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Isolation and application of P genome-specific DNA sequences of Agropyron Gaertn. in Triticeae.
Planta. 2017 Feb; 245(2):425-437.P

Abstract

MAIN CONCLUSION

Different types of P genome sequences and markers were developed, which could be used to analyze the evolution of P genome in Triticeae and identify precisely wheat- A. cristatum introgression lines. P genome of Agropyron Gaertn. plays an important role in Triticeae and could provide many desirable genes conferring high yield, disease resistance, and stress tolerance for wheat genetic improvement. Therefore, it is significant to develop specific sequences and functional markers of P genome. In this study, 126 sequences were isolated from the degenerate oligonucleotide primed-polymerase chain reaction (DOP-PCR) products of microdissected chromosome 6PS. Forty-eight sequences were identified as P genome-specific sequences by dot-blot hybridization and DNA sequences analysis. Among these sequences, 22 displayed the characteristics of retrotransposons, nine and one displayed the characteristics of DNA transposons and tandem repetitive sequence, respectively. Fourteen of 48 sequences were determined to distribute on different regions of P genome chromosomes by fluorescence in situ hybridization, and the distributing regions were as following: all over P genome chromosomes, centromeres, pericentromeric regions, distal regions, and terminal regions. We compared the P genome sequences with other genome sequences of Triticeae and found that the similar sequences of the P genome sequences were widespread in Triticeae, but differentiation occurred to various extents. Additionally, thirty-four molecular markers were developed from the P genome sequences, which could be used for analyzing the evolutionary relationship among 16 genomes of 18 species in Triticeae and identifying P genome chromatin in wheat-A. cristatum introgression lines. These results will not only facilitate the study of structure and evolution of P genome chromosomes, but also provide a rapid detecting tool for effective utilization of desirable genes of P genome in wheat improvement.

Authors+Show Affiliations

National Key Facility for Crop Gene Resources and Genetic Improvement (NKCRI), Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China. State Key Laboratory for Agrobiotechnology and Key Laboratory of Crop Heterosis and Utilization (MOE) and Key Laboratory of Crop Genomics and Genetic Improvement (MOA), Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing, 100193, China.National Key Facility for Crop Gene Resources and Genetic Improvement (NKCRI), Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.National Key Facility for Crop Gene Resources and Genetic Improvement (NKCRI), Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.National Key Facility for Crop Gene Resources and Genetic Improvement (NKCRI), Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.National Key Facility for Crop Gene Resources and Genetic Improvement (NKCRI), Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.National Key Facility for Crop Gene Resources and Genetic Improvement (NKCRI), Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China. zmhu@genetics.ac.cn.National Key Facility for Crop Gene Resources and Genetic Improvement (NKCRI), Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China. lilihui@caas.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27832372

Citation

Han, Haiming, et al. "Isolation and Application of P Genome-specific DNA Sequences of Agropyron Gaertn. in Triticeae." Planta, vol. 245, no. 2, 2017, pp. 425-437.
Han H, Liu W, Lu Y, et al. Isolation and application of P genome-specific DNA sequences of Agropyron Gaertn. in Triticeae. Planta. 2017;245(2):425-437.
Han, H., Liu, W., Lu, Y., Zhang, J., Yang, X., Li, X., Hu, Z., & Li, L. (2017). Isolation and application of P genome-specific DNA sequences of Agropyron Gaertn. in Triticeae. Planta, 245(2), 425-437. https://doi.org/10.1007/s00425-016-2616-1
Han H, et al. Isolation and Application of P Genome-specific DNA Sequences of Agropyron Gaertn. in Triticeae. Planta. 2017;245(2):425-437. PubMed PMID: 27832372.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Isolation and application of P genome-specific DNA sequences of Agropyron Gaertn. in Triticeae. AU - Han,Haiming, AU - Liu,Weihua, AU - Lu,Yuqing, AU - Zhang,Jinpeng, AU - Yang,Xinming, AU - Li,Xiuquan, AU - Hu,Zanmin, AU - Li,Lihui, Y1 - 2016/11/10/ PY - 2016/09/14/received PY - 2016/10/31/accepted PY - 2016/11/11/pubmed PY - 2017/4/22/medline PY - 2016/11/11/entrez KW - Evolution KW - Molecular marker KW - P genome KW - Specific sequences KW - Wheat-A. cristatum lines SP - 425 EP - 437 JF - Planta JO - Planta VL - 245 IS - 2 N2 - MAIN CONCLUSION: Different types of P genome sequences and markers were developed, which could be used to analyze the evolution of P genome in Triticeae and identify precisely wheat- A. cristatum introgression lines. P genome of Agropyron Gaertn. plays an important role in Triticeae and could provide many desirable genes conferring high yield, disease resistance, and stress tolerance for wheat genetic improvement. Therefore, it is significant to develop specific sequences and functional markers of P genome. In this study, 126 sequences were isolated from the degenerate oligonucleotide primed-polymerase chain reaction (DOP-PCR) products of microdissected chromosome 6PS. Forty-eight sequences were identified as P genome-specific sequences by dot-blot hybridization and DNA sequences analysis. Among these sequences, 22 displayed the characteristics of retrotransposons, nine and one displayed the characteristics of DNA transposons and tandem repetitive sequence, respectively. Fourteen of 48 sequences were determined to distribute on different regions of P genome chromosomes by fluorescence in situ hybridization, and the distributing regions were as following: all over P genome chromosomes, centromeres, pericentromeric regions, distal regions, and terminal regions. We compared the P genome sequences with other genome sequences of Triticeae and found that the similar sequences of the P genome sequences were widespread in Triticeae, but differentiation occurred to various extents. Additionally, thirty-four molecular markers were developed from the P genome sequences, which could be used for analyzing the evolutionary relationship among 16 genomes of 18 species in Triticeae and identifying P genome chromatin in wheat-A. cristatum introgression lines. These results will not only facilitate the study of structure and evolution of P genome chromosomes, but also provide a rapid detecting tool for effective utilization of desirable genes of P genome in wheat improvement. SN - 1432-2048 UR - https://www.unboundmedicine.com/medline/citation/27832372/Isolation_and_application_of_P_genome_specific_DNA_sequences_of_Agropyron_Gaertn__in_Triticeae_ L2 - https://dx.doi.org/10.1007/s00425-016-2616-1 DB - PRIME DP - Unbound Medicine ER -