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N-acetylcysteine protects against star fruit-induced acute kidney injury.
Ren Fail. 2017 11; 39(1):193-202.RF

Abstract

BACKGROUND

Star fruit (SF) is a popular fruit, commonly cultivated in many tropical countries, that contains large amount of oxalate. Acute oxalate nephropathy and direct renal tubular damage through release of free radicals are the main mechanisms involved in SF-induced acute kidney injury (AKI). The aim of this study was to evaluate the protective effect of N-acetylcysteine (NAC) on SF-induced nephrotoxicity due to its potent antioxidant effect.

MATERIALS AND METHODS

Male Wistar rats received SF juice (4 mL/100 g body weight) by gavage after a 12 h fasting and water deprivation. Fasting and water deprivation continued for 6 h thereafter to warrant juice absorption. Thereafter, animals were allocated to three experimental groups: SF (n = 6): received tap water; SF + NAC (n = 6): received NAC (4.8 g/L) in drinking water for 48 h after gavage; and Sham (n = 6): no interventions. After 48 h, inulin clearance studies were performed to determine glomerular filtration rate. In a second series of experiment, rats were housed in metabolic cages for additional assessments.

RESULTS

SF rats showed markedly reduced inulin clearance associated with hyperoxaluria, renal tubular damage, increased oxidative stress and inflammation. NAC treatment ameliorated all these alterations. Under polarized light microscopy, SF rats exhibited intense calcium oxalate birefringence crystals deposition, dilation of renal tubules and tubular epithelial degeneration, which were attenuate by NAC therapy.

CONCLUSIONS

Our data show that therapeutic NAC attenuates renal dysfunction in a model of acute oxalate nephropathy following SF ingestion by reducing oxidative stress, oxaluria, and inflammation. This might represent a novel indication of NAC for the treatment of SF-induced AKI.

Authors+Show Affiliations

a Department of Nephrology, School of Medicine , University of São Paulo , São Paulo , Brazil.a Department of Nephrology, School of Medicine , University of São Paulo , São Paulo , Brazil.a Department of Nephrology, School of Medicine , University of São Paulo , São Paulo , Brazil.a Department of Nephrology, School of Medicine , University of São Paulo , São Paulo , Brazil.a Department of Nephrology, School of Medicine , University of São Paulo , São Paulo , Brazil.b Nephrology Division , Federal University of São Paulo , São Paulo , Brazil.b Nephrology Division , Federal University of São Paulo , São Paulo , Brazil.a Department of Nephrology, School of Medicine , University of São Paulo , São Paulo , Brazil.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27845599

Citation

Shimizu, Maria Heloisa Massola, et al. "N-acetylcysteine Protects Against Star Fruit-induced Acute Kidney Injury." Renal Failure, vol. 39, no. 1, 2017, pp. 193-202.
Shimizu MH, Gois PH, Volpini RA, et al. N-acetylcysteine protects against star fruit-induced acute kidney injury. Ren Fail. 2017;39(1):193-202.
Shimizu, M. H., Gois, P. H., Volpini, R. A., Canale, D., Luchi, W. M., Froeder, L., Heilberg, I. P., & Seguro, A. C. (2017). N-acetylcysteine protects against star fruit-induced acute kidney injury. Renal Failure, 39(1), 193-202. https://doi.org/10.1080/0886022X.2016.1256315
Shimizu MH, et al. N-acetylcysteine Protects Against Star Fruit-induced Acute Kidney Injury. Ren Fail. 2017;39(1):193-202. PubMed PMID: 27845599.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - N-acetylcysteine protects against star fruit-induced acute kidney injury. AU - Shimizu,Maria Heloisa Massola, AU - Gois,Pedro Henrique França, AU - Volpini,Rildo Aparecido, AU - Canale,Daniele, AU - Luchi,Weverton Machado, AU - Froeder,Leila, AU - Heilberg,Ita Pfeferman, AU - Seguro,Antonio Carlos, Y1 - 2016/11/15/ PY - 2016/11/16/pubmed PY - 2017/5/24/medline PY - 2016/11/16/entrez KW - Acute kidney injury KW - N-acetylcysteine KW - acute oxalate nephropathy KW - oxidative stress KW - star fruit SP - 193 EP - 202 JF - Renal failure JO - Ren Fail VL - 39 IS - 1 N2 - BACKGROUND: Star fruit (SF) is a popular fruit, commonly cultivated in many tropical countries, that contains large amount of oxalate. Acute oxalate nephropathy and direct renal tubular damage through release of free radicals are the main mechanisms involved in SF-induced acute kidney injury (AKI). The aim of this study was to evaluate the protective effect of N-acetylcysteine (NAC) on SF-induced nephrotoxicity due to its potent antioxidant effect. MATERIALS AND METHODS: Male Wistar rats received SF juice (4 mL/100 g body weight) by gavage after a 12 h fasting and water deprivation. Fasting and water deprivation continued for 6 h thereafter to warrant juice absorption. Thereafter, animals were allocated to three experimental groups: SF (n = 6): received tap water; SF + NAC (n = 6): received NAC (4.8 g/L) in drinking water for 48 h after gavage; and Sham (n = 6): no interventions. After 48 h, inulin clearance studies were performed to determine glomerular filtration rate. In a second series of experiment, rats were housed in metabolic cages for additional assessments. RESULTS: SF rats showed markedly reduced inulin clearance associated with hyperoxaluria, renal tubular damage, increased oxidative stress and inflammation. NAC treatment ameliorated all these alterations. Under polarized light microscopy, SF rats exhibited intense calcium oxalate birefringence crystals deposition, dilation of renal tubules and tubular epithelial degeneration, which were attenuate by NAC therapy. CONCLUSIONS: Our data show that therapeutic NAC attenuates renal dysfunction in a model of acute oxalate nephropathy following SF ingestion by reducing oxidative stress, oxaluria, and inflammation. This might represent a novel indication of NAC for the treatment of SF-induced AKI. SN - 1525-6049 UR - https://www.unboundmedicine.com/medline/citation/27845599/N_acetylcysteine_protects_against_star_fruit_induced_acute_kidney_injury_ L2 - https://www.tandfonline.com/doi/full/10.1080/0886022X.2016.1256315 DB - PRIME DP - Unbound Medicine ER -