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Probiotic Lactobacillus fermentum UCO-979C biofilm formation on AGS and Caco-2 cells and Helicobacter pylori inhibition.
Biofouling. 2016 11; 32(10):1245-1257.B

Abstract

The ability of the human isolate Lactobacillus fermentum UCO-979C to form biofilm and synthesize exopolysaccharide on abiotic and biotic models is described. These properties were compared with the well-known Lactobacillus casei Shirota to better understand their anti-Helicobacter pylori probiotic activities. The two strains of lactobacilli synthesized exopolysaccharide as detected by the Dubois method and formed biofilm on abiotic and biotic surfaces visualized by crystal violet staining and scanning electron microscopy. Concomitantly, these strains inhibited H. pylori urease activity by up to 80.4% (strain UCO-979C) and 66.8% (strain Shirota) in gastric adenocarcinoma (AGS) cells, but the two species showed equal levels of inhibition (~84%) in colorectal adenocarcinoma (Caco-2) cells. The results suggest that L. fermentum UCO-979C has probiotic potential against H. pylori infections. However, further analyses are needed to explain the increased activity observed against the pathogen in AGS cells as compared to L. casei Shirota.

Authors+Show Affiliations

Salas-Jara MJ
a Laboratorio de Patogenicidad Bacteriana, Departamento de Microbiología, Facultad de Ciencias Biológicas , Universidad de Concepción , Concepción , Chile.
Sanhueza EA
a Laboratorio de Patogenicidad Bacteriana, Departamento de Microbiología, Facultad de Ciencias Biológicas , Universidad de Concepción , Concepción , Chile.
Retamal-Díaz A
b Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas , Pontificia Universidad Católica de Chile , Santiago , Chile.
González C
a Laboratorio de Patogenicidad Bacteriana, Departamento de Microbiología, Facultad de Ciencias Biológicas , Universidad de Concepción , Concepción , Chile.
Urrutia H
c Laboratorio de Investigación de Estructura, Función y Aplicaciones en Biotecnología Ambiental de Biopelículas Microbianas, Centro de Biotecnología , Universidad de Concepción , Concepción , Chile.
García A
a Laboratorio de Patogenicidad Bacteriana, Departamento de Microbiología, Facultad de Ciencias Biológicas , Universidad de Concepción , Concepción , Chile.

MeSH

AntibiosisBacterial AdhesionBiofilmsCaco-2 CellsColonic NeoplasmsHelicobacter pyloriHumansLacticaseibacillus caseiLimosilactobacillus fermentumMicroscopy, Electron, ScanningPolysaccharides, BacterialProbioticsStomach Neoplasms

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27834106

Citation

Salas-Jara, M J., et al. "Probiotic Lactobacillus Fermentum UCO-979C Biofilm Formation On AGS and Caco-2 Cells and Helicobacter Pylori Inhibition." Biofouling, vol. 32, no. 10, 2016, pp. 1245-1257.
Salas-Jara MJ, Sanhueza EA, Retamal-Díaz A, et al. Probiotic Lactobacillus fermentum UCO-979C biofilm formation on AGS and Caco-2 cells and Helicobacter pylori inhibition. Biofouling. 2016;32(10):1245-1257.
Salas-Jara, M. J., Sanhueza, E. A., Retamal-Díaz, A., González, C., Urrutia, H., & García, A. (2016). Probiotic Lactobacillus fermentum UCO-979C biofilm formation on AGS and Caco-2 cells and Helicobacter pylori inhibition. Biofouling, 32(10), 1245-1257.
Salas-Jara MJ, et al. Probiotic Lactobacillus Fermentum UCO-979C Biofilm Formation On AGS and Caco-2 Cells and Helicobacter Pylori Inhibition. Biofouling. 2016;32(10):1245-1257. PubMed PMID: 27834106.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Probiotic Lactobacillus fermentum UCO-979C biofilm formation on AGS and Caco-2 cells and Helicobacter pylori inhibition. AU - Salas-Jara,M J, AU - Sanhueza,E A, AU - Retamal-Díaz,A, AU - González,C, AU - Urrutia,H, AU - García,A, PY - 2016/11/12/entrez PY - 2016/11/12/pubmed PY - 2017/8/17/medline KW - AGS cells KW - Caco-2 cells KW - Helicobacter pylori KW - Lactobacillus fermentum SP - 1245 EP - 1257 JF - Biofouling JO - Biofouling VL - 32 IS - 10 N2 - The ability of the human isolate Lactobacillus fermentum UCO-979C to form biofilm and synthesize exopolysaccharide on abiotic and biotic models is described. These properties were compared with the well-known Lactobacillus casei Shirota to better understand their anti-Helicobacter pylori probiotic activities. The two strains of lactobacilli synthesized exopolysaccharide as detected by the Dubois method and formed biofilm on abiotic and biotic surfaces visualized by crystal violet staining and scanning electron microscopy. Concomitantly, these strains inhibited H. pylori urease activity by up to 80.4% (strain UCO-979C) and 66.8% (strain Shirota) in gastric adenocarcinoma (AGS) cells, but the two species showed equal levels of inhibition (~84%) in colorectal adenocarcinoma (Caco-2) cells. The results suggest that L. fermentum UCO-979C has probiotic potential against H. pylori infections. However, further analyses are needed to explain the increased activity observed against the pathogen in AGS cells as compared to L. casei Shirota. SN - 1029-2454 UR - https://www.unboundmedicine.com/medline/citation/27834106/Probiotic_Lactobacillus_fermentum_UCO_979C_biofilm_formation_on_AGS_and_Caco_2_cells_and_Helicobacter_pylori_inhibition_ DB - PRIME DP - Unbound Medicine ER -