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The biochemical characterization, stabilization studies and the antiproliferative effect of bromelain against B16F10 murine melanoma cells.
Int J Food Sci Nutr 2017; 68(4):442-454IJ

Abstract

The current study aims to extract bromelain from different parts (stem, crown, peels, pulp and leaves) of Ananas comosus var. comosus AGB 772; to determine of optimum pH and temperature; to test bromelain stability in disodium EDTA and sodium benzoate, and to investigate its pharmacological activity on B16F10 murine melanoma cells in vitro. The highest enzymatic activity was found in bromelain extracted from the pulp and peel. The optimum bromelain pH among all studied pineapple parts was 6.0. The optimum temperature was above 50 °C in all bromelain extracts. The fluorescence analysis confirmed the stability of bromelain in the presence of EDTA and sodium benzoate. Bromelain was pharmacologically active against B16F10 melanoma cells and it was possible verifying approximately 100% inhibition of tumor cell proliferation in vitro. Since bromelain activity was found in different parts of pineapple plants, pineapple residues from the food industry may be used for bromelain extraction.

Authors+Show Affiliations

a Health Department , State University of Feira de Santana (UEFS) , Feira de Santana , Brazil.b Parasitic Biology Laboratory , Oswaldo Cruz Foundation (FIOCRUZ) , Salvador , Brazil.c Embrapa Mandioca e Fruticultura , Cruz das Almas , Brazil.b Parasitic Biology Laboratory , Oswaldo Cruz Foundation (FIOCRUZ) , Salvador , Brazil.d Biological Sciences Department , Biotechnology and Genetics Center, State University of Santa Cruz , Ilhéus , Brazil.e Laboratory of Optical Properties, Physics Department , Federal University of Bahia , Salvador , Brazil.e Laboratory of Optical Properties, Physics Department , Federal University of Bahia , Salvador , Brazil.d Biological Sciences Department , Biotechnology and Genetics Center, State University of Santa Cruz , Ilhéus , Brazil.f Technology, Sanitation, Hydric Resources and Environment Department , State University of Feira de Santana (UEFS) , Feira de Santana , Brazil.a Health Department , State University of Feira de Santana (UEFS) , Feira de Santana , Brazil.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

27855525

Citation

São Paulo Barretto Miranda, Íngara Keisle, et al. "The Biochemical Characterization, Stabilization Studies and the Antiproliferative Effect of Bromelain Against B16F10 Murine Melanoma Cells." International Journal of Food Sciences and Nutrition, vol. 68, no. 4, 2017, pp. 442-454.
São Paulo Barretto Miranda ÍK, Fontes Suzart Miranda A, Souza FV, et al. The biochemical characterization, stabilization studies and the antiproliferative effect of bromelain against B16F10 murine melanoma cells. Int J Food Sci Nutr. 2017;68(4):442-454.
São Paulo Barretto Miranda, Í. K., Fontes Suzart Miranda, A., Souza, F. V., Vannier-Santos, M. A., Pirovani, C. P., Pepe, I. M., ... de Assis, S. A. (2017). The biochemical characterization, stabilization studies and the antiproliferative effect of bromelain against B16F10 murine melanoma cells. International Journal of Food Sciences and Nutrition, 68(4), pp. 442-454. doi:10.1080/09637486.2016.1254599.
São Paulo Barretto Miranda ÍK, et al. The Biochemical Characterization, Stabilization Studies and the Antiproliferative Effect of Bromelain Against B16F10 Murine Melanoma Cells. Int J Food Sci Nutr. 2017;68(4):442-454. PubMed PMID: 27855525.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The biochemical characterization, stabilization studies and the antiproliferative effect of bromelain against B16F10 murine melanoma cells. AU - São Paulo Barretto Miranda,Íngara Keisle, AU - Fontes Suzart Miranda,Anderson, AU - Souza,Fernanda Vidigal Duarte, AU - Vannier-Santos,Marcos André, AU - Pirovani,Carlos Priminho, AU - Pepe,Iuri Muniz, AU - Rodowanski,Ivanoé João, AU - Ferreira,Katiúcia Tícila de Souza Eduvirgens, AU - Mendes Souza Vaz,Luciano, AU - de Assis,Sandra Aparecida, Y1 - 2016/11/17/ PY - 2016/11/20/pubmed PY - 2017/9/7/medline PY - 2016/11/19/entrez KW - Antiproliferative effect in vitro KW - bromelain KW - cell culture KW - enzyme stability KW - murine melanoma cells KW - response surface methodology SP - 442 EP - 454 JF - International journal of food sciences and nutrition JO - Int J Food Sci Nutr VL - 68 IS - 4 N2 - The current study aims to extract bromelain from different parts (stem, crown, peels, pulp and leaves) of Ananas comosus var. comosus AGB 772; to determine of optimum pH and temperature; to test bromelain stability in disodium EDTA and sodium benzoate, and to investigate its pharmacological activity on B16F10 murine melanoma cells in vitro. The highest enzymatic activity was found in bromelain extracted from the pulp and peel. The optimum bromelain pH among all studied pineapple parts was 6.0. The optimum temperature was above 50 °C in all bromelain extracts. The fluorescence analysis confirmed the stability of bromelain in the presence of EDTA and sodium benzoate. Bromelain was pharmacologically active against B16F10 melanoma cells and it was possible verifying approximately 100% inhibition of tumor cell proliferation in vitro. Since bromelain activity was found in different parts of pineapple plants, pineapple residues from the food industry may be used for bromelain extraction. SN - 1465-3478 UR - https://www.unboundmedicine.com/medline/citation/27855525/The_biochemical_characterization_stabilization_studies_and_the_antiproliferative_effect_of_bromelain_against_B16F10_murine_melanoma_cells_ L2 - http://www.tandfonline.com/doi/full/10.1080/09637486.2016.1254599 DB - PRIME DP - Unbound Medicine ER -