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Pharmacological effects of the phytochemicals of Anethum sowa L. root extracts.
BMC Complement Altern Med. 2016 Nov 14; 16(1):464.BC

Abstract

BACKGROUND

Anethum sowa L. is widely used as an important spice and traditional medicinal plants to treat various ailments. On the basis of scientific ethnobotanical information, this study was undertaken to evaluate the antioxidant, antimicrobial and cytotoxic activity of the crude extracts of Anethum sowa L. roots as well as to identify the classes of phytochemicals by chemical tests.

METHODS

The antioxidant potential of the extracts was ascertained with the stable organic free radical (2, 2-diphenyl-1-picryl-hydrazyl). The agar well diffusion method was used to determine the susceptibility of bacterial and fungal strains of the crude extracts. The minimum inhibitory concentration (MIC) and minimum bactericidal concentrations (MBC) were determined by the microdilution test. Cytotoxic activities were screened using brine shrimps (Artemia salina) lethality assay. Finally, phytochemicals were profiled using standard procedures.

RESULTS

A preliminary phytochemical screening of the different crude extracts by methanol, ethyl acetate and chloroform showed the presence of secondary metabolites such as flavonoids, alkaloids, saponin, cardiac glycosides and tannins while cyanogenetic glycosides were not detected. The methanol, ethyl acetate and chloroform extracts displayed high antioxidant activity (IC50 = 13.08 ± 0.03, 33.48 ± 0.16 and 36.42 ± 0.41 μg/mL, respectively) in the DPPH assay comparable to that of the standard ascorbic acid and BHT (IC50 = 3.74 ± 0.05 and 11.84 ± 0.29 μg/mL). The cytotoxic activity of the crude ethyl acetate and chloroform extracts possessed excellent activity (LC50 = 5.03 ± 0.08, 5.23 ± 0.11 and 17.22 ± 0.14 μg/mL, respectively) against brine shrimp larvae after 24 h of treatment and compared with standard vincristine sulphate (LC50 = 0.46 ± 0.05 μg/mL). The extracts also showed good antimicrobial activity against both Gram-positive and Gram-negative bacteria when compared with two standard antibiotics ciprofloxacin and tetracycline.

CONCLUSION

These results showed that the Anethum sowa root extracts are the important source of the antioxidant, antimicrobial and cytotoxic agent. So, further research is necessary to isolate and characterize of different phytoconstituents for pharmaceutical drug lead molecules and also to verify its traditional uses.

Authors+Show Affiliations

Department of Chemistry, Jahangirnagar University, Savar, 1342, Dhaka, Bangladesh. saleheen.sosthe@gmail.com.Institute of National Analytical Research and Services, BCSIR Laboratories, Bangladesh Council of Scientific and Industrial Research, Dhaka, 1205, Bangladesh.Industrial Microbiology Research Division, Institute of Food Science and Technology, Bangladesh Council of Scientific and Industrial Research, Dhaka, 1205, Bangladesh.Department of Microbiology, Jagannath University, Dhaka, 1100, Bangladesh.Department of Chemistry, Jahangirnagar University, Savar, 1342, Dhaka, Bangladesh.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27842527

Citation

Saleh-E-In, Md Moshfekus, et al. "Pharmacological Effects of the Phytochemicals of Anethum Sowa L. Root Extracts." BMC Complementary and Alternative Medicine, vol. 16, no. 1, 2016, p. 464.
Saleh-E-In MM, Sultana N, Hossain MN, et al. Pharmacological effects of the phytochemicals of Anethum sowa L. root extracts. BMC Complement Altern Med. 2016;16(1):464.
Saleh-E-In, M. M., Sultana, N., Hossain, M. N., Hasan, S., & Islam, M. R. (2016). Pharmacological effects of the phytochemicals of Anethum sowa L. root extracts. BMC Complementary and Alternative Medicine, 16(1), 464. https://doi.org/10.1186/s12906-016-1438-9
Saleh-E-In MM, et al. Pharmacological Effects of the Phytochemicals of Anethum Sowa L. Root Extracts. BMC Complement Altern Med. 2016 Nov 14;16(1):464. PubMed PMID: 27842527.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Pharmacological effects of the phytochemicals of Anethum sowa L. root extracts. AU - Saleh-E-In,Md Moshfekus, AU - Sultana,Nasim, AU - Hossain,Md Nur, AU - Hasan,Sayeema, AU - Islam,Md Rabiul, Y1 - 2016/11/14/ PY - 2016/03/26/received PY - 2016/10/27/accepted PY - 2016/11/16/entrez PY - 2016/11/16/pubmed PY - 2017/2/1/medline KW - Anethum sowa L KW - Antioxidant KW - Cytotoxicity KW - Gram-negative bacteria KW - Gram-positive bacteria KW - Phytochemical screening SP - 464 EP - 464 JF - BMC complementary and alternative medicine JO - BMC Complement Altern Med VL - 16 IS - 1 N2 - BACKGROUND: Anethum sowa L. is widely used as an important spice and traditional medicinal plants to treat various ailments. On the basis of scientific ethnobotanical information, this study was undertaken to evaluate the antioxidant, antimicrobial and cytotoxic activity of the crude extracts of Anethum sowa L. roots as well as to identify the classes of phytochemicals by chemical tests. METHODS: The antioxidant potential of the extracts was ascertained with the stable organic free radical (2, 2-diphenyl-1-picryl-hydrazyl). The agar well diffusion method was used to determine the susceptibility of bacterial and fungal strains of the crude extracts. The minimum inhibitory concentration (MIC) and minimum bactericidal concentrations (MBC) were determined by the microdilution test. Cytotoxic activities were screened using brine shrimps (Artemia salina) lethality assay. Finally, phytochemicals were profiled using standard procedures. RESULTS: A preliminary phytochemical screening of the different crude extracts by methanol, ethyl acetate and chloroform showed the presence of secondary metabolites such as flavonoids, alkaloids, saponin, cardiac glycosides and tannins while cyanogenetic glycosides were not detected. The methanol, ethyl acetate and chloroform extracts displayed high antioxidant activity (IC50 = 13.08 ± 0.03, 33.48 ± 0.16 and 36.42 ± 0.41 μg/mL, respectively) in the DPPH assay comparable to that of the standard ascorbic acid and BHT (IC50 = 3.74 ± 0.05 and 11.84 ± 0.29 μg/mL). The cytotoxic activity of the crude ethyl acetate and chloroform extracts possessed excellent activity (LC50 = 5.03 ± 0.08, 5.23 ± 0.11 and 17.22 ± 0.14 μg/mL, respectively) against brine shrimp larvae after 24 h of treatment and compared with standard vincristine sulphate (LC50 = 0.46 ± 0.05 μg/mL). The extracts also showed good antimicrobial activity against both Gram-positive and Gram-negative bacteria when compared with two standard antibiotics ciprofloxacin and tetracycline. CONCLUSION: These results showed that the Anethum sowa root extracts are the important source of the antioxidant, antimicrobial and cytotoxic agent. So, further research is necessary to isolate and characterize of different phytoconstituents for pharmaceutical drug lead molecules and also to verify its traditional uses. SN - 1472-6882 UR - https://www.unboundmedicine.com/medline/citation/27842527/Pharmacological_effects_of_the_phytochemicals_of_Anethum_sowa_L__root_extracts_ DB - PRIME DP - Unbound Medicine ER -