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In-vitro alpha amylase inhibitory activity of the leaf extracts of Adenanthera pavonina.
BMC Complement Altern Med. 2016 Nov 15; 16(1):466.BC

Abstract

BACKGROUND

Diabetes has caused a major burden to the health sector in the developing countries and has shown an increasing trend among the urban population. It is estimated that most patients are with type II diabetes which could be easily treated with dietary changes, exercise, and medication. Sri Lanka carries a long history ayurvedic medicine where it uses the plant for treating many diseases. Therefore it is important to screen medicinal plants scientifically so they could be used safely and effectively in the traditional medical system and also be used for further investigations. Adenanthera pavonina is a plant used in the Ayurvedic medical system in Sri Lanka for treating many diseases including diabetics. We evaluated the anti-diabetic properties and the antioxidant properties of Adenanthera pavonina leaves.

METHODS

The methanol extract of the leaves was sequentially extracted with petroleum ether and thereafter was partitioned between EtOAc, and water. The α-amylase inhibition assay was performed using the 3,5- dinitrosalicylic acid method. The antioxidant activities were measured using the DPPH free radical scavenging activity and the total phenolic content using Folin-Ciocalteu's reagent. The cytotoxicity of the extract was evaluated using the Brine shrimp bioassay.

RESULTS

The IC50 values of α amylase inhibitory activity of MeOH, EtOAc, petroleum ether, and water were 16.16 ± 2.23, 59.93 ± 0.25, 145.49 ± 4.86 and 214.85 ± 9.72 μg/ml respectively and was similar to that of Acarbose (18.63 ± 1.21 (μg/ml). Antioxidant activities were also determined and the EtOAc fraction showed the highest total phenolic content (34. 62 ± 1.14 mg/g extract) and the highest DPPH scavenging activity with an IC50 of 249.92 ± 3.35 μg/ml.

CONCLUSION

The leaf extracts of Adenanthera pavonina exhibit remarkable α-amylase inhibitory activity in the crude methanolic extract. Hence leaves of Adenanthera pavonina has a potential to be used as a regular green vegetable and also be investigated further in isolating pure compounds with anti-diabetic activity.

Authors+Show Affiliations

Department of Physical Sciences and Technology, Faculty of Applied Sciences, Sabaragamuwa University of Sri Lanka, Belihuloya, Sri Lanka. meritanirmali@gmail.com.Department of Pharmacy Faculty of Allied Health Sciences, University of Peradeniya, Peradeniya, Sri Lanka.Faculty of Allied Health Sciences, University of Peradeniya, Peradeniya, Sri Lanka.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27846876

Citation

Wickramaratne, M Nirmali, et al. "In-vitro Alpha Amylase Inhibitory Activity of the Leaf Extracts of Adenanthera Pavonina." BMC Complementary and Alternative Medicine, vol. 16, no. 1, 2016, p. 466.
Wickramaratne MN, Punchihewa JC, Wickramaratne DB. In-vitro alpha amylase inhibitory activity of the leaf extracts of Adenanthera pavonina. BMC Complement Altern Med. 2016;16(1):466.
Wickramaratne, M. N., Punchihewa, J. C., & Wickramaratne, D. B. (2016). In-vitro alpha amylase inhibitory activity of the leaf extracts of Adenanthera pavonina. BMC Complementary and Alternative Medicine, 16(1), 466.
Wickramaratne MN, Punchihewa JC, Wickramaratne DB. In-vitro Alpha Amylase Inhibitory Activity of the Leaf Extracts of Adenanthera Pavonina. BMC Complement Altern Med. 2016 Nov 15;16(1):466. PubMed PMID: 27846876.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In-vitro alpha amylase inhibitory activity of the leaf extracts of Adenanthera pavonina. AU - Wickramaratne,M Nirmali, AU - Punchihewa,J C, AU - Wickramaratne,D B M, Y1 - 2016/11/15/ PY - 2016/07/25/received PY - 2016/11/09/accepted PY - 2016/11/17/entrez PY - 2016/11/17/pubmed PY - 2017/1/14/medline SP - 466 EP - 466 JF - BMC complementary and alternative medicine JO - BMC Complement Altern Med VL - 16 IS - 1 N2 - BACKGROUND: Diabetes has caused a major burden to the health sector in the developing countries and has shown an increasing trend among the urban population. It is estimated that most patients are with type II diabetes which could be easily treated with dietary changes, exercise, and medication. Sri Lanka carries a long history ayurvedic medicine where it uses the plant for treating many diseases. Therefore it is important to screen medicinal plants scientifically so they could be used safely and effectively in the traditional medical system and also be used for further investigations. Adenanthera pavonina is a plant used in the Ayurvedic medical system in Sri Lanka for treating many diseases including diabetics. We evaluated the anti-diabetic properties and the antioxidant properties of Adenanthera pavonina leaves. METHODS: The methanol extract of the leaves was sequentially extracted with petroleum ether and thereafter was partitioned between EtOAc, and water. The α-amylase inhibition assay was performed using the 3,5- dinitrosalicylic acid method. The antioxidant activities were measured using the DPPH free radical scavenging activity and the total phenolic content using Folin-Ciocalteu's reagent. The cytotoxicity of the extract was evaluated using the Brine shrimp bioassay. RESULTS: The IC50 values of α amylase inhibitory activity of MeOH, EtOAc, petroleum ether, and water were 16.16 ± 2.23, 59.93 ± 0.25, 145.49 ± 4.86 and 214.85 ± 9.72 μg/ml respectively and was similar to that of Acarbose (18.63 ± 1.21 (μg/ml). Antioxidant activities were also determined and the EtOAc fraction showed the highest total phenolic content (34. 62 ± 1.14 mg/g extract) and the highest DPPH scavenging activity with an IC50 of 249.92 ± 3.35 μg/ml. CONCLUSION: The leaf extracts of Adenanthera pavonina exhibit remarkable α-amylase inhibitory activity in the crude methanolic extract. Hence leaves of Adenanthera pavonina has a potential to be used as a regular green vegetable and also be investigated further in isolating pure compounds with anti-diabetic activity. SN - 1472-6882 UR - https://www.unboundmedicine.com/medline/citation/27846876/In_vitro_alpha_amylase_inhibitory_activity_of_the_leaf_extracts_of_Adenanthera_pavonina_ L2 - https://bmccomplementalternmed.biomedcentral.com/articles/10.1186/s12906-016-1452-y DB - PRIME DP - Unbound Medicine ER -