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An antidiabetic polyherbal phytomedicine confers stress resistance and extends lifespan in Caenorhabditis elegans.
Biogerontology 2017; 18(1):131-147B

Abstract

An Ayurvedic polyherbal extract (PHE) comprising six herbs viz. Berberis aristata, Cyperus rotundus, Cedrus deodara, Emblica officinalis, Terminalia chebula and Terminalia bellirica is mentioned as an effective anti-hyperglycemic agent in 'Charaka Samhita', the classical text of Ayurveda. Previously, antidiabetic drug metformin was found to elicit antiaging effects and PHE was also found to exhibit antidiabetic effects in humans. Therefore, we screened it for its in vivo antioxidant antiaging effect on stress and lifespan using human homologous Caenorhabditis elegans model system. The effect on aging is evaluated by studying effect of PHE on mean survival in worms. The stress modulatory potential was assessed by quantification of intracellular ROS level, autofluorescent age pigment lipofuscin, oxidative and thermal stress assays. Additionally, stress response was quantified using gene reporter assays. The 0.01 µg/ml dose of PHE was able to enhance mean lifespan by 16.09% (P < 0.0001) in C. elegans. Furthermore, PHE treated worms demonstrated oxidative stress resistance in both wild type and stress hypersensitive mev-1 mutant along with upregulation of stress response genes sod-3 and gst-4. The delayed aging under stress can be attributed to its direct reactive oxygen species-scavenging activity and regulation of some age associated genes like daf-2, daf-16, skn-1, sod-3 and gst-4 in wild-type worms. Additonally, PHE delayed age related paralysis phenotype in CL4176 transgenic worms. Altogether, our results suggest PHE significantly improves the oxidative stress and life span in C. elegans. Overall the present study suggests this polyherbal formulation might play important role in regultaing aging and related complications like diabetes.

Authors+Show Affiliations

Microbial Technology and Nematology Department, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow, 226 015, India.Microbial Technology and Nematology Department, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow, 226 015, India.Department of Herbal Medicinal Products, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow, 226 015, India.Department of Herbal Medicinal Products, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow, 226 015, India.Microbial Technology and Nematology Department, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow, 226 015, India. r.pandey@cimap.res.in.

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

27853905

Citation

Rathor, Laxmi, et al. "An Antidiabetic Polyherbal Phytomedicine Confers Stress Resistance and Extends Lifespan in Caenorhabditis Elegans." Biogerontology, vol. 18, no. 1, 2017, pp. 131-147.
Rathor L, Pant A, Awasthi H, et al. An antidiabetic polyherbal phytomedicine confers stress resistance and extends lifespan in Caenorhabditis elegans. Biogerontology. 2017;18(1):131-147.
Rathor, L., Pant, A., Awasthi, H., Mani, D., & Pandey, R. (2017). An antidiabetic polyherbal phytomedicine confers stress resistance and extends lifespan in Caenorhabditis elegans. Biogerontology, 18(1), pp. 131-147. doi:10.1007/s10522-016-9668-2.
Rathor L, et al. An Antidiabetic Polyherbal Phytomedicine Confers Stress Resistance and Extends Lifespan in Caenorhabditis Elegans. Biogerontology. 2017;18(1):131-147. PubMed PMID: 27853905.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - An antidiabetic polyherbal phytomedicine confers stress resistance and extends lifespan in Caenorhabditis elegans. AU - Rathor,Laxmi, AU - Pant,Aakanksha, AU - Awasthi,Harshika, AU - Mani,Dayanandan, AU - Pandey,Rakesh, Y1 - 2016/11/16/ PY - 2016/06/24/received PY - 2016/11/06/accepted PY - 2016/11/18/pubmed PY - 2017/10/19/medline PY - 2016/11/18/entrez KW - Aging KW - Anti-diabetic KW - Caenorhabditis elegans KW - Neurodegenerative disease KW - Polyherbal extract SP - 131 EP - 147 JF - Biogerontology JO - Biogerontology VL - 18 IS - 1 N2 - An Ayurvedic polyherbal extract (PHE) comprising six herbs viz. Berberis aristata, Cyperus rotundus, Cedrus deodara, Emblica officinalis, Terminalia chebula and Terminalia bellirica is mentioned as an effective anti-hyperglycemic agent in 'Charaka Samhita', the classical text of Ayurveda. Previously, antidiabetic drug metformin was found to elicit antiaging effects and PHE was also found to exhibit antidiabetic effects in humans. Therefore, we screened it for its in vivo antioxidant antiaging effect on stress and lifespan using human homologous Caenorhabditis elegans model system. The effect on aging is evaluated by studying effect of PHE on mean survival in worms. The stress modulatory potential was assessed by quantification of intracellular ROS level, autofluorescent age pigment lipofuscin, oxidative and thermal stress assays. Additionally, stress response was quantified using gene reporter assays. The 0.01 µg/ml dose of PHE was able to enhance mean lifespan by 16.09% (P < 0.0001) in C. elegans. Furthermore, PHE treated worms demonstrated oxidative stress resistance in both wild type and stress hypersensitive mev-1 mutant along with upregulation of stress response genes sod-3 and gst-4. The delayed aging under stress can be attributed to its direct reactive oxygen species-scavenging activity and regulation of some age associated genes like daf-2, daf-16, skn-1, sod-3 and gst-4 in wild-type worms. Additonally, PHE delayed age related paralysis phenotype in CL4176 transgenic worms. Altogether, our results suggest PHE significantly improves the oxidative stress and life span in C. elegans. Overall the present study suggests this polyherbal formulation might play important role in regultaing aging and related complications like diabetes. SN - 1573-6768 UR - https://www.unboundmedicine.com/medline/citation/27853905/An_antidiabetic_polyherbal_phytomedicine_confers_stress_resistance_and_extends_lifespan_in_Caenorhabditis_elegans_ L2 - https://doi.org/10.1007/s10522-016-9668-2 DB - PRIME DP - Unbound Medicine ER -