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Ghrelin improves intestinal mucosal atrophy during parenteral nutrition: An experimental study.
J Pediatr Surg. 2016 Dec; 51(12):2039-2043.JP

Abstract

BACKGROUND/PURPOSE

Total parenteral nutrition (TPN) has been reported to be associated with mucosal atrophy of the small intestine. Ghrelin has hormonal, orexigenic, and metabolic activities. We investigated whether ghrelin improved intestinal mucosal atrophy using a TPN-supported rat model.

METHODS

Rats underwent jugular vein catheterization and were divided into four groups: TPN alone (TPN), TPN plus low-dose ghrelin (TPNLG), TPN plus high-dose ghrelin (TPNHG), and oral feeding with normal chow (OF). Ghrelin was administered continuously at dosages of 10 or 50 μg/kg/day. On day 6 rats were euthanized, and the small intestine was harvested and divided into the jejunum and ileum. Then the villus height (VH) and crypt depth (CD) were evaluated.

RESULTS

The jejunal and ileal VH and CD in the TPN group were significantly decreased compared with those in the OF group. TPNHG improved only VH of the jejunum. TPNLG improved VH and CD of the jejunum and CD of the ileum. The improvement of TPNLG was significantly stronger than that in CD of the jejunum and ileum.

CONCLUSIONS

TPN was more strongly associated with mucosal atrophy in the jejunum than in the ileum. Low-dose intravenous administration of ghrelin improved TPN-associated intestinal mucosal atrophy more effectively than high-dose administration.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Department of Pediatric Surgery, Research Field in Medicine and Health Sciences, Medical and Dental Sciences Area, Research and Education Assembly, Kagoshima University.

Department of Pediatric Surgery, Research Field in Medicine and Health Sciences, Medical and Dental Sciences Area, Research and Education Assembly, Kagoshima University.

Department of Pediatric Surgery, Research Field in Medicine and Health Sciences, Medical and Dental Sciences Area, Research and Education Assembly, Kagoshima University.

Department of Pediatric Surgery, Research Field in Medicine and Health Sciences, Medical and Dental Sciences Area, Research and Education Assembly, Kagoshima University.

Department of Pediatric Surgery, Research Field in Medicine and Health Sciences, Medical and Dental Sciences Area, Research and Education Assembly, Kagoshima University.

Department of Pediatric Surgery, Research Field in Medicine and Health Sciences, Medical and Dental Sciences Area, Research and Education Assembly, Kagoshima University.

Department of Pediatric Surgery, Research Field in Medicine and Health Sciences, Medical and Dental Sciences Area, Research and Education Assembly, Kagoshima University.

Department of Pathology and Oncology, Research Field in Medicine and Health Sciences, Medical and Dental Sciences Area, Research and Education Assembly, Kagoshima University.

National Center for Children Health and Development, Pathology.

Department of Pathology and Oncology, Research Field in Medicine and Health Sciences, Medical and Dental Sciences Area, Research and Education Assembly, Kagoshima University.

Department of Pediatric Surgery, Research Field in Medicine and Health Sciences, Medical and Dental Sciences Area, Research and Education Assembly, Kagoshima University. Electronic address: sieiri@m.kufm.kagoshima-u.ac.jp.

MeSH

AnimalsAtrophyDisease Models, AnimalGastrointestinal AgentsGhrelinIleumInfusions, IntravenousIntestinal MucosaIntestine, SmallJejunumMaleParenteral Nutrition, TotalRatsRats, Sprague-Dawley

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27832865

Citation

Yamada, Waka, et al. "Ghrelin Improves Intestinal Mucosal Atrophy During Parenteral Nutrition: an Experimental Study." Journal of Pediatric Surgery, vol. 51, no. 12, 2016, pp. 2039-2043.

Yamada W, Kaji T, Onishi S, et al. Ghrelin improves intestinal mucosal atrophy during parenteral nutrition: An experimental study. J Pediatr Surg. 2016;51(12):2039-2043.

Yamada, W., Kaji, T., Onishi, S., Nakame, K., Yamada, K., Kawano, T., Mukai, M., Souda, M., Yoshioka, T., Tanimoto, A., & Ieiri, S. (2016). Ghrelin improves intestinal mucosal atrophy during parenteral nutrition: An experimental study. Journal of Pediatric Surgery, 51(12), 2039-2043. https://doi.org/10.1016/j.jpedsurg.2016.09.035

Yamada W, et al. Ghrelin Improves Intestinal Mucosal Atrophy During Parenteral Nutrition: an Experimental Study. J Pediatr Surg. 2016;51(12):2039-2043. PubMed PMID: 27832865.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Ghrelin improves intestinal mucosal atrophy during parenteral nutrition: An experimental study. AU - Yamada,Waka, AU - Kaji,Tatsuru, AU - Onishi,Shun, AU - Nakame,Kazuhiko, AU - Yamada,Koji, AU - Kawano,Takafumi, AU - Mukai,Motoi, AU - Souda,Masakazu, AU - Yoshioka,Takako, AU - Tanimoto,Akihide, AU - Ieiri,Satoshi, Y1 - 2016/09/17/ PY - 2016/08/15/received PY - 2016/09/12/accepted PY - 2016/11/12/pubmed PY - 2017/2/15/medline PY - 2016/11/12/entrez KW - Ghrelin KW - Mucosal atrophy KW - Parenteral nutrition SP - 2039 EP - 2043 JF - Journal of pediatric surgery JO - J Pediatr Surg VL - 51 IS - 12 N2 - BACKGROUND/PURPOSE: Total parenteral nutrition (TPN) has been reported to be associated with mucosal atrophy of the small intestine. Ghrelin has hormonal, orexigenic, and metabolic activities. We investigated whether ghrelin improved intestinal mucosal atrophy using a TPN-supported rat model. METHODS: Rats underwent jugular vein catheterization and were divided into four groups: TPN alone (TPN), TPN plus low-dose ghrelin (TPNLG), TPN plus high-dose ghrelin (TPNHG), and oral feeding with normal chow (OF). Ghrelin was administered continuously at dosages of 10 or 50 μg/kg/day. On day 6 rats were euthanized, and the small intestine was harvested and divided into the jejunum and ileum. Then the villus height (VH) and crypt depth (CD) were evaluated. RESULTS: The jejunal and ileal VH and CD in the TPN group were significantly decreased compared with those in the OF group. TPNHG improved only VH of the jejunum. TPNLG improved VH and CD of the jejunum and CD of the ileum. The improvement of TPNLG was significantly stronger than that in CD of the jejunum and ileum. CONCLUSIONS: TPN was more strongly associated with mucosal atrophy in the jejunum than in the ileum. Low-dose intravenous administration of ghrelin improved TPN-associated intestinal mucosal atrophy more effectively than high-dose administration. SN - 1531-5037 UR - https://www.unboundmedicine.com/medline/citation/27832865/Ghrelin_improves_intestinal_mucosal_atrophy_during_parenteral_nutrition:_An_experimental_study_ DB - PRIME DP - Unbound Medicine ER -