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The Lnc RNA SPRY4-IT1 Modulates Trophoblast Cell Invasion and Migration by Affecting the Epithelial-Mesenchymal Transition.
Sci Rep 2016; 6:37183SR

Abstract

Preeclampsia is a common, pregnancy-specific disease and a major contributor to maternal and foetal morbidity and mortality. Some placental abnormalities, including deficient implantation, abnormal trophoblast cell function, and improper placental vascular development, are believed to lead to preeclampsia. The long noncoding RNA SPRY4-IT1 is more highly expressed in preeclamptic human placentas than in normal placentas. We assessed the role of epithelial-mesenchymal transition (EMT)-associated invasion and migration in HTR-8/SVneo trophoblast cells. Overexpression of SPRY4-IT1 suppressed trophoblast cell migration and invasion, whereas reduced expression of SPRY4-IT1 prevented the EMT process. Mechanistically, an RNA immunoprecipitation experiment showed that SPRY4-IT1 bound directly to HuR and mediated the β-catenin expression associated with EMT in HTR-8/SVneo cells. Moreover, the expression levels of genes in the WNT family, such as WNT3 and WNT5B, were changed after transfection of HTR-8/SVneo with SPRY4-IT1. Together, our results highlight the roles of SPRY4-IT1 in causing trophoblast cell dysfunction by acting through the Wnt/β-catenin pathway, and consequently in impairing spiral artery remodelling. These results suggest a new potential therapeutic target for intervention against preeclampsia.

Authors+Show Affiliations

Department of Obstetrics and Gynecology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, JiangSu Province, 210029, China.Department of Obstetrics and Gynecology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, JiangSu Province, 210029, China.Departments of Obstetrics and Gynecology, Yuhuangding Hospital of Yantai, Yantai, Shandong Province, 264000, China.Department of Obstetrics and Gynecology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, JiangSu Province, 210029, China.Department of Obstetrics and Gynecology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, JiangSu Province, 210029, China.Department of Obstetrics and Gynecology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, JiangSu Province, 210029, China.The Family Planning Science and Technology Research Institute of Jiangsu province, Nanjing, JiangSu Province, 210029, China.Department of Obstetrics and Gynecology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, JiangSu Province, 210029, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

27853262

Citation

Zuo, Qing, et al. "The Lnc RNA SPRY4-IT1 Modulates Trophoblast Cell Invasion and Migration By Affecting the Epithelial-Mesenchymal Transition." Scientific Reports, vol. 6, 2016, p. 37183.
Zuo Q, Huang S, Zou Y, et al. The Lnc RNA SPRY4-IT1 Modulates Trophoblast Cell Invasion and Migration by Affecting the Epithelial-Mesenchymal Transition. Sci Rep. 2016;6:37183.
Zuo, Q., Huang, S., Zou, Y., Xu, Y., Jiang, Z., Zou, S., ... Sun, L. (2016). The Lnc RNA SPRY4-IT1 Modulates Trophoblast Cell Invasion and Migration by Affecting the Epithelial-Mesenchymal Transition. Scientific Reports, 6, p. 37183. doi:10.1038/srep37183.
Zuo Q, et al. The Lnc RNA SPRY4-IT1 Modulates Trophoblast Cell Invasion and Migration By Affecting the Epithelial-Mesenchymal Transition. Sci Rep. 2016 11 17;6:37183. PubMed PMID: 27853262.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The Lnc RNA SPRY4-IT1 Modulates Trophoblast Cell Invasion and Migration by Affecting the Epithelial-Mesenchymal Transition. AU - Zuo,Qing, AU - Huang,Shiyun, AU - Zou,Yanfen, AU - Xu,Yetao, AU - Jiang,Ziyan, AU - Zou,Shan, AU - Xu,Haoqing, AU - Sun,Lizhou, Y1 - 2016/11/17/ PY - 2016/03/21/received PY - 2016/10/26/accepted PY - 2016/11/18/entrez PY - 2016/11/18/pubmed PY - 2018/5/18/medline SP - 37183 EP - 37183 JF - Scientific reports JO - Sci Rep VL - 6 N2 - Preeclampsia is a common, pregnancy-specific disease and a major contributor to maternal and foetal morbidity and mortality. Some placental abnormalities, including deficient implantation, abnormal trophoblast cell function, and improper placental vascular development, are believed to lead to preeclampsia. The long noncoding RNA SPRY4-IT1 is more highly expressed in preeclamptic human placentas than in normal placentas. We assessed the role of epithelial-mesenchymal transition (EMT)-associated invasion and migration in HTR-8/SVneo trophoblast cells. Overexpression of SPRY4-IT1 suppressed trophoblast cell migration and invasion, whereas reduced expression of SPRY4-IT1 prevented the EMT process. Mechanistically, an RNA immunoprecipitation experiment showed that SPRY4-IT1 bound directly to HuR and mediated the β-catenin expression associated with EMT in HTR-8/SVneo cells. Moreover, the expression levels of genes in the WNT family, such as WNT3 and WNT5B, were changed after transfection of HTR-8/SVneo with SPRY4-IT1. Together, our results highlight the roles of SPRY4-IT1 in causing trophoblast cell dysfunction by acting through the Wnt/β-catenin pathway, and consequently in impairing spiral artery remodelling. These results suggest a new potential therapeutic target for intervention against preeclampsia. SN - 2045-2322 UR - https://www.unboundmedicine.com/medline/citation/27853262/The_Lnc_RNA_SPRY4_IT1_Modulates_Trophoblast_Cell_Invasion_and_Migration_by_Affecting_the_Epithelial_Mesenchymal_Transition_ L2 - http://dx.doi.org/10.1038/srep37183 DB - PRIME DP - Unbound Medicine ER -