation

Zhang, Wenqiong, et al. "Multiplex Assay Development and Mutation Rate Analysis for 13 RM Y-STRs in Chinese Han Population." International Journal of Legal Medicine, vol. 131, no. 2, 2017, pp. 345-350.
Zhang W, Xiao C, Yu J, et al. Multiplex assay development and mutation rate analysis for 13 RM Y-STRs in Chinese Han population. Int J Legal Med. 2017;131(2):345-350.
Zhang, W., Xiao, C., Yu, J., Wei, T., Liao, F., Wei, W., & Huang, D. (2017). Multiplex assay development and mutation rate analysis for 13 RM Y-STRs in Chinese Han population. International Journal of Legal Medicine, 131(2), 345-350. https://doi.org/10.1007/s00414-016-1489-y
Zhang W, et al. Multiplex Assay Development and Mutation Rate Analysis for 13 RM Y-STRs in Chinese Han Population. Int J Legal Med. 2017;131(2):345-350. PubMed PMID: 27830338.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Multiplex assay development and mutation rate analysis for 13 RM Y-STRs in Chinese Han population. AU - Zhang,Wenqiong, AU - Xiao,Chao, AU - Yu,Jin, AU - Wei,Tian, AU - Liao,Fei, AU - Wei,Wei, AU - Huang,Daixin, Y1 - 2016/11/09/ PY - 2016/09/08/received PY - 2016/10/25/accepted PY - 2016/11/11/pubmed PY - 2017/10/24/medline PY - 2016/11/11/entrez KW - Lineage differentiation KW - Multiplex assay KW - Mutation KW - Rapidly mutating Y-STR SP - 345 EP - 350 JF - International journal of legal medicine JO - Int J Legal Med VL - 131 IS - 2 N2 - In this study, a novel multiplex polymerase chain reaction (PCR) assay was developed for amplifying the newly introduced 13 rapidly mutating Y-STR markers (RM Y-STRs) including DYF387S1, DYF399S1, DYF403S1a/b, DYF404S1, DYS449, DYS518, DYS526b, DYS547, DYS570, DYS576, DYS612, DYS626, and DYS627. In addition, a survey for mutation rates of the 13 RM Y-STRs in Chinese Han population was performed to make sure of the mutation characteristic and application in Chinese group. With 14,476 allele transfers in 1034 father-son pairs, 221 mutation events occurred, of which 215 were one-step mutations and 6 were two-step mutations. Nineteen father-son pairs were found to have mutations at two loci and one pair at three loci. Based upon our research data, 18.96 % of all 1034 father-son pairs were successfully differentiated, and the estimated locus-specific mutation rates varied from 4.84 × 10-3 to 6.29 × 10-2, with an average estimated mutation rate 1.53 × 10-2 (95 % CI 1.33 × 10-2 to 1.74 × 10-2). Among the 13 Y-STR markers, eight loci (DYF399S1, DYF403S1a, DYF404S1, DYS449, DYS518, DYS547, DYS576, and DYS612) had mutation rates higher than 1.00 × 10-2, and the rest loci lower than 1.00 × 10-2 in Chinese samples. SN - 1437-1596 UR - https://www.unboundmedicine.com/medline/citation/27830338/Multiplex_assay_development_and_mutation_rate_analysis_for_13_RM_Y_STRs_in_Chinese_Han_population_ DB - PRIME DP - Unbound Medicine ER -